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The map label for this gene is ydaP [H]

Identifier: 226223353

GI number: 226223353

Start: 760076

End: 761806

Strand: Direct

Name: ydaP [H]

Synonym: Lm4b_00750

Alternate gene names: 226223353

Gene position: 760076-761806 (Clockwise)

Preceding gene: 226223351

Following gene: 226223354

Centisome position: 26.1

GC content: 42.75

Gene sequence:

>1731_bases
ATGAAAAAAGTAAAAGCAAGTGAAACACTCGTACAAACATTAAAAAATTGGGGGATCGACCACGTATATGGTTTACCTGG
TGATTCGATTGATACAGTAGTTGATGCCTTACGAAAAGAACAAGAAGCAATCGAGTTTATTCACGTTCGACACGAAGAAG
TGGCGACACTAGCCGCAGCAGCATATACCAAATTAACAGGCAAAATTGGTGTCGCATTATCTATCGGCGGCCCCGGAGCA
ATTCATCTTCTAAACGGGATGTATGATGCAAAAATGGATCATGTGCCAATGCTCGTACTCGCTGGGCAAGTGACAACCGA
CGTCTTAAACACCGGCTTTTTCCAAGAAGTCAATTTACCAGCGATTTTCGAAGATGTTGCCGTGTATAATAAACAAATTG
ATAATGCTGAAACACTCGCGGATGTGGTTGATGAAGCAATCCGCACCGCTTATAAAGAAAAAGGCGTCGCTGTCCTAACG
ATTCCAAATGATATTCCGTCACAAGTAATTAAAGCGAGCCTTGAAGCAAAACCAGTCAAATTCGAACAAGAAAATCCAAA
ACTAGATGAAGCGGCAATACAGGAAGCCGTAGCACGGATTGAAAAAGCGGAAAAACCAGTTATCTTAGCCGGATTAGGGA
CTAAACACGCTGGACCAGAACTAATCGCCTTCTCAGAAAAATTGAAAATCCCGATTATTCATTCCTTACCAGCAAAAACC
ATTGTTCCAGATAACCATCCAAATGCACTCGGAAACCTTGGTAAAATCGGAACCAAACCAGCATATGAAGCCATGCAAGA
AACTGACTTACTATTAATGTTCGGGAATGATTACCCTTATAGCGATTATTTACCAAAAAAAGCCGACTGCATCCAAATCG
ATATTGACCCAGCCAAAATCAGCAAACGTTTCCCCGCAACAGTTGGTTTAGTAGGCGATGCCGCAGAAATCATCGGTAAT
TTAACCGCGAAAACCGCACCTGTAGAAGAGCGTAAATTCCTTCAAGCATGCCAAGAAAACATGCAAGAATGGTGGAAATG
GTTAGAAGAAGACATCGCACAAACAACCGATCCAATCGCCCCAGAAGTCGTGATGGCGAACATCCAAAAAATCGCTGATA
AAGATGCCATTTTCTCTATCGACGTAGGAACTGCAACTGTTTGGAGCACTCGTTATTTACATTTAGCCCCACAAAATGAC
TTTATCGTTTCCGCGTGGCTTGGTACTATGGGCTGCGGTTTACCTGGAGCTATCGCTGCCAAAAAAGCATTCCCAGACCG
CCAAGCAATCGCCATCGTCGGTGATGGAGGCTTTTCCATGGTCATGCAAGATTTCGTCACTGCAGTAGGTTTAAACATGC
CAATGATTGTCGTCGTGTTAAACAACCAACAACTTTCATTCATTAAATATGAACAACAATCAGCTGGTGAACTAAACTAC
GCCATCGATCTACCAGACATCAATTACGCAAAATTCGCCGAAAGTTGCGGAGGAATTGGTTTCCGAGTAGAAAAAATGGC
GGATCTAGAAGCTGCTTTCGAAAATGCTAAACTAGCAACTAAGCCAGTAATCATCGACGTTTCCGTGGACAGTGCAGCCG
CTCCACTACCTGGTAAAATCGTGATGGACGAAGCACTCGGTTATACTAAATTCGAAATCCAATCAGTCTTAGAAGACCAT
CGTTTCGCAAAAATGCCACCACTTAAAACCATTTTACGCAGATTTTTATAA

Upstream 100 bases:

>100_bases
GAATAGTTCGTCAATTCTCTTTTATGAATACTCTTTTAAAAACAGGGTAAACGAAAACTATTGGACATTAGAGAAAACAT
CCATGGGAGGAGAAAGTAAA

Downstream 100 bases:

