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The map label for this gene is rlmH [H]

Identifier: 226222929

GI number: 226222929

Start: 326811

End: 327290

Strand: Direct

Name: rlmH [H]

Synonym: Lm4b_00321

Alternate gene names: 226222929

Gene position: 326811-327290 (Clockwise)

Preceding gene: 226222928

Following gene: 226222931

Centisome position: 11.22

GC content: 46.46

Gene sequence:

>480_bases
ATGAATATTCAAATTGTAACGGTCGGGAAATTAAAAGAAAAATATTTAGTGCAAGGAATCGCCGAATATTTAAAACGACT
TAGCGCCTATGCGAAAGTGACGATCGTTGAAGTTCCGGATGAGAAAGCTCCGGAAGTGCTGAGTGATGCCGAAATGAAGC
AAGTAAAAGATAAAGAGGGTGCGCGGATTTTAGCAAAGATTCCGGACGATGCCCATGTGATTGCGCTGGCGATTGATGGG
AAAATGAAGTCGAGTGAAGAATTTGCGGCGGATTTGGATAAGCTGGCGACTTATGGTAAGAGTAAGGTGACGTTTGTGAT
TGGTGGATCGCTGGGGCTTAGTGAGGCGGTGTTGAAGCGGAGTAATGAGCGGATTTCGTTTGGGAGGTTAACGCTGCCAC
ACCAGTTGATGAGATTGGTGCTGGTGGAGCAGGTTTACCGGGCGTTTCGAATTGTTCGGGGGGAGCCTTATCATAAATAG

Upstream 100 bases:

>100_bases
ATAACTTTTTATGGAAAGCTGCTCTTATTTTATTTTGACTAGTGCAATGAGGGATTTTTGATGTATGATAAAAGTATTAA
AAAAGATAAGGGTGGCATAA

Downstream 100 bases:

>100_bases
CTAGCTGGTTGAAAGGGTTGTAATCAACAACTTAACTTTTGAATGCTGGTTTACTTTTTTCGATAAGCCATTTTTCCCCA
TTTGAAATTGTATGATCTGC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNIQIVTVGKLKEKYLVQGIAEYLKRLSAYAKVTIVEVPDEKAPEVLSDAEMKQVKDKEGARILAKIPDDAHVIALAIDG
KMKSSEEFAADLDKLATYGKSKVTFVIGGSLGLSEAVLKRSNERISFGRLTLPHQLMRLVLVEQVYRAFRIVRGEPYHK

Sequences:

>Translated_159_residues
MNIQIVTVGKLKEKYLVQGIAEYLKRLSAYAKVTIVEVPDEKAPEVLSDAEMKQVKDKEGARILAKIPDDAHVIALAIDG
KMKSSEEFAADLDKLATYGKSKVTFVIGGSLGLSEAVLKRSNERISFGRLTLPHQLMRLVLVEQVYRAFRIVRGEPYHK
>Mature_159_residues
MNIQIVTVGKLKEKYLVQGIAEYLKRLSAYAKVTIVEVPDEKAPEVLSDAEMKQVKDKEGARILAKIPDDAHVIALAIDG
KMKSSEEFAADLDKLATYGKSKVTFVIGGSLGLSEAVLKRSNERISFGRLTLPHQLMRLVLVEQVYRAFRIVRGEPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016051
- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 17741; Mature: 17741

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQIVTVGKLKEKYLVQGIAEYLKRLSAYAKVTIVEVPDEKAPEVLSDAEMKQVKDKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCCC
ARILAKIPDDAHVIALAIDGKMKSSEEFAADLDKLATYGKSKVTFVIGGSLGLSEAVLKR
CEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHC
SNERISFGRLTLPHQLMRLVLVEQVYRAFRIVRGEPYHK
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNIQIVTVGKLKEKYLVQGIAEYLKRLSAYAKVTIVEVPDEKAPEVLSDAEMKQVKDKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCCC
ARILAKIPDDAHVIALAIDGKMKSSEEFAADLDKLATYGKSKVTFVIGGSLGLSEAVLKR
CEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHC
SNERISFGRLTLPHQLMRLVLVEQVYRAFRIVRGEPYHK
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]