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The map label for this gene is yiaE [C]

Identifier: 226222719

GI number: 226222719

Start: 85538

End: 86494

Strand: Direct

Name: yiaE [C]

Synonym: Lm4b_00084

Alternate gene names: 226222719

Gene position: 85538-86494 (Clockwise)

Preceding gene: 226222717

Following gene: 226222720

Centisome position: 2.94

GC content: 43.99

Gene sequence:

>957_bases
ATGACAGCGAGCGTATTTGTAGCAGGAAAATTACTACCAGAAACGATGGAAGCTCTTAGCAAATGGGACGTTAAAGCTTT
TTCTGGTGAGGAAAATATTACAGAAGCAGAGTTAATAAAAAATGTAACGGAAGTGGATGCGATTATTTGCCCGCTTTCTT
CCCCAATTTCGGCAAAAGTATTGGAATCGGCAAAAAATCTTAAAATCGTGGCGAATATTGGTGCCGGTTTTGACAATATC
GATGTAAAAAAAGCGCAAGAATTGGGCATTGCAGTGACCAATACGCCAGATGTGTCAACGGAAGCAACGGCGGAACTAAC
GCTTGGATTGATTTTGGACGTAGCACGGAGAATTTCAGAAGGCGACCGATTATGCCGCGAAACACCCGAGCAATTCAAAG
GCTGGGCACCAACGTTCTTTTTAGGTACTGAATTAAGTGGCAAAACGCTCGGAATTATTGGATTAGGAAGAATCGGACAA
GCTGTTGCAAAACGTGCGGCTGCTTTTGGAATGAAAATTATTTATTCTGGGCACCATCCGAAAGAAGCAGCGAAAGAGTG
GAATGCAGAGTTTGTGAGCCAGGAAGAATTATTGAAGCGCAGCGATGTTGTAACAATCCATGCGGCATACAGTCCCAGCT
TGAAGTATCTGTTGAACGAAACAACACTGAAAACGATGAAATCAAGTGCCTTTTTGATCAATGCTGCTCGCGGTCCTGTT
GTAGAGGAAGCTGCACTGATTAAGGCATTAGAAGCAGGCGTTATCGCCGGCGCTGCGCTGGATGTGTTTGAGTTCGAGCC
TAAGATTGGCGAGGATTTGGCTAAACTGGATAATGTCGTATTAACACCCCATATCGGAAACGCAACTGTGGAAACACGCA
CAGCAATGGGCAAAATAGCCATTGCAAATGTTGAAGCTGTCTTAGCAGGAAAAGCACCACTACATTCTGTTTATTAA

Upstream 100 bases:

>100_bases
CCGTATTTTGCTAGGAAAAGGAGCGGAAAATCGTTTACCTTTCATAAAAAACGGATTACAATGAATGTAGAATAAAAATG
GCAGTTGAGGAGTGAACGTG

Downstream 100 bases:

>100_bases
TACCGAAAAATATAGCCGCAGCGAGTGGCTGCGGCTGTGTTTTATCGCTGAATTATGGTATAATATTTTTTGTCGGAATA
CGACAACGGGTTGTTAGCTC

Product: phosphoglycerate dehydrogenase

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTASVFVAGKLLPETMEALSKWDVKAFSGEENITEAELIKNVTEVDAIICPLSSPISAKVLESAKNLKIVANIGAGFDNI
DVKKAQELGIAVTNTPDVSTEATAELTLGLILDVARRISEGDRLCRETPEQFKGWAPTFFLGTELSGKTLGIIGLGRIGQ
AVAKRAAAFGMKIIYSGHHPKEAAKEWNAEFVSQEELLKRSDVVTIHAAYSPSLKYLLNETTLKTMKSSAFLINAARGPV
VEEAALIKALEAGVIAGAALDVFEFEPKIGEDLAKLDNVVLTPHIGNATVETRTAMGKIAIANVEAVLAGKAPLHSVY

Sequences:

>Translated_318_residues
MTASVFVAGKLLPETMEALSKWDVKAFSGEENITEAELIKNVTEVDAIICPLSSPISAKVLESAKNLKIVANIGAGFDNI
DVKKAQELGIAVTNTPDVSTEATAELTLGLILDVARRISEGDRLCRETPEQFKGWAPTFFLGTELSGKTLGIIGLGRIGQ
AVAKRAAAFGMKIIYSGHHPKEAAKEWNAEFVSQEELLKRSDVVTIHAAYSPSLKYLLNETTLKTMKSSAFLINAARGPV
VEEAALIKALEAGVIAGAALDVFEFEPKIGEDLAKLDNVVLTPHIGNATVETRTAMGKIAIANVEAVLAGKAPLHSVY
>Mature_317_residues
TASVFVAGKLLPETMEALSKWDVKAFSGEENITEAELIKNVTEVDAIICPLSSPISAKVLESAKNLKIVANIGAGFDNID
VKKAQELGIAVTNTPDVSTEATAELTLGLILDVARRISEGDRLCRETPEQFKGWAPTFFLGTELSGKTLGIIGLGRIGQA
VAKRAAAFGMKIIYSGHHPKEAAKEWNAEFVSQEELLKRSDVVTIHAAYSPSLKYLLNETTLKTMKSSAFLINAARGPVV
EEAALIKALEAGVIAGAALDVFEFEPKIGEDLAKLDNVVLTPHIGNATVETRTAMGKIAIANVEAVLAGKAPLHSVY