>100_bases
AAAATAAATAAAAAAATAACATTATCACCCGTTTCATGCCGATTTTCGCTAATTATTATAACGTTTTTTTATTAAAACTA
ATTATTTTACAATAAAATAT

Product: pyruvate oxidase

Products: acetyl phosphate; CO2; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MKKVKASETLVQTLKNWGIDHVYGLPGDSIDTVVDALRKEQEAIEFIHVRHEEVATLAAAAYTKLTGKIGVALSIGGPGA
IHLLNGMYDAKMDHVPMLVLAGQVTTDVLNTGFFQEVNLPAIFEDVAVYNKQIDNAETLADVVDEAIRTAYKEKGVAVLT
IPNDIPSQVIKASLEAKPVKFEQENPKLDEAAIQEAVARIEKAEKPVILAGLGTKHAGPELIAFSEKLKIPIIHSLPAKT
IVPDNHPNALGNLGKIGTKPAYEAMQETDLLLMFGNDYPYSDYLPKKADCIQIDIDPAKISKRFPATVGLVGDAAEIIGN
LTAKTAPVEERKFLQACQENMQEWWKWLEEDIAQTTDPIAPEVVMANIQKIADKDAIFSIDVGTATVWSTRYLHLAPQND
FIVSAWLGTMGCGLPGAIAAKKAFPDRQAIAIVGDGGFSMVMQDFVTAVGLNMPMIVVVLNNQQLSFIKYEQQSAGELNY
AIDLPDINYAKFAESCGGIGFRVEKMADLEAAFENAKLATKPVIIDVSVDSAAAPLPGKIVMDEALGYTKFEIQSVLEDH
RFAKMPPLKTILRRFL

Sequences:

>Translated_576_residues
MKKVKASETLVQTLKNWGIDHVYGLPGDSIDTVVDALRKEQEAIEFIHVRHEEVATLAAAAYTKLTGKIGVALSIGGPGA
IHLLNGMYDAKMDHVPMLVLAGQVTTDVLNTGFFQEVNLPAIFEDVAVYNKQIDNAETLADVVDEAIRTAYKEKGVAVLT
IPNDIPSQVIKASLEAKPVKFEQENPKLDEAAIQEAVARIEKAEKPVILAGLGTKHAGPELIAFSEKLKIPIIHSLPAKT
IVPDNHPNALGNLGKIGTKPAYEAMQETDLLLMFGNDYPYSDYLPKKADCIQIDIDPAKISKRFPATVGLVGDAAEIIGN
LTAKTAPVEERKFLQACQENMQEWWKWLEEDIAQTTDPIAPEVVMANIQKIADKDAIFSIDVGTATVWSTRYLHLAPQND
FIVSAWLGTMGCGLPGAIAAKKAFPDRQAIAIVGDGGFSMVMQDFVTAVGLNMPMIVVVLNNQQLSFIKYEQQSAGELNY
AIDLPDINYAKFAESCGGIGFRVEKMADLEAAFENAKLATKPVIIDVSVDSAAAPLPGKIVMDEALGYTKFEIQSVLEDH
RFAKMPPLKTILRRFL
>Mature_576_residues
MKKVKASETLVQTLKNWGIDHVYGLPGDSIDTVVDALRKEQEAIEFIHVRHEEVATLAAAAYTKLTGKIGVALSIGGPGA
IHLLNGMYDAKMDHVPMLVLAGQVTTDVLNTGFFQEVNLPAIFEDVAVYNKQIDNAETLADVVDEAIRTAYKEKGVAVLT
IPNDIPSQVIKASLEAKPVKFEQENPKLDEAAIQEAVARIEKAEKPVILAGLGTKHAGPELIAFSEKLKIPIIHSLPAKT
IVPDNHPNALGNLGKIGTKPAYEAMQETDLLLMFGNDYPYSDYLPKKADCIQIDIDPAKISKRFPATVGLVGDAAEIIGN
LTAKTAPVEERKFLQACQENMQEWWKWLEEDIAQTTDPIAPEVVMANIQKIADKDAIFSIDVGTATVWSTRYLHLAPQND
FIVSAWLGTMGCGLPGAIAAKKAFPDRQAIAIVGDGGFSMVMQDFVTAVGLNMPMIVVVLNNQQLSFIKYEQQSAGELNY
AIDLPDINYAKFAESCGGIGFRVEKMADLEAAFENAKLATKPVIIDVSVDSAAAPLPGKIVMDEALGYTKFEIQSVLEDH
RFAKMPPLKTILRRFL

