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The map label for this gene is ada [H]

Identifier: 226222717

GI number: 226222717

Start: 83695

End: 84747

Strand: Direct

Name: ada [H]

Synonym: Lm4b_00082

Alternate gene names: 226222717

Gene position: 83695-84747 (Clockwise)

Preceding gene: 226222716

Following gene: 226222719

Centisome position: 2.87

GC content: 41.6

Gene sequence:

>1053_bases
GTGATAACGAATCAGCGGGATATTGATAAATATTATGATATGTTAGTGGAGAAAAATTCGAATTATGAAGGCGTATTTTT
TGTTGGAGTGAAAACGACGGGAATACTTTGTCGCCCAACGTGTCCGGCGAAGAAACCTTTAAAAGAGAATTGTGAGTTTT
TCAGTACAGCCAAGGAGGCGCTACTTGCATCTTACCGGCCTTGCAAGCGTTGTGAACCACTCTCCAATCCGACGAAATTG
TCGCCAGCTGTGAAGTTGCTTGTTGATGCAATTGAGAAAAATCCAGAGAAAAAATGGACGGATAAAGATTTTGATGAATT
ATCCATTAGCGCAAATACAGCGCGGCGCCAGTTTAAGAAGCAGTTTGGGATGACTTTTATTGAGTATGCGCGTTCGCGGC
GTCTCGGTCTTGCTTTTAAGCATATTCGGGGTGGAAATTCGATCATTAATGCACAAATTGAAAGTGGTTATGAGTCTGGA
AATGGGTTCCGAGATGCTTTTTCAAAAACGATGGGGGAGGTTCCTCACAAATCAAACGATATCATCATTTTATACTCCGC
TTGGCTTGAGACTAAACTTGGATCAATGCTCGCAATTTCTGATGATGAAAGTCTATTGCTTCTGGAATTTGTTGATCGAA
AAGGCTTGGAAACAGAAATTAAAAAATTACGAATGCGCTTAAACGCGGCTATTACACCTGAAAAAACAGTAATAATTCAA
CAAATAGAAGCCGAATTAGCGCGATATTTTAATGGTGAGTTGATTGATTTTAAAACACCGATTTGCTATATCGGGTCAGA
TTTTCAACAGAGTGTCTGGAATGAACTGCGACGAATTCCGATAGGCGAAACAATTTCGTACAAAAGTTTGGCAGAAAAAC
TAGGGCGGCCAACAGCAAGTCGAGCTGTGGCAAGAGCAAATGGGGCTAACCAATTATCCCTCATCGTCCCGTGCCATCGT
GTAATCAATACTAACGGAGCATTAGGAGGCTACGGTGGCGGTCTTGCGCGCAAAGAATGGCTAATTAAACATGAAAAGAT
GGTTCCAAGGTAA

Upstream 100 bases:

>100_bases
TCGGTGTGAAAAGAATCAGTTATGGACCGTTTAGTTTTTTTCAGGCTAATTTAGCGATTCAGCTGGAAGTTGAGCGGATT
TTCGAAGGAGGGAAAGCCTT

Downstream 100 bases:

>100_bases
CGCGGAACCATCTTTTTTCAATTATTTAACCCGTATTCCAAACAACGGCATCAAAGCAGGAGCATGCGAAAAATCAATGC
TCACGCCCTTCAAATCTTGC

Product: Ada protein (O6-methylguanine-DNA methyltransferase)

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MITNQRDIDKYYDMLVEKNSNYEGVFFVGVKTTGILCRPTCPAKKPLKENCEFFSTAKEALLASYRPCKRCEPLSNPTKL
SPAVKLLVDAIEKNPEKKWTDKDFDELSISANTARRQFKKQFGMTFIEYARSRRLGLAFKHIRGGNSIINAQIESGYESG
NGFRDAFSKTMGEVPHKSNDIIILYSAWLETKLGSMLAISDDESLLLLEFVDRKGLETEIKKLRMRLNAAITPEKTVIIQ
QIEAELARYFNGELIDFKTPICYIGSDFQQSVWNELRRIPIGETISYKSLAEKLGRPTASRAVARANGANQLSLIVPCHR
VINTNGALGGYGGGLARKEWLIKHEKMVPR

Sequences:

