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The map label for this gene is srlA [H]

Identifier: 226223173

GI number: 226223173

Start: 586161

End: 586682

Strand: Reverse

Name: srlA [H]

Synonym: Lm4b_00570

Alternate gene names: 226223173

Gene position: 586682-586161 (Counterclockwise)

Preceding gene: 226223174

Following gene: 226223172

Centisome position: 20.14

GC content: 39.08

Gene sequence:

>522_bases
GTGAATTATATTGAATGGTTTGGAACAAACTTTATCGGCCTATTTGAGGCTGGCGGCAAACAGTTTGTGAGTTTTATGAC
AGGAATTGTCCCTCTTTTAATTGTCCTTTTAACCTTTACTTACTCGATTATCGCCTTTATCGGGGAGGAGCGAGTCAACC
GAGCAATCCAATTTTCAGCCAAGAACATGATTTTGCGCTATACCTTAATGCCCGTTTTATCCGTACTGATGCTAACAAAT
CCAATGGCTTATACATTTGGTCGTTTTGTTAAAGAGCATCAGAAACCAGCTTTTTATGACTCCGTTGTATCGTTTTTGCA
CCCAGTTACAGGATTATTTCCATATGCGAATGCTGGTGAATTATTCGTTTATCTAGGCATTGCTAATGGTGTGGTCGAAG
CTGGATATAGTATGTCAAGCCTTGCAGTTCGTTACTTCCTAGCTGGAATCGTGGTTATATTAATGCGTGGTGTGATTACA
GAATCAATTACGAAATTCTTAATGCGAAAAGAAAAAGTCTGA

Upstream 100 bases:

>100_bases
TAGTAGTAAAAATAAGAATACAATCCAAGAAAAAGCAATTATTAAAACAGTCAACAGTTGCATGAAAGCGCTTTAAATAA
CTAAAAAGGAGTGTTTCCTA

Downstream 100 bases:

>100_bases
AATAGAAGGAGGCAAATGAAATGTATCAATCAATTCATGTGAATAAAGGTCAAGGCGGTTGGGGAGTCGGACTAGATATT
ACTCCGAGCGAACCGAGAAC

Product: PTS system, glucitol/sorbitol-specific enzyme II CII component

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate [C]

Alternate protein names: EIIC-Gut; PTS system glucitol/sorbitol-specific EIIC component [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MNYIEWFGTNFIGLFEAGGKQFVSFMTGIVPLLIVLLTFTYSIIAFIGEERVNRAIQFSAKNMILRYTLMPVLSVLMLTN
PMAYTFGRFVKEHQKPAFYDSVVSFLHPVTGLFPYANAGELFVYLGIANGVVEAGYSMSSLAVRYFLAGIVVILMRGVIT
ESITKFLMRKEKV

Sequences:

>Translated_173_residues
MNYIEWFGTNFIGLFEAGGKQFVSFMTGIVPLLIVLLTFTYSIIAFIGEERVNRAIQFSAKNMILRYTLMPVLSVLMLTN
PMAYTFGRFVKEHQKPAFYDSVVSFLHPVTGLFPYANAGELFVYLGIANGVVEAGYSMSSLAVRYFLAGIVVILMRGVIT
ESITKFLMRKEKV
>Mature_173_residues
MNYIEWFGTNFIGLFEAGGKQFVSFMTGIVPLLIVLLTFTYSIIAFIGEERVNRAIQFSAKNMILRYTLMPVLSVLMLTN
PMAYTFGRFVKEHQKPAFYDSVVSFLHPVTGLFPYANAGELFVYLGIANGVVEAGYSMSSLAVRYFLAGIVVILMRGVIT
ESITKFLMRKEKV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3730

COG function: function code G; Phosphotransferase system sorbitol-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994904, Length=166, Percent_Identity=42.7710843373494, Blast_Score=140, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004699 [H]

Pfam domain/function: PF03608 EII-GUT [H]

EC number: NA

Molecular weight: Translated: 19455; Mature: 19455

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS51107 PTS_EIIC_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYIEWFGTNFIGLFEAGGKQFVSFMTGIVPLLIVLLTFTYSIIAFIGEERVNRAIQFSA
CCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNMILRYTLMPVLSVLMLTNPMAYTFGRFVKEHQKPAFYDSVVSFLHPVTGLFPYANAGE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
LFVYLGIANGVVEAGYSMSSLAVRYFLAGIVVILMRGVITESITKFLMRKEKV
EEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYIEWFGTNFIGLFEAGGKQFVSFMTGIVPLLIVLLTFTYSIIAFIGEERVNRAIQFSA
CCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNMILRYTLMPVLSVLMLTNPMAYTFGRFVKEHQKPAFYDSVVSFLHPVTGLFPYANAGE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
LFVYLGIANGVVEAGYSMSSLAVRYFLAGIVVILMRGVITESITKFLMRKEKV
EEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; sorbitol [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9572925 [H]