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=576, Percent_Identity=24.6527777777778, Blast_Score=156, Evalue=5e-38,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=31.9642857142857, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=29.2682926829268, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1790104, Length=539, Percent_Identity=28.3858998144712, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1786717, Length=562, Percent_Identity=25.9786476868327, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1788716, Length=560, Percent_Identity=26.4285714285714, Blast_Score=135, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=556, Percent_Identity=23.5611510791367, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17531301, Length=556, Percent_Identity=23.5611510791367, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17542570, Length=557, Percent_Identity=23.1597845601436, Blast_Score=102, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=572, Percent_Identity=27.972027972028, Blast_Score=206, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6320816, Length=542, Percent_Identity=23.4317343173432, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320588, Length=577, Percent_Identity=20.103986135182, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320123, Length=540, Percent_Identity=21.1111111111111, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321524, Length=513, Percent_Identity=21.2475633528265, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922626, Length=587, Percent_Identity=25.2129471890971, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.3.3

Molecular weight: Translated: 62749; Mature: 62749

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKASETLVQTLKNWGIDHVYGLPGDSIDTVVDALRKEQEAIEFIHVRHEEVATLAAA
CCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYTKLTGKIGVALSIGGPGAIHLLNGMYDAKMDHVPMLVLAGQVTTDVLNTGFFQEVNLP
HHHHHCCCEEEEEEECCCCHHHHHCCHHHCCCCCCCEEEEECHHHHHHHHCCCCEECCCC
AIFEDVAVYNKQIDNAETLADVVDEAIRTAYKEKGVAVLTIPNDIPSQVIKASLEAKPVK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCE
FEQENPKLDEAAIQEAVARIEKAEKPVILAGLGTKHAGPELIAFSEKLKIPIIHSLPAKT
ECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCEEECCCCCC
IVPDNHPNALGNLGKIGTKPAYEAMQETDLLLMFGNDYPYSDYLPKKADCIQIDIDPAKI
CCCCCCCCHHCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCHHHH
SKRFPATVGLVGDAAEIIGNLTAKTAPVEERKFLQACQENMQEWWKWLEEDIAQTTDPIA
HCCCCCEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PEVVMANIQKIADKDAIFSIDVGTATVWSTRYLHLAPQNDFIVSAWLGTMGCGLPGAIAA
HHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEEEECCCCCEEEEEHHHHHCCCCCCHHHH
KKAFPDRQAIAIVGDGGFSMVMQDFVTAVGLNMPMIVVVLNNQQLSFIKYEQQSAGELNY
HHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCEEE
AIDLPDINYAKFAESCGGIGFRVEKMADLEAAFENAKLATKPVIIDVSVDSAAAPLPGKI
EEECCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCE
VMDEALGYTKFEIQSVLEDHRFAKMPPLKTILRRFL
EEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHC
>Mature Secondary Structure
MKKVKASETLVQTLKNWGIDHVYGLPGDSIDTVVDALRKEQEAIEFIHVRHEEVATLAAA
CCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYTKLTGKIGVALSIGGPGAIHLLNGMYDAKMDHVPMLVLAGQVTTDVLNTGFFQEVNLP
HHHHHCCCEEEEEEECCCCHHHHHCCHHHCCCCCCCEEEEECHHHHHHHHCCCCEECCCC
AIFEDVAVYNKQIDNAETLADVVDEAIRTAYKEKGVAVLTIPNDIPSQVIKASLEAKPVK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCE
FEQENPKLDEAAIQEAVARIEKAEKPVILAGLGTKHAGPELIAFSEKLKIPIIHSLPAKT
ECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCEEECCCCCC
IVPDNHPNALGNLGKIGTKPAYEAMQETDLLLMFGNDYPYSDYLPKKADCIQIDIDPAKI
CCCCCCCCHHCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEECCHHHH
SKRFPATVGLVGDAAEIIGNLTAKTAPVEERKFLQACQENMQEWWKWLEEDIAQTTDPIA
HCCCCCEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PEVVMANIQKIADKDAIFSIDVGTATVWSTRYLHLAPQNDFIVSAWLGTMGCGLPGAIAA
HHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEEEECCCCCEEEEEHHHHHCCCCCCHHHH
KKAFPDRQAIAIVGDGGFSMVMQDFVTAVGLNMPMIVVVLNNQQLSFIKYEQQSAGELNY
HHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCEEE
AIDLPDINYAKFAESCGGIGFRVEKMADLEAAFENAKLATKPVIIDVSVDSAAAPLPGKI
EEECCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCE
VMDEALGYTKFEIQSVLEDHRFAKMPPLKTILRRFL
EEHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; phosphate; O2; H2O

Specific reaction: pyruvate + phosphate + O2 + H2O = acetyl phosphate + CO2 + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]