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The map label for this gene is ywpJ [H]

Identifier: 226223293

GI number: 226223293

Start: 710573

End: 711439

Strand: Reverse

Name: ywpJ [H]

Synonym: Lm4b_00690

Alternate gene names: 226223293

Gene position: 711439-710573 (Counterclockwise)

Preceding gene: 226223302

Following gene: 226223292

Centisome position: 24.43

GC content: 38.06

Gene sequence:

>867_bases
ATGATTAAAGTTATTGCTTCAGACATGGATGGCACACTACTTAACTCTGATATCGAGATTGCACAAGAAAACGTCGAGGC
AATCGAAAAAGCCCGCGCCAAAGGAATTCATTTCGTTCTTTGCACCGGACGTATGTATGACGATGCAATGGGGTTAATTA
ATAAAGCTAATTTATATGCACCAGCAATTTGTATGAATGGTGCGGAAATCCGCGATGAACACGGCAAAATAATTTTGCAA
CATCCGATTGATAGAAACCTAGCAAGAAACACTTACAACACGCTGACTGAATTAGGTATGTATACCGAATTTTTCACTGA
CATGGGCCCAATCACAACAGACAAAGCTCGTGGCAAAGAATTTATGATTGAAATGCACAAACGCATCCACCCAGACGTTC
CCGCAGAAACCATTATGGACAAAGTAGAAGAACGTTTTGAATCGAAGGACGTTCACGAAATTCCAGATGTAGAGCGTATT
TTAGGCAATAAAGATTTAACCATTCTAAAATTCATTTCCTTCTCTTCTGATAAAGACATTCTTGCAAAAGCCAAACAAAA
ACTAGAAAAAGATTCCGAACTATCCGTTACATCTTCTTTCTCTGATAATATTGAAATCACTCACCGCGAAGCACAAAAAG
GTATTTCTTTACAATATTACGTAGAAAAATTAGGCGTAACACTTGATGAAACTTTTGCAATTGGAGATAATATGAATGAT
ATTTCGATGCTAAAAATGGCTGGTTATAGTGTAGCTATGGGTAACGGCGAAGAAGAAGTCAAAGATCTTGCAGAACACGT
AACCCTTTCGAATGATGAGCACGGCGTTGCAGCAGCCATCTATAAAATGTTAGAAACAAATGACTAA

Upstream 100 bases:

>100_bases
CGCTCCACCTAAAAAACTACGCGCAGCTACCTTTTGTGCATTTTTCTTAAAAAAATGATATTCTAGTGGAGTAGGTAAAA
TTGGGGAGGAATTTAATATT

Downstream 100 bases:

>100_bases
AGTTTATTTATAGGAGGAAACACGAATGACAATCAAAAAAACATTAACTATTGCAGGTTCTGATTCAAGTGGTGGTGCTG
GTTTACAAGCAGACTTAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MIKVIASDMDGTLLNSDIEIAQENVEAIEKARAKGIHFVLCTGRMYDDAMGLINKANLYAPAICMNGAEIRDEHGKIILQ
HPIDRNLARNTYNTLTELGMYTEFFTDMGPITTDKARGKEFMIEMHKRIHPDVPAETIMDKVEERFESKDVHEIPDVERI
LGNKDLTILKFISFSSDKDILAKAKQKLEKDSELSVTSSFSDNIEITHREAQKGISLQYYVEKLGVTLDETFAIGDNMND
ISMLKMAGYSVAMGNGEEEVKDLAEHVTLSNDEHGVAAAIYKMLETND

Sequences:

>Translated_288_residues
MIKVIASDMDGTLLNSDIEIAQENVEAIEKARAKGIHFVLCTGRMYDDAMGLINKANLYAPAICMNGAEIRDEHGKIILQ
HPIDRNLARNTYNTLTELGMYTEFFTDMGPITTDKARGKEFMIEMHKRIHPDVPAETIMDKVEERFESKDVHEIPDVERI
LGNKDLTILKFISFSSDKDILAKAKQKLEKDSELSVTSSFSDNIEITHREAQKGISLQYYVEKLGVTLDETFAIGDNMND
ISMLKMAGYSVAMGNGEEEVKDLAEHVTLSNDEHGVAAAIYKMLETND
>Mature_288_residues
MIKVIASDMDGTLLNSDIEIAQENVEAIEKARAKGIHFVLCTGRMYDDAMGLINKANLYAPAICMNGAEIRDEHGKIILQ
HPIDRNLARNTYNTLTELGMYTEFFTDMGPITTDKARGKEFMIEMHKRIHPDVPAETIMDKVEERFESKDVHEIPDVERI
LGNKDLTILKFISFSSDKDILAKAKQKLEKDSELSVTSSFSDNIEITHREAQKGISLQYYVEKLGVTLDETFAIGDNMND
ISMLKMAGYSVAMGNGEEEVKDLAEHVTLSNDEHGVAAAIYKMLETND

Specific function: Putative phosphatase (Potential) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI1786982, Length=286, Percent_Identity=28.6713286713287, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI48994981, Length=265, Percent_Identity=29.0566037735849, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI2367265, Length=287, Percent_Identity=29.616724738676, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787043, Length=295, Percent_Identity=25.7627118644068, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 32313; Mature: 32313

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVIASDMDGTLLNSDIEIAQENVEAIEKARAKGIHFVLCTGRMYDDAMGLINKANLYA
CEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHCCCCC
PAICMNGAEIRDEHGKIILQHPIDRNLARNTYNTLTELGMYTEFFTDMGPITTDKARGKE
CHHCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHH
FMIEMHKRIHPDVPAETIMDKVEERFESKDVHEIPDVERILGNKDLTILKFISFSSDKDI
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCHHH
LAKAKQKLEKDSELSVTSSFSDNIEITHREAQKGISLQYYVEKLGVTLDETFAIGDNMND
HHHHHHHHCCCCCCEEECCCCCCEEEEEHHHHCCCCHHHHHHHHCCCHHHEEECCCCCCH
ISMLKMAGYSVAMGNGEEEVKDLAEHVTLSNDEHGVAAAIYKMLETND
HHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKVIASDMDGTLLNSDIEIAQENVEAIEKARAKGIHFVLCTGRMYDDAMGLINKANLYA
CEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHCCCCC
PAICMNGAEIRDEHGKIILQHPIDRNLARNTYNTLTELGMYTEFFTDMGPITTDKARGKE
CHHCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHH
FMIEMHKRIHPDVPAETIMDKVEERFESKDVHEIPDVERILGNKDLTILKFISFSSDKDI
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCHHH
LAKAKQKLEKDSELSVTSSFSDNIEITHREAQKGISLQYYVEKLGVTLDETFAIGDNMND
HHHHHHHHCCCCCCEEECCCCCCEEEEEHHHHCCCCHHHHHHHHCCCHHHEEECCCCCCH
ISMLKMAGYSVAMGNGEEEVKDLAEHVTLSNDEHGVAAAIYKMLETND
HHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]