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The map label for this gene is gmuE [H]

Identifier: 226223430

GI number: 226223430

Start: 839900

End: 840772

Strand: Direct

Name: gmuE [H]

Synonym: Lm4b_00828

Alternate gene names: 226223430

Gene position: 839900-840772 (Clockwise)

Preceding gene: 226223429

Following gene: 226223431

Centisome position: 28.84

GC content: 42.61

Gene sequence:

>873_bases
ATGGTTTATGGAGCAATTGAAGCAGGCGGAACTAAGTTTGTTGTAGCAATCGGAGAAAAGTCAGGGAAAATTATTAAGCG
CGAGAGCTACCCGACAACGGAGCCAGCAGAAACAATGAAAGCAGTCATTCAATTTTTTAAACAGTATGAGGATGAATTAA
AAGCAATTGGGATTGGCTCATTTGGACCGATTGATATTCGGAAATCAAGTGCTACATACGGTTATATCACGCAAACACCA
AAACTTGCATGGCGCAATTATGATATCGTTGGTGCAATGAAAAAAGAATTTAATGTACCTATTGGTTTTACGACGGATGT
TAATGCAGCCGCTTTAGGAGAGGTAAGTTTAGGCGCGGCAGCAGGTTTGGATAGTTGTATTTATTTAACGATCGGAACAG
GAATCGGTGGTGGAGCAGTTGTTTCAGGCAAAATTCTTGAAGGCTTCTCCCACCCAGAAATGGGACATATTATGGTGCGC
CGACATAAGCGCGATCGTTTTACAGGCAGCTGCCCGAGTCATAGCGACTGTTTAGAGGGGCTTGCAGCAGGTGGAGCAAT
TGAAAAACGTTGGGGTCAAAAAGCAGCAGAACTTGCGGATAATGAAGAAGTGTGGAATTTAGAAGCGCATTATATCGCTC
AAGCTTTAATGAATTATACACTTATCTTATCCCCAGAAAGAATCGTGTTAGGTGGAGGCGTCATGAAACAACGCCAACTT
TTCCCACTTGTTCGTCAAAAATTGAAGGCACTCGTGAATAATTACGTGCAATTGCCGGATTTAGATGAATATATCGTTCC
TCCAAAGTTAGAAGATGACGCTGGAATTACAGGCTGTGTCTTGCTCGCTGTGGATGCTGAAGAGTCCAATTAA

Upstream 100 bases:

>100_bases
AGAATTAAAATTATAAGTTTTTAGAAAATAAGTTTCTTTTTCCAGAAATCTATTGTAAAATGAAAGCGTTATAAAACTTA
CGAGAAGGGAAGTTTTTTGA

Downstream 100 bases:

>100_bases
TGGCGAAAAACAGCTCTAGTTTATAGGGCTGTTTTTTGATATACTAAATTGTGATAAAACAAACAAGAACGCAGGAGGAA
AAAGATGTCCATTACGAACT

Product: fructokinase

Products: NA

Alternate protein names: Glucomannan utilization protein E [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MVYGAIEAGGTKFVVAIGEKSGKIIKRESYPTTEPAETMKAVIQFFKQYEDELKAIGIGSFGPIDIRKSSATYGYITQTP
KLAWRNYDIVGAMKKEFNVPIGFTTDVNAAALGEVSLGAAAGLDSCIYLTIGTGIGGGAVVSGKILEGFSHPEMGHIMVR
RHKRDRFTGSCPSHSDCLEGLAAGGAIEKRWGQKAAELADNEEVWNLEAHYIAQALMNYTLILSPERIVLGGGVMKQRQL
FPLVRQKLKALVNNYVQLPDLDEYIVPPKLEDDAGITGCVLLAVDAEESN

Sequences:

>Translated_290_residues
MVYGAIEAGGTKFVVAIGEKSGKIIKRESYPTTEPAETMKAVIQFFKQYEDELKAIGIGSFGPIDIRKSSATYGYITQTP
KLAWRNYDIVGAMKKEFNVPIGFTTDVNAAALGEVSLGAAAGLDSCIYLTIGTGIGGGAVVSGKILEGFSHPEMGHIMVR
RHKRDRFTGSCPSHSDCLEGLAAGGAIEKRWGQKAAELADNEEVWNLEAHYIAQALMNYTLILSPERIVLGGGVMKQRQL
FPLVRQKLKALVNNYVQLPDLDEYIVPPKLEDDAGITGCVLLAVDAEESN
>Mature_290_residues
MVYGAIEAGGTKFVVAIGEKSGKIIKRESYPTTEPAETMKAVIQFFKQYEDELKAIGIGSFGPIDIRKSSATYGYITQTP
KLAWRNYDIVGAMKKEFNVPIGFTTDVNAAALGEVSLGAAAGLDSCIYLTIGTGIGGGAVVSGKILEGFSHPEMGHIMVR
RHKRDRFTGSCPSHSDCLEGLAAGGAIEKRWGQKAAELADNEEVWNLEAHYIAQALMNYTLILSPERIVLGGGVMKQRQL
FPLVRQKLKALVNNYVQLPDLDEYIVPPKLEDDAGITGCVLLAVDAEESN

Specific function: Seems to be involved in the degradation of glucomannan [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI1786891, Length=287, Percent_Identity=23.6933797909408, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31371; Mature: 31371

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYGAIEAGGTKFVVAIGEKSGKIIKRESYPTTEPAETMKAVIQFFKQYEDELKAIGIGS
CEEEEEECCCEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCC
FGPIDIRKSSATYGYITQTPKLAWRNYDIVGAMKKEFNVPIGFTTDVNAAALGEVSLGAA
CCCCEEECCCCCEEEEECCCCCEECCCEEEEHHHHHCCCCCCCCCCCCCHHCCCCCCCHH
AGLDSCIYLTIGTGIGGGAVVSGKILEGFSHPEMGHIMVRRHKRDRFTGSCPSHSDCLEG
CCCCCEEEEEEECCCCCCEEEECHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHH
LAAGGAIEKRWGQKAAELADNEEVWNLEAHYIAQALMNYTLILSPERIVLGGGVMKQRQL
HHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCEEEEECCCEEEECCCCHHHHHH
FPLVRQKLKALVNNYVQLPDLDEYIVPPKLEDDAGITGCVLLAVDAEESN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCC
>Mature Secondary Structure
MVYGAIEAGGTKFVVAIGEKSGKIIKRESYPTTEPAETMKAVIQFFKQYEDELKAIGIGS
CEEEEEECCCEEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCC
FGPIDIRKSSATYGYITQTPKLAWRNYDIVGAMKKEFNVPIGFTTDVNAAALGEVSLGAA
CCCCEEECCCCCEEEEECCCCCEECCCEEEEHHHHHCCCCCCCCCCCCCHHCCCCCCCHH
AGLDSCIYLTIGTGIGGGAVVSGKILEGFSHPEMGHIMVRRHKRDRFTGSCPSHSDCLEG
CCCCCEEEEEEECCCCCCEEEECHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHH
LAAGGAIEKRWGQKAAELADNEEVWNLEAHYIAQALMNYTLILSPERIVLGGGVMKQRQL
HHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCEEEEECCCEEEECCCCHHHHHH
FPLVRQKLKALVNNYVQLPDLDEYIVPPKLEDDAGITGCVLLAVDAEESN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]