>Translated_350_residues
MITNQRDIDKYYDMLVEKNSNYEGVFFVGVKTTGILCRPTCPAKKPLKENCEFFSTAKEALLASYRPCKRCEPLSNPTKL
SPAVKLLVDAIEKNPEKKWTDKDFDELSISANTARRQFKKQFGMTFIEYARSRRLGLAFKHIRGGNSIINAQIESGYESG
NGFRDAFSKTMGEVPHKSNDIIILYSAWLETKLGSMLAISDDESLLLLEFVDRKGLETEIKKLRMRLNAAITPEKTVIIQ
QIEAELARYFNGELIDFKTPICYIGSDFQQSVWNELRRIPIGETISYKSLAEKLGRPTASRAVARANGANQLSLIVPCHR
VINTNGALGGYGGGLARKEWLIKHEKMVPR
>Mature_350_residues
MITNQRDIDKYYDMLVEKNSNYEGVFFVGVKTTGILCRPTCPAKKPLKENCEFFSTAKEALLASYRPCKRCEPLSNPTKL
SPAVKLLVDAIEKNPEKKWTDKDFDELSISANTARRQFKKQFGMTFIEYARSRRLGLAFKHIRGGNSIINAQIESGYESG
NGFRDAFSKTMGEVPHKSNDIIILYSAWLETKLGSMLAISDDESLLLLEFVDRKGLETEIKKLRMRLNAAITPEKTVIIQ
QIEAELARYFNGELIDFKTPICYIGSDFQQSVWNELRRIPIGETISYKSLAEKLGRPTASRAVARANGANQLSLIVPCHR
VINTNGALGGYGGGLARKEWLIKHEKMVPR

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=114, Percent_Identity=40.3508771929825, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788542, Length=350, Percent_Identity=32, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=52.9411764705882, Blast_Score=116, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI115533068, Length=78, Percent_Identity=47.4358974358974, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=78, Percent_Identity=47.4358974358974, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=45.7831325301205, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137554, Length=87, Percent_Identity=50.5747126436782, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39484; Mature: 39484

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITNQRDIDKYYDMLVEKNSNYEGVFFVGVKTTGILCRPTCPAKKPLKENCEFFSTAKEA
CCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHH
LLASYRPCKRCEPLSNPTKLSPAVKLLVDAIEKNPEKKWTDKDFDELSISANTARRQFKK
HHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECHHHHHHHHHH
QFGMTFIEYARSRRLGLAFKHIRGGNSIINAQIESGYESGNGFRDAFSKTMGEVPHKSND
HHCCHHHHHHHHCCHHHHHHHHCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHCCCCCCCC
IIILYSAWLETKLGSMLAISDDESLLLLEFVDRKGLETEIKKLRMRLNAAITPEKTVIIQ
EEEEEHHHHHHHCCCEEEECCCCCEEEEEHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
QIEAELARYFNGELIDFKTPICYIGSDFQQSVWNELRRIPIGETISYKSLAEKLGRPTAS
HHHHHHHHHHCCCEEEECCCEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHH
RAVARANGANQLSLIVPCHRVINTNGALGGYGGGLARKEWLIKHEKMVPR
HHHHHCCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MITNQRDIDKYYDMLVEKNSNYEGVFFVGVKTTGILCRPTCPAKKPLKENCEFFSTAKEA
CCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHH
LLASYRPCKRCEPLSNPTKLSPAVKLLVDAIEKNPEKKWTDKDFDELSISANTARRQFKK
HHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECHHHHHHHHHH
QFGMTFIEYARSRRLGLAFKHIRGGNSIINAQIESGYESGNGFRDAFSKTMGEVPHKSND
HHCCHHHHHHHHCCHHHHHHHHCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHCCCCCCCC
IIILYSAWLETKLGSMLAISDDESLLLLEFVDRKGLETEIKKLRMRLNAAITPEKTVIIQ
EEEEEHHHHHHHCCCEEEECCCCCEEEEEHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHH
QIEAELARYFNGELIDFKTPICYIGSDFQQSVWNELRRIPIGETISYKSLAEKLGRPTAS
HHHHHHHHHHCCCEEEECCCEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHH
RAVARANGANQLSLIVPCHRVINTNGALGGYGGGLARKEWLIKHEKMVPR
HHHHHCCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]