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The map label for this gene is tenA [H]

Identifier: 226222942

GI number: 226222942

Start: 338532

End: 339206

Strand: Direct

Name: tenA [H]

Synonym: Lm4b_00334

Alternate gene names: 226222942

Gene position: 338532-339206 (Clockwise)

Preceding gene: 226222941

Following gene: 226222943

Centisome position: 11.62

GC content: 46.52

Gene sequence:

>675_bases
GTGTTTGTTCACGGTTTTCAAGAAGAAGTAGGGGATTTATGGCAAGAAACGTTGCAGCATCCATTTGTGCAGAGTTTGGC
GGATGGGACCCTTGAGAAGGAGGCGTTTTACTATTATTTGCTCCAGGATGATTATTACTTGTCGCATTTTGAGAAGGTGA
TTGAGAAAAGTGTTGCGCAAGCTGGAACTGCGGAAATTGCTGCGGAAATGAGGGAAGTACAGGTGCGGCTTCAGCAGTCG
GAATTGTTGATGCGCGAACAGTTTTATCCGCGGGTTGGGCTAACGGAGCGCGATTTTTCCGTGCGAAAACCGGCACCAAC
TGCTTATCATTATACTTCTCACCTTTACCGGATGGCAGATTTTGGCAGTTTTGGGGTAACGATTGCGGCGCTTTTGCCGT
GTTATGCGCTTTATGCTGATATGGGGAAAATGTACGAAGGGGCGCGGAGTTCGGAGCCATTTTATCAAGAGTTGTTGGAT
AGTTATGTGGATGAAAATTATCAAAAAGTGGTGTTGCAGCAGAAGCGGTTGGTGGAACAGGCGGCGAGTATGGCGGATGC
GCGGGAGCTGGCCCTCATGAAGCAGGCTTTTCAAATTAGTGTGGAAATGGAATGGGCATTTTTTGATATGGCTTATAAAA
AACAAAATTGGCGTGGGAGTGTGAATTATGTTTGA

Upstream 100 bases:

>100_bases
GAATGTGAAAGTGACCGGAAAATTTATCGCGGCTACTGTTTCTTTGCTTATGCTAAGGGACAGTGGGCGCTTTTTTTATT
GTTCAAAAGGAGGAGTTTGA

Downstream 100 bases:

>100_bases
TTTTATGACGTTGGAAAAGGTGTGGGAAAAAGGGCCGTTGGTACATAATATTACGAATATCGTGGTCGCGAATGACTCGG
CGAACGGGTTGCTTGCGATT

Product: thiamin biosynthesis protein

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MFVHGFQEEVGDLWQETLQHPFVQSLADGTLEKEAFYYYLLQDDYYLSHFEKVIEKSVAQAGTAEIAAEMREVQVRLQQS
ELLMREQFYPRVGLTERDFSVRKPAPTAYHYTSHLYRMADFGSFGVTIAALLPCYALYADMGKMYEGARSSEPFYQELLD
SYVDENYQKVVLQQKRLVEQAASMADARELALMKQAFQISVEMEWAFFDMAYKKQNWRGSVNYV

Sequences:

>Translated_224_residues
MFVHGFQEEVGDLWQETLQHPFVQSLADGTLEKEAFYYYLLQDDYYLSHFEKVIEKSVAQAGTAEIAAEMREVQVRLQQS
ELLMREQFYPRVGLTERDFSVRKPAPTAYHYTSHLYRMADFGSFGVTIAALLPCYALYADMGKMYEGARSSEPFYQELLD
SYVDENYQKVVLQQKRLVEQAASMADARELALMKQAFQISVEMEWAFFDMAYKKQNWRGSVNYV
>Mature_224_residues
MFVHGFQEEVGDLWQETLQHPFVQSLADGTLEKEAFYYYLLQDDYYLSHFEKVIEKSVAQAGTAEIAAEMREVQVRLQQS
ELLMREQFYPRVGLTERDFSVRKPAPTAYHYTSHLYRMADFGSFGVTIAALLPCYALYADMGKMYEGARSSEPFYQELLD
SYVDENYQKVVLQQKRLVEQAASMADARELALMKQAFQISVEMEWAFFDMAYKKQNWRGSVNYV

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 26133; Mature: 26133

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVHGFQEEVGDLWQETLQHPFVQSLADGTLEKEAFYYYLLQDDYYLSHFEKVIEKSVAQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEECCCHHHHHHHHHHHHHHHH
AGTAEIAAEMREVQVRLQQSELLMREQFYPRVGLTERDFSVRKPAPTAYHYTSHLYRMAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FGSFGVTIAALLPCYALYADMGKMYEGARSSEPFYQELLDSYVDENYQKVVLQQKRLVEQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AASMADARELALMKQAFQISVEMEWAFFDMAYKKQNWRGSVNYV
HHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFVHGFQEEVGDLWQETLQHPFVQSLADGTLEKEAFYYYLLQDDYYLSHFEKVIEKSVAQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEECCCHHHHHHHHHHHHHHHH
AGTAEIAAEMREVQVRLQQSELLMREQFYPRVGLTERDFSVRKPAPTAYHYTSHLYRMAD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FGSFGVTIAALLPCYALYADMGKMYEGARSSEPFYQELLDSYVDENYQKVVLQQKRLVEQ
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AASMADARELALMKQAFQISVEMEWAFFDMAYKKQNWRGSVNYV
HHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]