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=319, Percent_Identity=36.9905956112853, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI23308577, Length=282, Percent_Identity=37.5886524822695, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI61743967, Length=264, Percent_Identity=40.1515151515151, Blast_Score=165, Evalue=5e-41,
Organism=Homo sapiens, GI4557497, Length=264, Percent_Identity=39.7727272727273, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI145580578, Length=264, Percent_Identity=37.5, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4557499, Length=264, Percent_Identity=37.5, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI145580575, Length=264, Percent_Identity=37.5, Blast_Score=156, Evalue=3e-38,
Organism=Escherichia coli, GI87082289, Length=260, Percent_Identity=40.7692307692308, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1787645, Length=288, Percent_Identity=30.5555555555556, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1789279, Length=249, Percent_Identity=34.136546184739, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788660, Length=261, Percent_Identity=27.9693486590038, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081824, Length=240, Percent_Identity=27.5, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=264, Percent_Identity=38.6363636363636, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI25147481, Length=264, Percent_Identity=34.469696969697, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324055, Length=244, Percent_Identity=38.5245901639344, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322116, Length=283, Percent_Identity=36.3957597173145, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6320925, Length=283, Percent_Identity=36.0424028268551, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6324964, Length=250, Percent_Identity=28.4, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321253, Length=281, Percent_Identity=26.6903914590747, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6325144, Length=169, Percent_Identity=26.6272189349112, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324980, Length=162, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571528, Length=283, Percent_Identity=42.756183745583, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI28574284, Length=291, Percent_Identity=36.0824742268041, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45551003, Length=291, Percent_Identity=36.0824742268041, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24585514, Length=291, Percent_Identity=36.0824742268041, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI28574282, Length=291, Percent_Identity=36.0824742268041, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45552429, Length=291, Percent_Identity=36.0824742268041, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24646446, Length=262, Percent_Identity=38.9312977099237, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646448, Length=262, Percent_Identity=38.9312977099237, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646452, Length=262, Percent_Identity=38.9312977099237, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646450, Length=262, Percent_Identity=38.9312977099237, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI62472511, Length=262, Percent_Identity=38.9312977099237, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI19921140, Length=289, Percent_Identity=35.2941176470588, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI28574286, Length=312, Percent_Identity=33.6538461538462, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24585516, Length=285, Percent_Identity=32.280701754386, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 33881; Mature: 33750

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASVFVAGKLLPETMEALSKWDVKAFSGEENITEAELIKNVTEVDAIICPLSSPISAKV
CCCEEEEEHHHCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHEEECCCCCHHHHH
LESAKNLKIVANIGAGFDNIDVKKAQELGIAVTNTPDVSTEATAELTLGLILDVARRISE
HHCCCCEEEEEECCCCCCCCCHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
GDRLCRETPEQFKGWAPTFFLGTELSGKTLGIIGLGRIGQAVAKRAAAFGMKIIYSGHHP
HHHHHHCCHHHHCCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCEEEEECCCCC
KEAAKEWNAEFVSQEELLKRSDVVTIHAAYSPSLKYLLNETTLKTMKSSAFLINAARGPV
HHHHHHHCHHHCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCH
VEEAALIKALEAGVIAGAALDVFEFEPKIGEDLAKLDNVVLTPHIGNATVETRTAMGKIA
HHHHHHHHHHHHHHHHCCEEHHEECCCCHHHHHHHHCCEEEECCCCCCCCHHHHHHCEEE
IANVEAVLAGKAPLHSVY
EEEHEEEEECCCCCCCCC
>Mature Secondary Structure 
TASVFVAGKLLPETMEALSKWDVKAFSGEENITEAELIKNVTEVDAIICPLSSPISAKV
CCEEEEEHHHCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHEEECCCCCHHHHH
LESAKNLKIVANIGAGFDNIDVKKAQELGIAVTNTPDVSTEATAELTLGLILDVARRISE
HHCCCCEEEEEECCCCCCCCCHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
GDRLCRETPEQFKGWAPTFFLGTELSGKTLGIIGLGRIGQAVAKRAAAFGMKIIYSGHHP
HHHHHHCCHHHHCCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCEEEEECCCCC
KEAAKEWNAEFVSQEELLKRSDVVTIHAAYSPSLKYLLNETTLKTMKSSAFLINAARGPV
HHHHHHHCHHHCCHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCH
VEEAALIKALEAGVIAGAALDVFEFEPKIGEDLAKLDNVVLTPHIGNATVETRTAMGKIA
HHHHHHHHHHHHHHHHCCEEHHEECCCCHHHHHHHHCCEEEECCCCCCCCHHHHHHCEEE
IANVEAVLAGKAPLHSVY
EEEHEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA