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The map label for this gene is thiM [H]

Identifier: 226222943

GI number: 226222943

Start: 339199

End: 340008

Strand: Direct

Name: thiM [H]

Synonym: Lm4b_00335

Alternate gene names: 226222943

Gene position: 339199-340008 (Clockwise)

Preceding gene: 226222942

Following gene: 226222944

Centisome position: 11.65

GC content: 48.4

Gene sequence:

>810_bases
ATGTTTGATTTTATGACGTTGGAAAAGGTGTGGGAAAAAGGGCCGTTGGTACATAATATTACGAATATCGTGGTCGCGAA
TGACTCGGCGAACGGGTTGCTTGCGATTGGTGCGTCACCGATTATGGCTTCTGCGAAAGAGGAAATGGATGAACTTGCGA
AAATGGCGGATGTGCTCGTGATTAATATTGGGACGCTAGACGGGGAGCTTGTTACAGCGATGAAAATTGCTGGACGTGCG
GCAAATGTTGCTGGAACGCCGGTTGTGCTTGACCCGGTCGGTGTTGGTGCGACTTCATACCGTCGTAAAGTGGTGCAAGA
ATTATTGGCTGAAATCCAGTTTACGGCGATTCGTGGAAATGCGGGAGAGCTTGCGGCGATTGCTGGTGAAGCTTGGGAAG
CGAAAGGCGTGGATGCGGGTGTTGGTTCGGCGGATGTGCTGAGCATTGCTGAAAAAGTGGCGAATGAATGGAGCACGGTT
GTTATTATTAGCGGCGAAGTAGATGTGATTTCAGACGGAACGCGTTTTGCAAAAGTGGCTAATGGTAGCGCACTGCTTCC
AAGAATTACTGGTTCTGGTTGTTTGCTCAGTGCAGTTTGCGGTAGTTTTATGGCCGTTCAGGATGATGCTTTTCGAGCTA
GTGTCGAAGCATGCGCGAGTTATGCGGTGGCTTCTGAGTACGCGGAAATCGAATTAGAAAGAAAACTTCCAGGTTCATTT
CGACCACAATTTTTAGATGCTCTTGCTAGTTGGTCGGTCGAAAAAACGCGGGCCAAAGCCAAAATCCAAGAAAGTGGTGA
ACACAAATGA

Upstream 100 bases:

>100_bases
CTGGCCCTCATGAAGCAGGCTTTTCAAATTAGTGTGGAAATGGAATGGGCATTTTTTGATATGGCTTATAAAAAACAAAA
TTGGCGTGGGAGTGTGAATT

Downstream 100 bases:

>100_bases
TTTTCAAACAAGTATTAACGATAGCTGGTTCAGATTCTGGCGGCGGCGCGGGGATACAAGCGGATATCAAGACATTTCAA
GAGCGTAAAACCTTTGGCAT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MFDFMTLEKVWEKGPLVHNITNIVVANDSANGLLAIGASPIMASAKEEMDELAKMADVLVINIGTLDGELVTAMKIAGRA
ANVAGTPVVLDPVGVGATSYRRKVVQELLAEIQFTAIRGNAGELAAIAGEAWEAKGVDAGVGSADVLSIAEKVANEWSTV
VIISGEVDVISDGTRFAKVANGSALLPRITGSGCLLSAVCGSFMAVQDDAFRASVEACASYAVASEYAEIELERKLPGSF
RPQFLDALASWSVEKTRAKAKIQESGEHK

Sequences:

>Translated_269_residues
MFDFMTLEKVWEKGPLVHNITNIVVANDSANGLLAIGASPIMASAKEEMDELAKMADVLVINIGTLDGELVTAMKIAGRA
ANVAGTPVVLDPVGVGATSYRRKVVQELLAEIQFTAIRGNAGELAAIAGEAWEAKGVDAGVGSADVLSIAEKVANEWSTV
VIISGEVDVISDGTRFAKVANGSALLPRITGSGCLLSAVCGSFMAVQDDAFRASVEACASYAVASEYAEIELERKLPGSF
RPQFLDALASWSVEKTRAKAKIQESGEHK
>Mature_269_residues
MFDFMTLEKVWEKGPLVHNITNIVVANDSANGLLAIGASPIMASAKEEMDELAKMADVLVINIGTLDGELVTAMKIAGRA
ANVAGTPVVLDPVGVGATSYRRKVVQELLAEIQFTAIRGNAGELAAIAGEAWEAKGVDAGVGSADVLSIAEKVANEWSTV
VIISGEVDVISDGTRFAKVANGSALLPRITGSGCLLSAVCGSFMAVQDDAFRASVEACASYAVASEYAEIELERKLPGSF
RPQFLDALASWSVEKTRAKAKIQESGEHK

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=249, Percent_Identity=40.5622489959839, Blast_Score=164, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6325042, Length=271, Percent_Identity=31.7343173431734, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 28199; Mature: 28199

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDFMTLEKVWEKGPLVHNITNIVVANDSANGLLAIGASPIMASAKEEMDELAKMADVLV
CCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEE
INIGTLDGELVTAMKIAGRAANVAGTPVVLDPVGVGATSYRRKVVQELLAEIQFTAIRGN
EEECCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECC
AGELAAIAGEAWEAKGVDAGVGSADVLSIAEKVANEWSTVVIISGEVDVISDGTRFAKVA
CCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCEEEEC
NGSALLPRITGSGCLLSAVCGSFMAVQDDAFRASVEACASYAVASEYAEIELERKLPGSF
CCCEEECEECCCCHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
RPQFLDALASWSVEKTRAKAKIQESGEHK
CHHHHHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFDFMTLEKVWEKGPLVHNITNIVVANDSANGLLAIGASPIMASAKEEMDELAKMADVLV
CCCHHHHHHHHHCCCCEEEEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEE
INIGTLDGELVTAMKIAGRAANVAGTPVVLDPVGVGATSYRRKVVQELLAEIQFTAIRGN
EEECCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECC
AGELAAIAGEAWEAKGVDAGVGSADVLSIAEKVANEWSTVVIISGEVDVISDGTRFAKVA
CCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCEEEEC
NGSALLPRITGSGCLLSAVCGSFMAVQDDAFRASVEACASYAVASEYAEIELERKLPGSF
CCCEEECEECCCCHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
RPQFLDALASWSVEKTRAKAKIQESGEHK
CHHHHHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]