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The map label for this gene is pbuG [H]

Identifier: 226223202

GI number: 226223202

Start: 613277

End: 614572

Strand: Reverse

Name: pbuG [H]

Synonym: Lm4b_00599

Alternate gene names: 226223202

Gene position: 614572-613277 (Counterclockwise)

Preceding gene: 226223206

Following gene: 226223200

Centisome position: 21.1

GC content: 41.74

Gene sequence:

>1296_bases
ATGGATAAATTTTTCAAACTACGCGAGAACAAGACGACTGTTCGAACAGAAATACTCGCGGGGCTAACGACTTTCTTATC
GATGGCTTATGTACTTTTCGTCAACCCATCTATGCTTGCAACAACTGGAATGGAATTAAAAGCTGTTTTTGTGGCGACGA
TTCTTGCTTCTGTTGTAGGATCGCTTGCAATGGGACTAATCGCTAATTACCCGATTGGACTTGCACCAGGTATGGGACTA
AATGCGTTTTTCGCTTATACCGTTTGTGCGCAGTGGGGAATTCCTTGGCAAACGGCGCTGGCTGGTGTTCTCGTTTCTGG
CTTAGTGTTCATTTGTTTAACTATTTCTGGACTTCGCGAAAAAATCGTTAATGCAATCCCAACGGAACTAAAATTCGCAG
TGGGAGCAGGTATTGGTTTTTTTATCGCCTTTTTAGGGCTTAAAAATGCTGGAATTATTGTGCCAAATGAATCTACTATT
GTAGCGCTTGGTGACTTACATTCTGGCCCAGTGTTACTGGCTGTTTTCGGGATTGTTGTTACTGTTGCTTACATGACAAT
TGGTTGGAAAGGTGCGATTTTCTTTGGAATGGCAACAACCGCTGTTGCTGGAATGTTGTTTGGACTGATTGATGTGCCGA
CTCAAGTTGTTTCATCTGTACCAAGTATGGAGTCAACGTTTGGTCAAGCAATTATTCACTTACCAGATATTTTTACACCA
CAAATGCTCATTGTTATTTTGACATTTTTCTTCATTGATTTTTTTGATACTGCCGGGACACTTGTTGCGGTTGCGACTCA
AGCTGGATTTGTAAAAGATAACAAAATTCCGCGCGCAGGTCGTTCGCTATTCTCGGATTCACTTGCAACAGTATTTGGAG
CGATTTTCGGGACTTCAACAACGACTTCTTATGTAGAATCTACTGCTGGGGTTGCTGTTGGTGGTCGGACAGGTTTAACT
GCCGTTGTTATCGCAATCTGTTTCTCACTGTCACTATTCTTCTCGCCGCTTCTTGGCGTAATTACAAGTGCGGTTACGAC
TCCAGCGCTTGTCATTGTTGGGATTTTAATGATTGGAAATGTCGCTCATATTGATTGGACTAAATTCGAAGTAGCTGTTC
CGTCCTTTTTCGTTATTTTAATGATGGTACTGACTTTCTCTATCGCTACTGGTATCGCAATTGGTTTCATTTTCTATCCA
ATCACCATGGTACTTAAAGGACGCTACAAAGAAGTGCATCCGATTATGTACGTGATGATGGTACTGTTTATACTGTACTT
TATGTTCGTCGTTTAG

Upstream 100 bases:

>100_bases
AATTTAATAGGGATTTTTTTGCTATTTTTATTTATTATTCCCTTTTTTACCTAGGCTCTTTCCGTTATGTGCGACAACAT
AAATAGGGAGGCTTTTTTTA

Downstream 100 bases:

>100_bases
ACAGCAAAAAAACCGGGGATTCCGCATCCTCGGTTTTTTCGTTTAATCATAATACACAATCATATAGTTTTTGCCTTTTT
CCTCGTCGTATCCTTGTACA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MDKFFKLRENKTTVRTEILAGLTTFLSMAYVLFVNPSMLATTGMELKAVFVATILASVVGSLAMGLIANYPIGLAPGMGL
NAFFAYTVCAQWGIPWQTALAGVLVSGLVFICLTISGLREKIVNAIPTELKFAVGAGIGFFIAFLGLKNAGIIVPNESTI
VALGDLHSGPVLLAVFGIVVTVAYMTIGWKGAIFFGMATTAVAGMLFGLIDVPTQVVSSVPSMESTFGQAIIHLPDIFTP
QMLIVILTFFFIDFFDTAGTLVAVATQAGFVKDNKIPRAGRSLFSDSLATVFGAIFGTSTTTSYVESTAGVAVGGRTGLT
AVVIAICFSLSLFFSPLLGVITSAVTTPALVIVGILMIGNVAHIDWTKFEVAVPSFFVILMMVLTFSIATGIAIGFIFYP
ITMVLKGRYKEVHPIMYVMMVLFILYFMFVV

