Click here to switch to the map view.

The map label for this gene is spoT [H]

Identifier: 226223429

GI number: 226223429

Start: 839222

End: 839764

Strand: Direct

Name: spoT [H]

Synonym: Lm4b_00827

Alternate gene names: 226223429

Gene position: 839222-839764 (Clockwise)

Preceding gene: 226223428

Following gene: 226223430

Centisome position: 28.81

GC content: 42.73

Gene sequence:

>543_bases
ATGTATGAAAAAGCGAGACAGATGATGATCAAAGCGCATAGTGGCCAAGTTCGCAAAATTACTGGCGAGCCTTATTTTTC
GCATCCGTTGAACGTAGCGAGAATTTTGCGCCGCGCTGGTTTTCGCGAAGAAGTTGTTGTTGCGGGATTACTGCACGATG
CCGTTGAGGATACGGAAATGACCGATGCCGATATTCGCGCGGCTTTTGGCGCTGAAGTAGCCGATTTAGTAGCGTCTCAT
ACAGAAAATAAAACCTTATCTTGGGAAGAGCGAAAAGCACACACAATCGAACAAGTGCGCACCGGAAATTTAGAAGAAAA
AGCGTTAATCGTGGCGGATAAGCTAGACAATTTAACATCCGTCAAATATGCCTTAAGCTCAGAAGGAAAGTCTGTCTGGA
GCTATTTTAAACGCGGCTATGATCTGCAAAAATGGTACAACCAAGGGATTAAAAATAATATGGAATACGGATTAAATCCA
TCTGAAATCCCTCCATTTTTTGGTGAATATGCGCGACTTGTGAAGTGGATTTTTAAGAAGTAA

Upstream 100 bases:

>100_bases
TTAAATGGTCGTGAAATTACTTTTTATAATTAAAAAACTAGAAAGCCTCGCTCGAATCTTGTATAGTATAGACATAGATT
CTGAGTGGAGGCTTCTTTTT

Downstream 100 bases:

>100_bases
ATTTTGTATTTAAATATCTGTTAAATTAGGTATTTAGAATTAAAATTATAAGTTTTTAGAAAATAAGTTTCTTTTTCCAG
AAATCTATTGTAAAATGAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MYEKARQMMIKAHSGQVRKITGEPYFSHPLNVARILRRAGFREEVVVAGLLHDAVEDTEMTDADIRAAFGAEVADLVASH
TENKTLSWEERKAHTIEQVRTGNLEEKALIVADKLDNLTSVKYALSSEGKSVWSYFKRGYDLQKWYNQGIKNNMEYGLNP
SEIPPFFGEYARLVKWIFKK

Sequences:

>Translated_180_residues
MYEKARQMMIKAHSGQVRKITGEPYFSHPLNVARILRRAGFREEVVVAGLLHDAVEDTEMTDADIRAAFGAEVADLVASH
TENKTLSWEERKAHTIEQVRTGNLEEKALIVADKLDNLTSVKYALSSEGKSVWSYFKRGYDLQKWYNQGIKNNMEYGLNP
SEIPPFFGEYARLVKWIFKK
>Mature_180_residues
MYEKARQMMIKAHSGQVRKITGEPYFSHPLNVARILRRAGFREEVVVAGLLHDAVEDTEMTDADIRAAFGAEVADLVASH
TENKTLSWEERKAHTIEQVRTGNLEEKALIVADKLDNLTSVKYALSSEGKSVWSYFKRGYDLQKWYNQGIKNNMEYGLNP
SEIPPFFGEYARLVKWIFKK

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=111, Percent_Identity=45.045045045045, Blast_Score=74, Evalue=9e-14,
Organism=Escherichia coli, GI1790082, Length=89, Percent_Identity=40.4494382022472, Blast_Score=66, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25143118, Length=160, Percent_Identity=33.125, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650996, Length=113, Percent_Identity=40.7079646017699, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811 [H]

Pfam domain/function: PF04607 RelA_SpoT [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 20643; Mature: 20643

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEKARQMMIKAHSGQVRKITGEPYFSHPLNVARILRRAGFREEVVVAGLLHDAVEDTEM
CCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
TDADIRAAFGAEVADLVASHTENKTLSWEERKAHTIEQVRTGNLEEKALIVADKLDNLTS
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHH
VKYALSSEGKSVWSYFKRGYDLQKWYNQGIKNNMEYGLNPSEIPPFFGEYARLVKWIFKK
HHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYEKARQMMIKAHSGQVRKITGEPYFSHPLNVARILRRAGFREEVVVAGLLHDAVEDTEM
CCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
TDADIRAAFGAEVADLVASHTENKTLSWEERKAHTIEQVRTGNLEEKALIVADKLDNLTS
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHH
VKYALSSEGKSVWSYFKRGYDLQKWYNQGIKNNMEYGLNPSEIPPFFGEYARLVKWIFKK
HHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]