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The map label for this gene is srlE [H]

Identifier: 226223172

GI number: 226223172

Start: 585154

End: 586140

Strand: Reverse

Name: srlE [H]

Synonym: Lm4b_00569

Alternate gene names: 226223172

Gene position: 586140-585154 (Counterclockwise)

Preceding gene: 226223173

Following gene: 226223171

Centisome position: 20.12

GC content: 40.22

Gene sequence:

>987_bases
ATGTATCAATCAATTCATGTGAATAAAGGTCAAGGCGGTTGGGGAGTCGGACTAGATATTACTCCGAGCGAACCGAGAAC
AAAAGTTGTTTCTATTACTGGTGGTGGTATTCATCCTGTAGCACTGAAAATTGCCGAATTATCTGGAGCTGAAGCCGTGG
ATGGTTTTAAAAATTCAATTCCAGAAGAAGAAATGATGTGTGTTGTTATTGATTGTGGTGGCACTGCGAGAATTGGTGTT
TATCCGATGAAACGTATTCCAACAGTCGATGTGCTCCCTTCTTCTCCATCAGGTCCGCTCGCAAAACATATTACAGAAGA
TATTTTTGTTTCTGGCGTCAAACCTGAAGATATTGAAGTTCGAGACAGAAGCACTGAACCTGGGAAACCATTTGACAGAG
AGAAATTCAAAGAAGATTATGCCAAGATTAAAGAAGATCAAGAAGAAGCAAGCCAATTAAAAGAAGGTTTCCTTGTGAAA
TTTTCTCGTGGCATTGGTAAAGCAATGGGTGTGTTCTATCAAGCAGGGCGTGATTCTGTGGATATGCTTATTAAAAATAT
TATTCCGTTTATGGCTTTTATTAGTATGATTATCGGTATTATTAATTACACTGGGATTGGGAAATTAATTGCGAATACGC
TCTCTCCTTTAGCTGGATCACTCTGGGGAATGTTATTAATTGCTTTTATTTGTAGTTTGCCATTTCTTTCACCTATTCTC
GGTCCTGGAGCAGTTATCGCTCAAGTAATCGGCGTTCTGATTGGTTCTCAAATTGCGATTGGTGCTATTCCAGTTACTTA
CGCCTTACCAGCACTATTTGCAATTAATGCTCAAGCTGGTTGCGACTTTATCCCCGTAGGACTCTCTCTTGCAGAAGCTA
AGCCAAAAACGGTTCAGTTCGGCGTTCCAGCTATTCTTTATAGCCGGATGATTACTGGTGTCCTTGCTGTTGTTATCGCA
TGGGTATTCAGTATCGGTATGTATTAA

Upstream 100 bases:

>100_bases
CTGGAATCGTGGTTATATTAATGCGTGGTGTGATTACAGAATCAATTACGAAATTCTTAATGCGAAAAGAAAAAGTCTGA
AATAGAAGGAGGCAAATGAA

Downstream 100 bases:

>100_bases
TTTTAGGAGGAATTTGCTATGACTATAAGTAAAATTATGGAAATCGGGCCACTTGTCCCCGCTTTTGAAGAAGAAAAAAT
CGTTATCTTATTTGGACCAA

Product: PTS system, glucitol/sorbitol-specific enzyme IIBC component

Products: NA

Alternate protein names: EII-Gut; PTS system glucitol/sorbitol-specific EIIB component [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MYQSIHVNKGQGGWGVGLDITPSEPRTKVVSITGGGIHPVALKIAELSGAEAVDGFKNSIPEEEMMCVVIDCGGTARIGV
YPMKRIPTVDVLPSSPSGPLAKHITEDIFVSGVKPEDIEVRDRSTEPGKPFDREKFKEDYAKIKEDQEEASQLKEGFLVK
FSRGIGKAMGVFYQAGRDSVDMLIKNIIPFMAFISMIIGIINYTGIGKLIANTLSPLAGSLWGMLLIAFICSLPFLSPIL
GPGAVIAQVIGVLIGSQIAIGAIPVTYALPALFAINAQAGCDFIPVGLSLAEAKPKTVQFGVPAILYSRMITGVLAVVIA
WVFSIGMY

Sequences:

>Translated_328_residues
MYQSIHVNKGQGGWGVGLDITPSEPRTKVVSITGGGIHPVALKIAELSGAEAVDGFKNSIPEEEMMCVVIDCGGTARIGV
YPMKRIPTVDVLPSSPSGPLAKHITEDIFVSGVKPEDIEVRDRSTEPGKPFDREKFKEDYAKIKEDQEEASQLKEGFLVK
FSRGIGKAMGVFYQAGRDSVDMLIKNIIPFMAFISMIIGIINYTGIGKLIANTLSPLAGSLWGMLLIAFICSLPFLSPIL
GPGAVIAQVIGVLIGSQIAIGAIPVTYALPALFAINAQAGCDFIPVGLSLAEAKPKTVQFGVPAILYSRMITGVLAVVIA
WVFSIGMY
>Mature_328_residues
MYQSIHVNKGQGGWGVGLDITPSEPRTKVVSITGGGIHPVALKIAELSGAEAVDGFKNSIPEEEMMCVVIDCGGTARIGV
YPMKRIPTVDVLPSSPSGPLAKHITEDIFVSGVKPEDIEVRDRSTEPGKPFDREKFKEDYAKIKEDQEEASQLKEGFLVK
FSRGIGKAMGVFYQAGRDSVDMLIKNIIPFMAFISMIIGIINYTGIGKLIANTLSPLAGSLWGMLLIAFICSLPFLSPIL
GPGAVIAQVIGVLIGSQIAIGAIPVTYALPALFAINAQAGCDFIPVGLSLAEAKPKTVQFGVPAILYSRMITGVLAVVIA
WVFSIGMY

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3732

COG function: function code G; Phosphotransferase system sorbitol-specific component IIBC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994905, Length=325, Percent_Identity=51.0769230769231, Blast_Score=306, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011638
- InterPro:   IPR011618
- InterPro:   IPR004702 [H]

Pfam domain/function: PF07663 EIIBC-GUT_C; PF03612 EIIBC-GUT_N [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 34942; Mature: 34942

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS51102 PTS_EIIB_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQSIHVNKGQGGWGVGLDITPSEPRTKVVSITGGGIHPVALKIAELSGAEAVDGFKNSI
CCCCEEEECCCCCEECCEECCCCCCCCEEEEEECCCCCHHHHEEHHCCCCHHHHHHHHCC
PEEEMMCVVIDCGGTARIGVYPMKRIPTVDVLPSSPSGPLAKHITEDIFVSGVKPEDIEV
CCCCCEEEEEECCCCEEECCEEHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEE
RDRSTEPGKPFDREKFKEDYAKIKEDQEEASQLKEGFLVKFSRGIGKAMGVFYQAGRDSV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DMLIKNIIPFMAFISMIIGIINYTGIGKLIANTLSPLAGSLWGMLLIAFICSLPFLSPIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GPGAVIAQVIGVLIGSQIAIGAIPVTYALPALFAINAQAGCDFIPVGLSLAEAKPKTVQF
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHCCCCCEEEC
GVPAILYSRMITGVLAVVIAWVFSIGMY
CCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYQSIHVNKGQGGWGVGLDITPSEPRTKVVSITGGGIHPVALKIAELSGAEAVDGFKNSI
CCCCEEEECCCCCEECCEECCCCCCCCEEEEEECCCCCHHHHEEHHCCCCHHHHHHHHCC
PEEEMMCVVIDCGGTARIGVYPMKRIPTVDVLPSSPSGPLAKHITEDIFVSGVKPEDIEV
CCCCCEEEEEECCCCEEECCEEHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEE
RDRSTEPGKPFDREKFKEDYAKIKEDQEEASQLKEGFLVKFSRGIGKAMGVFYQAGRDSV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
DMLIKNIIPFMAFISMIIGIINYTGIGKLIANTLSPLAGSLWGMLLIAFICSLPFLSPIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GPGAVIAQVIGVLIGSQIAIGAIPVTYALPALFAINAQAGCDFIPVGLSLAEAKPKTVQF
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHCCCCCEEEC
GVPAILYSRMITGVLAVVIAWVFSIGMY
CCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9572925 [H]