Sequences:

>Translated_431_residues
MDKFFKLRENKTTVRTEILAGLTTFLSMAYVLFVNPSMLATTGMELKAVFVATILASVVGSLAMGLIANYPIGLAPGMGL
NAFFAYTVCAQWGIPWQTALAGVLVSGLVFICLTISGLREKIVNAIPTELKFAVGAGIGFFIAFLGLKNAGIIVPNESTI
VALGDLHSGPVLLAVFGIVVTVAYMTIGWKGAIFFGMATTAVAGMLFGLIDVPTQVVSSVPSMESTFGQAIIHLPDIFTP
QMLIVILTFFFIDFFDTAGTLVAVATQAGFVKDNKIPRAGRSLFSDSLATVFGAIFGTSTTTSYVESTAGVAVGGRTGLT
AVVIAICFSLSLFFSPLLGVITSAVTTPALVIVGILMIGNVAHIDWTKFEVAVPSFFVILMMVLTFSIATGIAIGFIFYP
ITMVLKGRYKEVHPIMYVMMVLFILYFMFVV
>Mature_431_residues
MDKFFKLRENKTTVRTEILAGLTTFLSMAYVLFVNPSMLATTGMELKAVFVATILASVVGSLAMGLIANYPIGLAPGMGL
NAFFAYTVCAQWGIPWQTALAGVLVSGLVFICLTISGLREKIVNAIPTELKFAVGAGIGFFIAFLGLKNAGIIVPNESTI
VALGDLHSGPVLLAVFGIVVTVAYMTIGWKGAIFFGMATTAVAGMLFGLIDVPTQVVSSVPSMESTFGQAIIHLPDIFTP
QMLIVILTFFFIDFFDTAGTLVAVATQAGFVKDNKIPRAGRSLFSDSLATVFGAIFGTSTTTSYVESTAGVAVGGRTGLT
AVVIAICFSLSLFFSPLLGVITSAVTTPALVIVGILMIGNVAHIDWTKFEVAVPSFFVILMMVLTFSIATGIAIGFIFYP
ITMVLKGRYKEVHPIMYVMMVLFILYFMFVV

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=432, Percent_Identity=41.6666666666667, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1790150, Length=431, Percent_Identity=40.1392111368909, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI1790499, Length=433, Percent_Identity=34.4110854503464, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI48994909, Length=419, Percent_Identity=35.3221957040573, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45874; Mature: 45874

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFFKLRENKTTVRTEILAGLTTFLSMAYVLFVNPSMLATTGMELKAVFVATILASVVG
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHH
SLAMGLIANYPIGLAPGMGLNAFFAYTVCAQWGIPWQTALAGVLVSGLVFICLTISGLRE
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KIVNAIPTELKFAVGAGIGFFIAFLGLKNAGIIVPNESTIVALGDLHSGPVLLAVFGIVV
HHHHHCCHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHH
TVAYMTIGWKGAIFFGMATTAVAGMLFGLIDVPTQVVSSVPSMESTFGQAIIHLPDIFTP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCH
QMLIVILTFFFIDFFDTAGTLVAVATQAGFVKDNKIPRAGRSLFSDSLATVFGAIFGTST
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TTSYVESTAGVAVGGRTGLTAVVIAICFSLSLFFSPLLGVITSAVTTPALVIVGILMIGN
HHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VAHIDWTKFEVAVPSFFVILMMVLTFSIATGIAIGFIFYPITMVLKGRYKEVHPIMYVMM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VLFILYFMFVV
HHHHHHHHHHC
>Mature Secondary Structure
MDKFFKLRENKTTVRTEILAGLTTFLSMAYVLFVNPSMLATTGMELKAVFVATILASVVG
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHH
SLAMGLIANYPIGLAPGMGLNAFFAYTVCAQWGIPWQTALAGVLVSGLVFICLTISGLRE
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KIVNAIPTELKFAVGAGIGFFIAFLGLKNAGIIVPNESTIVALGDLHSGPVLLAVFGIVV
HHHHHCCHHHHHHHHCCHHHHHHHHHCCCCCEEECCCCCEEEECCCCCCHHHHHHHHHHH
TVAYMTIGWKGAIFFGMATTAVAGMLFGLIDVPTQVVSSVPSMESTFGQAIIHLPDIFTP
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCH
QMLIVILTFFFIDFFDTAGTLVAVATQAGFVKDNKIPRAGRSLFSDSLATVFGAIFGTST
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TTSYVESTAGVAVGGRTGLTAVVIAICFSLSLFFSPLLGVITSAVTTPALVIVGILMIGN
HHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VAHIDWTKFEVAVPSFFVILMMVLTFSIATGIAIGFIFYPITMVLKGRYKEVHPIMYVMM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VLFILYFMFVV
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377; 11591660 [H]