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The map label for this gene is lacF [H]

Identifier: 226223493

GI number: 226223493

Start: 914759

End: 915088

Strand: Direct

Name: lacF [H]

Synonym: Lm4b_00892

Alternate gene names: 226223493

Gene position: 914759-915088 (Clockwise)

Preceding gene: 226223492

Following gene: 226223494

Centisome position: 31.41

GC content: 33.94

Gene sequence:

>330_bases
ATGGACATAGAAAAAATATCGTTTTCATTAATTTCACTGGCGGGGGATTCGTTTTCTAAATTAATTGAAGCCTTACATGC
CGCAAAAGAATCAGATTCAGAATTAGTAGAACAACTTTTAAAAGAAGCAGATGACCTAATGATTGAAGCGCACAAAGTAC
AAACGGAAATGCTGATTCAAGAAACAAGAGGGGAACAAGCAAATTTTTCCGTTTTACTCGTACATGCGCAAGATACTTTA
ATGAATACCATCTTAGCATCGACTTTAATTCGAGAAATGATTGAAATGCATCAAGAGATTAAAACATTAAGAAAAGAGGA
GGAAAAGTAA

Upstream 100 bases:

>100_bases
TGGACGAGGAAAAAATGCAAAAAATTAAAATGGCTAGCTCAAAACGTGATATTTATCATTACGTTAAGTCGGCTATCTTT
GAATGAGAGAGGGTTTCATA

Downstream 100 bases:

>100_bases
ATGGAAATCAATCAATTACATGTACTTTTAGTGTGTAACTTAGGCGCATCTACAGGGGTAATGGTTACAAAAATGAAGGA
AATCGCCCAAAACAGTGAAA

Product: PTS system enzyme IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Lac; EIII-Lac; PTS system lactose-specific EIIA component [H]

Number of amino acids: Translated: 109; Mature: 109

Protein sequence:

>109_residues
MDIEKISFSLISLAGDSFSKLIEALHAAKESDSELVEQLLKEADDLMIEAHKVQTEMLIQETRGEQANFSVLLVHAQDTL
MNTILASTLIREMIEMHQEIKTLRKEEEK

Sequences:

>Translated_109_residues
MDIEKISFSLISLAGDSFSKLIEALHAAKESDSELVEQLLKEADDLMIEAHKVQTEMLIQETRGEQANFSVLLVHAQDTL
MNTILASTLIREMIEMHQEIKTLRKEEEK
>Mature_109_residues
MDIEKISFSLISLAGDSFSKLIEALHAAKESDSELVEQLLKEADDLMIEAHKVQTEMLIQETRGEQANFSVLLVHAQDTL
MNTILASTLIREMIEMHQEIKTLRKEEEK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 12412; Mature: 12412

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEKISFSLISLAGDSFSKLIEALHAAKESDSELVEQLLKEADDLMIEAHKVQTEMLIQ
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETRGEQANFSVLLVHAQDTLMNTILASTLIREMIEMHQEIKTLRKEEEK
HHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIEKISFSLISLAGDSFSKLIEALHAAKESDSELVEQLLKEADDLMIEAHKVQTEMLIQ
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETRGEQANFSVLLVHAQDTLMNTILASTLIREMIEMHQEIKTLRKEEEK
HHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA