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The map label for this gene is iolB [H]

Identifier: 226223009

GI number: 226223009

Start: 409526

End: 410347

Strand: Direct

Name: iolB [H]

Synonym: Lm4b_00402

Alternate gene names: 226223009

Gene position: 409526-410347 (Clockwise)

Preceding gene: 226223008

Following gene: 226223010

Centisome position: 14.06

GC content: 42.7

Gene sequence:

>822_bases
ATGGGCAAACTGTTACGAAAACCGTTAAATGAAAGAATAGCGCCTGGCATTACGTTTGTGCAGGATATTAACCAAGCTAA
TTCTCCGCTAAGTTATGTTGGTTTTCGCTTAATTGAAATGGAAAAAGGTGCTATTTATCAGGAGGAGCTTACTGATTTAG
AGTGTTGTATTGTTGCGCTTACTGGGAAAATTACAGTGAGTGAGGGTGACAATATTTTTGCGGAGATTGGGACAAGAGCG
AATGTCTTTGAAAAAATTCCGACAGATAGCGTGTTTATTTCTGGCGGACGGACGTTTCAAGTGAAGGCGGACACAGAAAA
AGCTCGTGTAGCGCTTTGCTACTCCCAGGCGAATCGAGACTTGCCAACGACACTTATTAAGGCTAGTGATAATTCGATTG
AGCAGCGCGGAAAATATCAAAATAAACGACTGGTACATAACATTTTGCCAGATGTAAGTGAGGTTGCGAGCAGTTTGTTG
GTTGTGGAAGTGTATACGGATGGCGGAAATTTTTCGAGTTATCCGCCGCATAAACATGACCGCGATAATCTGCCAGCGGA
GTCGCTTTTGGAAGAAAGTTATTATCATGAAATTAATCCGGAGCAAGGTTTTATTTTTCAACGTGTCTATACGGATGACC
GGACGCTTGATGAAACGATGGCTGTGGAGCATCAAAATGCGGTTATCGTTCCAGAAGGCTATCACCCAGTTGGCGTGCCA
GATGGATATGATTCATATTATTTGAACGTAATGGCTGGACCGAAACGGGTTTGGAAATTTCACAACGATCCGGACCATGA
ATGGATTTTAGAACGAGACTAA

Upstream 100 bases:

>100_bases
TCATGCTAACGGCAAAGACAGCGTGGATTTTTACACGCATAAGAAAGTTGTCACTGCGAGATATTCATTAAAAGGTTACG
AAGAATAGGAGGGCGCAAAA

Downstream 100 bases:

>100_bases
GAGGAGGATTTGGAGCATGAATCTAAACAAACATAGCGAACGAAAATTTGATTTAATCACAGTAGGACGCGCTTGTATTG
ATTTGAACGCAGTCGAATAC

Product: IolB protein

Products: NA

Alternate protein names: 5DG isomerase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MGKLLRKPLNERIAPGITFVQDINQANSPLSYVGFRLIEMEKGAIYQEELTDLECCIVALTGKITVSEGDNIFAEIGTRA
NVFEKIPTDSVFISGGRTFQVKADTEKARVALCYSQANRDLPTTLIKASDNSIEQRGKYQNKRLVHNILPDVSEVASSLL
VVEVYTDGGNFSSYPPHKHDRDNLPAESLLEESYYHEINPEQGFIFQRVYTDDRTLDETMAVEHQNAVIVPEGYHPVGVP
DGYDSYYLNVMAGPKRVWKFHNDPDHEWILERD

Sequences:

>Translated_273_residues
MGKLLRKPLNERIAPGITFVQDINQANSPLSYVGFRLIEMEKGAIYQEELTDLECCIVALTGKITVSEGDNIFAEIGTRA
NVFEKIPTDSVFISGGRTFQVKADTEKARVALCYSQANRDLPTTLIKASDNSIEQRGKYQNKRLVHNILPDVSEVASSLL
VVEVYTDGGNFSSYPPHKHDRDNLPAESLLEESYYHEINPEQGFIFQRVYTDDRTLDETMAVEHQNAVIVPEGYHPVGVP
DGYDSYYLNVMAGPKRVWKFHNDPDHEWILERD
>Mature_272_residues
GKLLRKPLNERIAPGITFVQDINQANSPLSYVGFRLIEMEKGAIYQEELTDLECCIVALTGKITVSEGDNIFAEIGTRAN
VFEKIPTDSVFISGGRTFQVKADTEKARVALCYSQANRDLPTTLIKASDNSIEQRGKYQNKRLVHNILPDVSEVASSLLV
VEVYTDGGNFSSYPPHKHDRDNLPAESLLEESYYHEINPEQGFIFQRVYTDDRTLDETMAVEHQNAVIVPEGYHPVGVPD
GYDSYYLNVMAGPKRVWKFHNDPDHEWILERD

Specific function: Involved in the isomerization of 5-deoxy-glucuronate (5DG) to 5-dehydro-2-deoxy-D-gluconate (DKG or 2-deoxy-5-keto-D- gluconate) [H]

COG id: COG3718

COG function: function code G; Uncharacterized enzyme involved in inositol metabolism

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isomerase iolB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR021120
- InterPro:   IPR014710 [H]

Pfam domain/function: PF04962 KduI [H]

EC number: NA

Molecular weight: Translated: 30884; Mature: 30753

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLLRKPLNERIAPGITFVQDINQANSPLSYVGFRLIEMEKGAIYQEELTDLECCIVAL
CCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCEEEEEECCCCEEHHHHCCCEEEEEEE
TGKITVSEGDNIFAEIGTRANVFEKIPTDSVFISGGRTFQVKADTEKARVALCYSQANRD
ECEEEECCCCCEEEECCCCHHHHHHCCCCEEEEECCEEEEEEECCCCCEEEEEEHHCCCC
LPTTLIKASDNSIEQRGKYQNKRLVHNILPDVSEVASSLLVVEVYTDGGNFSSYPPHKHD
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEEEEECCCCCCCCCCCCCC
RDNLPAESLLEESYYHEINPEQGFIFQRVYTDDRTLDETMAVEHQNAVIVPEGYHPVGVP
CCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHEECCCEEEEECCCCCCCCC
DGYDSYYLNVMAGPKRVWKFHNDPDHEWILERD
CCCCCEEEEEEECCHHHHCCCCCCCCCEEEECC
>Mature Secondary Structure 
GKLLRKPLNERIAPGITFVQDINQANSPLSYVGFRLIEMEKGAIYQEELTDLECCIVAL
CHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHCEEEEEECCCCEEHHHHCCCEEEEEEE
TGKITVSEGDNIFAEIGTRANVFEKIPTDSVFISGGRTFQVKADTEKARVALCYSQANRD
ECEEEECCCCCEEEECCCCHHHHHHCCCCEEEEECCEEEEEEECCCCCEEEEEEHHCCCC
LPTTLIKASDNSIEQRGKYQNKRLVHNILPDVSEVASSLLVVEVYTDGGNFSSYPPHKHD
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEEEEECCCCCCCCCCCCCC
RDNLPAESLLEESYYHEINPEQGFIFQRVYTDDRTLDETMAVEHQNAVIVPEGYHPVGVP
CCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHEECCCEEEEECCCCCCCCC
DGYDSYYLNVMAGPKRVWKFHNDPDHEWILERD
CCCCCEEEEEEECCHHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]