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The map label for this gene is ybaZ [H]

Identifier: 226223200

GI number: 226223200

Start: 611830

End: 612126

Strand: Reverse

Name: ybaZ [H]

Synonym: Lm4b_00597

Alternate gene names: 226223200

Gene position: 612126-611830 (Counterclockwise)

Preceding gene: 226223202

Following gene: 226223198

Centisome position: 21.02

GC content: 45.12

Gene sequence:

>297_bases
ATGATTCCAGCAGATTTTGAAGACCGTGTTTATACAGTCGTGCGCCAAATACCGCGCGGTAAAGTGACTACATATGGCCA
AATCGCCTATATGATTGGCTTTCCTAAAAACGCCAGACTCGTAGGTGCTACGCTAAAACATTCGAAGCGTGACCAAATTA
CGCCTTGCCATCGTGTCGTCAATGCAGCTGGTCGACTTGTCCCCGGTTGGGAAGAGCAACGCGAACTCCTTTTATCAGAA
GGTGTGACATTTAAAGAGAATGGACACGTCAAAATGAAAGATCATTTTTGGCAATAA

Upstream 100 bases:

>100_bases
TTTTGCGTATGAAAAATCTGGCATTCCTTTATCATCCTAACTTTTATAAAATAGGTATTTCCTGTATACTAAATTTATTA
TGAACAAATGGAGGTACGTA

Downstream 100 bases:

>100_bases
GTGCTATATCCACGTCCTATATCTTGAACACCGTGCGAGTCCGACCCATAGACAAACGGAATCTGTAACTCGCTAGCTAA
TGTCACTATTTTCTTTGGTG

Product: methyltransferase

Products: DNA; protein S-methyl-L-cysteine

Alternate protein names: NA

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
MIPADFEDRVYTVVRQIPRGKVTTYGQIAYMIGFPKNARLVGATLKHSKRDQITPCHRVVNAAGRLVPGWEEQRELLLSE
GVTFKENGHVKMKDHFWQ

Sequences:

>Translated_98_residues
MIPADFEDRVYTVVRQIPRGKVTTYGQIAYMIGFPKNARLVGATLKHSKRDQITPCHRVVNAAGRLVPGWEEQRELLLSE
GVTFKENGHVKMKDHFWQ
>Mature_98_residues
MIPADFEDRVYTVVRQIPRGKVTTYGQIAYMIGFPKNARLVGATLKHSKRDQITPCHRVVNAAGRLVPGWEEQRELLLSE
GVTFKENGHVKMKDHFWQ

Specific function: Unknown

COG id: COG3695

COG function: function code L; Predicted methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1786659, Length=96, Percent_Identity=39.5833333333333, Blast_Score=73, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: 2.1.1.63

Molecular weight: Translated: 11264; Mature: 11264

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPADFEDRVYTVVRQIPRGKVTTYGQIAYMIGFPKNARLVGATLKHSKRDQITPCHRVV
CCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEHHHCCCCCCCCCHHHHHH
NAAGRLVPGWEEQRELLLSEGVTFKENGHVKMKDHFWQ
HHCCCCCCCHHHHHHHHHHCCCEECCCCCEEECCCCCC
>Mature Secondary Structure
MIPADFEDRVYTVVRQIPRGKVTTYGQIAYMIGFPKNARLVGATLKHSKRDQITPCHRVV
CCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEHHHCCCCCCCCCHHHHHH
NAAGRLVPGWEEQRELLLSEGVTFKENGHVKMKDHFWQ
HHCCCCCCCHHHHHHHHHHCCCEECCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing 6-O-methylguanine; protein L-cysteine

Specific reaction: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]