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The map label for this gene is thiD

Identifier: 226223292

GI number: 226223292

Start: 709732

End: 710547

Strand: Reverse

Name: thiD

Synonym: Lm4b_00689

Alternate gene names: 226223292

Gene position: 710547-709732 (Counterclockwise)

Preceding gene: 226223293

Following gene: 226223291

Centisome position: 24.39

GC content: 40.56

Gene sequence:

>816_bases
ATGACAATCAAAAAAACATTAACTATTGCAGGTTCTGATTCAAGTGGTGGTGCTGGTTTACAAGCAGACTTAAAAACGTT
TGAAGAATACGGCACTTATGGTTTTAGCGCAATCACAACAATCGTAACAATGGATCCAGATAACAACTGGGCGCACGGCG
TTACTCCAATTGATGCGCAACTTGTTCGCGAGCAACTTAAAACAATCCTTTCTGGTGGTCCTGTTGACGCAATGAAAACA
GGTATGCTCGGTTCAATTGACATCATTAAAGCTACACGCGAAGCCATCGACAAATATGATTTAAAAAATGTCGTAATCGA
TCCAGTTATGGTTTGTAAAGGGGAAGACGAATTAATCCAACCAGAAAACGCGGAAGCTATCCGCGACCTACTGCTTCCAA
AAGCAACAATCACAACACCAAACCTATTCGAAGCAGGTCAATTATCCGGTCTTGGCAAATTAACTACATTAGATGATATG
AAAAAAGCAGCGAAAAAAATCATCGAATTAGGTGCTAAATACGTAGTTATCAAAGGCGGAAAAGCCCTAGAAAGCGACAA
AGCAATTGACCTACTATATGATGGCAAAGAATTTACTATCTATGAAGTAGAAAAAATCTCTCCAAGCCACAATCACGGCG
CTGGCTGTACTTTCGCAGCAGCAATCACAGCTGGACTTGCAAAAGGTTTAACGGTTGAAGAAGCAGTCGCAAAAGCAAAA
GACTATGTCACTGCAGCTATCAAAGGTGGATTTGCACTAAACGAATTCATCGGTCCTGTTTGGCATGGCGCTTATAATAA
AGCAGAAAATAGATAA

Upstream 100 bases:

>100_bases
GAACACGTAACCCTTTCGAATGATGAGCACGGCGTTGCAGCAGCCATCTATAAAATGTTAGAAACAAATGACTAAAGTTT
ATTTATAGGAGGAAACACGA

Downstream 100 bases:

>100_bases
TACGAAAACCGTCCACTGTTTGGGCGGTTTTTTATTATAATAAAAGTTAGGAGATGAGTTCAATGAAAGTAAGTAAATCA
TTTGAAGCGTTCGCGAAAGA

Product: phosphomethylpyrimidine kinase thiD

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTIKKTLTIAGSDSSGGAGLQADLKTFEEYGTYGFSAITTIVTMDPDNNWAHGVTPIDAQLVREQLKTILSGGPVDAMKT
GMLGSIDIIKATREAIDKYDLKNVVIDPVMVCKGEDELIQPENAEAIRDLLLPKATITTPNLFEAGQLSGLGKLTTLDDM
KKAAKKIIELGAKYVVIKGGKALESDKAIDLLYDGKEFTIYEVEKISPSHNHGAGCTFAAAITAGLAKGLTVEEAVAKAK
DYVTAAIKGGFALNEFIGPVWHGAYNKAENR

Sequences:

>Translated_271_residues
MTIKKTLTIAGSDSSGGAGLQADLKTFEEYGTYGFSAITTIVTMDPDNNWAHGVTPIDAQLVREQLKTILSGGPVDAMKT
GMLGSIDIIKATREAIDKYDLKNVVIDPVMVCKGEDELIQPENAEAIRDLLLPKATITTPNLFEAGQLSGLGKLTTLDDM
KKAAKKIIELGAKYVVIKGGKALESDKAIDLLYDGKEFTIYEVEKISPSHNHGAGCTFAAAITAGLAKGLTVEEAVAKAK
DYVTAAIKGGFALNEFIGPVWHGAYNKAENR
>Mature_270_residues
TIKKTLTIAGSDSSGGAGLQADLKTFEEYGTYGFSAITTIVTMDPDNNWAHGVTPIDAQLVREQLKTILSGGPVDAMKTG
MLGSIDIIKATREAIDKYDLKNVVIDPVMVCKGEDELIQPENAEAIRDLLLPKATITTPNLFEAGQLSGLGKLTTLDDMK
KAAKKIIELGAKYVVIKGGKALESDKAIDLLYDGKEFTIYEVEKISPSHNHGAGCTFAAAITAGLAKGLTVEEAVAKAKD
YVTAAIKGGFALNEFIGPVWHGAYNKAENR

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates. Can also use hydroxymethylpyrimidine (HMP) as substrate [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=258, Percent_Identity=35.2713178294574, Blast_Score=157, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6325378, Length=269, Percent_Identity=30.1115241635688, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324997, Length=254, Percent_Identity=30.7086614173228, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324517, Length=254, Percent_Identity=30.3149606299213, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 28878; Mature: 28747

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKKTLTIAGSDSSGGAGLQADLKTFEEYGTYGFSAITTIVTMDPDNNWAHGVTPIDAQ
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCHHHH
LVREQLKTILSGGPVDAMKTGMLGSIDIIKATREAIDKYDLKNVVIDPVMVCKGEDELIQ
HHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
PENAEAIRDLLLPKATITTPNLFEAGQLSGLGKLTTLDDMKKAAKKIIELGAKYVVIKGG
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KALESDKAIDLLYDGKEFTIYEVEKISPSHNHGAGCTFAAAITAGLAKGLTVEEAVAKAK
CCCCCCCCEEEEECCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
DYVTAAIKGGFALNEFIGPVWHGAYNKAENR
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TIKKTLTIAGSDSSGGAGLQADLKTFEEYGTYGFSAITTIVTMDPDNNWAHGVTPIDAQ
CCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCHHHH
LVREQLKTILSGGPVDAMKTGMLGSIDIIKATREAIDKYDLKNVVIDPVMVCKGEDELIQ
HHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
PENAEAIRDLLLPKATITTPNLFEAGQLSGLGKLTTLDDMKKAAKKIIELGAKYVVIKGG
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
KALESDKAIDLLYDGKEFTIYEVEKISPSHNHGAGCTFAAAITAGLAKGLTVEEAVAKAK
CCCCCCCCEEEEECCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
DYVTAAIKGGFALNEFIGPVWHGAYNKAENR
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]