Click here to switch to the map view.

The map label for this gene is ddl

Identifier: 226223473

GI number: 226223473

Start: 893027

End: 894139

Strand: Direct

Name: ddl

Synonym: Lm4b_00871

Alternate gene names: 226223473

Gene position: 893027-894139 (Clockwise)

Preceding gene: 226223472

Following gene: 226223474

Centisome position: 30.66

GC content: 40.97

Gene sequence:

>1113_bases
ATGAAAACCAAATTAATTTTGTTATACGGTGGGAAATCTGCTGAACACGAAGTTTCCTTACAAACAGCATTTTCAGTTAT
CAATGCTTTGGATTTAGAAAAATTCGAAGCTGCGCCAATATATATTACGAATGAAGGTGAGTGGATTCAAGGGCCACTTC
TTTCTGGGAAGTTAGATTTTGTCGAACAATTACGTTTTTCAGCTACAGATACAATAAAACTTGCAACAACTGAATCAGAA
AAATCAGAAGGGGAAGCGATTAGCCCGGCCGTTTTAGAAGCAGACGGACAAGAGACAGTCGTATTCCCGCTTTTACATGG
TCCAAACGGAGAAGATGGCACTGTTCAAGGATTGTTCGAAGTATTAAACATTCCATATGTTGGTAACGGGGTCTTGGCAT
CTTCTGCTGCAATGGACAAAATCGTGATGAAGAAAATCTTTGCAGATGCTGGAATCCCGCAAGTTCCAGCAGTTGCTGTT
CGATTAATTGATTGGAAGAACTATCAAGCGGAAATGGTTGCGGAGATGGAAGAAGTACTTACCTATCCAGTTTTCGTAAA
ACCAGCAAACCTTGGTTCGAGTGTTGGTATTAGTAAGGCAACGAACAAAAAAGAATTAGCGGACGCAATGACAGAAGCAT
TTTTATATGACCGTCGTGTGGTTGTTGAACAAGGTGTTGTTGCGCGCGAAATCGAAATGGGCGTACTTGGAAATGATACG
CCAGTTTGCTCTGTTCCGGGTGAAATTTTGCCAGAAGGTGCAGTTGCTACGTTTTACGATTACAAAGCAAAATACCAGGA
TAATAATACTGCGTTAATTATTCCGACTGAAGTAGATCCGGAAATCTTGGAACAAATGAAAGAATATGCGGTTCAAGCAT
TCCTAGGGCTTGATGCAAGCGGACTAGTGCGAGCGGACTTTTTCTTAACGGAAGATAATCAGTTATTCTTAAATGAAGTC
AATACAATGCCTGGTTTCACACCGTACAGCATGTATCCTCTTCTTTGGCAGGAAACGGGCTTACCGTACGGAGCGTTAAT
TGAACGATTAGTCGATTTAGCGAAAGAACGCCACGCAGCCAAAAATGCACTTAAATATAAATTAGAAGACTAA

Upstream 100 bases:

>100_bases
TTTAAAAAAGAAGATTTTTTCAACCTATTTTTTAGTTATACCTTTTCATCCTGTCAAAACATGGTAAAATAGATAAAGCG
AATAATGACGGAGGGGTATT

Downstream 100 bases:

>100_bases
AATAAACAAGAAAGCAGTTTTCATATGCGGAAGCTGCTTTTTTATGGAAGAGAGCGATTTAATTTGAAAAAAACAGTAGG
CGAAGTAGTAGCAATGTTAG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKTKLILLYGGKSAEHEVSLQTAFSVINALDLEKFEAAPIYITNEGEWIQGPLLSGKLDFVEQLRFSATDTIKLATTESE
KSEGEAISPAVLEADGQETVVFPLLHGPNGEDGTVQGLFEVLNIPYVGNGVLASSAAMDKIVMKKIFADAGIPQVPAVAV
RLIDWKNYQAEMVAEMEEVLTYPVFVKPANLGSSVGISKATNKKELADAMTEAFLYDRRVVVEQGVVAREIEMGVLGNDT
PVCSVPGEILPEGAVATFYDYKAKYQDNNTALIIPTEVDPEILEQMKEYAVQAFLGLDASGLVRADFFLTEDNQLFLNEV
NTMPGFTPYSMYPLLWQETGLPYGALIERLVDLAKERHAAKNALKYKLED

Sequences:

>Translated_370_residues
MKTKLILLYGGKSAEHEVSLQTAFSVINALDLEKFEAAPIYITNEGEWIQGPLLSGKLDFVEQLRFSATDTIKLATTESE
KSEGEAISPAVLEADGQETVVFPLLHGPNGEDGTVQGLFEVLNIPYVGNGVLASSAAMDKIVMKKIFADAGIPQVPAVAV
RLIDWKNYQAEMVAEMEEVLTYPVFVKPANLGSSVGISKATNKKELADAMTEAFLYDRRVVVEQGVVAREIEMGVLGNDT
PVCSVPGEILPEGAVATFYDYKAKYQDNNTALIIPTEVDPEILEQMKEYAVQAFLGLDASGLVRADFFLTEDNQLFLNEV
NTMPGFTPYSMYPLLWQETGLPYGALIERLVDLAKERHAAKNALKYKLED
>Mature_370_residues
MKTKLILLYGGKSAEHEVSLQTAFSVINALDLEKFEAAPIYITNEGEWIQGPLLSGKLDFVEQLRFSATDTIKLATTESE
KSEGEAISPAVLEADGQETVVFPLLHGPNGEDGTVQGLFEVLNIPYVGNGVLASSAAMDKIVMKKIFADAGIPQVPAVAV
RLIDWKNYQAEMVAEMEEVLTYPVFVKPANLGSSVGISKATNKKELADAMTEAFLYDRRVVVEQGVVAREIEMGVLGNDT
PVCSVPGEILPEGAVATFYDYKAKYQDNNTALIIPTEVDPEILEQMKEYAVQAFLGLDASGLVRADFFLTEDNQLFLNEV
NTMPGFTPYSMYPLLWQETGLPYGALIERLVDLAKERHAAKNALKYKLED

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=372, Percent_Identity=41.9354838709677, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1786280, Length=358, Percent_Identity=31.8435754189944, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 40570; Mature: 40570

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKLILLYGGKSAEHEVSLQTAFSVINALDLEKFEAAPIYITNEGEWIQGPLLSGKLDF
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCCHHHH
VEQLRFSATDTIKLATTESEKSEGEAISPAVLEADGQETVVFPLLHGPNGEDGTVQGLFE
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCEEEEEEEECCCCCCCHHHHHHH
VLNIPYVGNGVLASSAAMDKIVMKKIFADAGIPQVPAVAVRLIDWKNYQAEMVAEMEEVL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHHHHHHH
TYPVFVKPANLGSSVGISKATNKKELADAMTEAFLYDRRVVVEQGVVAREIEMGVLGNDT
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCCC
PVCSVPGEILPEGAVATFYDYKAKYQDNNTALIIPTEVDPEILEQMKEYAVQAFLGLDAS
CEEECCHHHCCCCCEEEEEECEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
GLVRADFFLTEDNQLFLNEVNTMPGFTPYSMYPLLWQETGLPYGALIERLVDLAKERHAA
CCEEEEEEEECCCEEEEEHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KNALKYKLED
HHHHEEECCC
>Mature Secondary Structure
MKTKLILLYGGKSAEHEVSLQTAFSVINALDLEKFEAAPIYITNEGEWIQGPLLSGKLDF
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEECCCCCCHHHH
VEQLRFSATDTIKLATTESEKSEGEAISPAVLEADGQETVVFPLLHGPNGEDGTVQGLFE
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCEEEEEEEECCCCCCCHHHHHHH
VLNIPYVGNGVLASSAAMDKIVMKKIFADAGIPQVPAVAVRLIDWKNYQAEMVAEMEEVL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHHHHHHH
TYPVFVKPANLGSSVGISKATNKKELADAMTEAFLYDRRVVVEQGVVAREIEMGVLGNDT
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCCC
PVCSVPGEILPEGAVATFYDYKAKYQDNNTALIIPTEVDPEILEQMKEYAVQAFLGLDAS
CEEECCHHHCCCCCEEEEEECEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
GLVRADFFLTEDNQLFLNEVNTMPGFTPYSMYPLLWQETGLPYGALIERLVDLAKERHAA
CCEEEEEEEECCCEEEEEHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KNALKYKLED
HHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]