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The map label for this gene is manX [C]

Identifier: 226222661

GI number: 226222661

Start: 27030

End: 27431

Strand: Direct

Name: manX [C]

Synonym: Lm4b_00024

Alternate gene names: 226222661

Gene position: 27030-27431 (Clockwise)

Preceding gene: 226222660

Following gene: 226222662

Centisome position: 0.93

GC content: 41.29

Gene sequence:

>402_bases
TTGAATTTTATTATCGCAGCACATGGCCGGTACGCACAGGAAGTGAAAAATAGCTGTCAGATGATTACTGGCCAAACAAC
TAATATCTCAGCAATTACTTTTACCGAAGAAATGGGGGTGACGGATGTATTAGACGCCTACACGGCCGTTTATTCACCCG
GAAATGAAACCGCTATCATAGTTGATATTGTTGGTGGAACTCCGTGTAATGCGGCTCAAATGTTTAGTACAAAACATCCA
GAAGTTAAAGTTGTATCAGGTTTGTCGCTAGGATTACTCATTCCACTTAGCCTTGGAGAAAGTTTAGAGGAGGCGATGCT
TGGAGCAAAAGAAAATATTCAATTTGTAGAGCAAAAAGCGAGTTATACGATAGTAAGTGATGATGGGGAGGAAGAGGACT
GA

Upstream 100 bases:

>100_bases
GCCAGAGGGGCAACCCTATGAATATATTGAAAGCTACTTGCGGCATAATTTTTACTCTATTGAAATTGAAAAGAAAAAAC
AATAGAAGGGAGTTAATTAC

Downstream 100 bases:

>100_bases
TGGGAAGCAACGGAATTAAACACGTACGCGTAGATGAGAGACTAATTCACGGGCAAGTAGCAACAATGTGGACCAATACT
ATAAAAGCAACGCGAATCAT

Product: PTS system, fructose-specific IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: PTS System Fructose Family IIA Component; PTS System Transporter Subunit IIAB

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MNFIIAAHGRYAQEVKNSCQMITGQTTNISAITFTEEMGVTDVLDAYTAVYSPGNETAIIVDIVGGTPCNAAQMFSTKHP
EVKVVSGLSLGLLIPLSLGESLEEAMLGAKENIQFVEQKASYTIVSDDGEEED

Sequences:

>Translated_133_residues
MNFIIAAHGRYAQEVKNSCQMITGQTTNISAITFTEEMGVTDVLDAYTAVYSPGNETAIIVDIVGGTPCNAAQMFSTKHP
EVKVVSGLSLGLLIPLSLGESLEEAMLGAKENIQFVEQKASYTIVSDDGEEED
>Mature_133_residues
MNFIIAAHGRYAQEVKNSCQMITGQTTNISAITFTEEMGVTDVLDAYTAVYSPGNETAIIVDIVGGTPCNAAQMFSTKHP
EVKVVSGLSLGLLIPLSLGESLEEAMLGAKENIQFVEQKASYTIVSDDGEEED

Specific function: This Is A Component Of The Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System (Pts), A Major Carbohydrate Active -Transport System. The IIcd Domains Contain The Sugar Binding Site And The Transmembrane Channel; The IIa Domain Contains The Prim

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69

Molecular weight: Translated: 14221; Mature: 14221

Theoretical pI: Translated: 3.93; Mature: 3.93

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFIIAAHGRYAQEVKNSCQMITGQTTNISAITFTEEMGVTDVLDAYTAVYSPGNETAII
CCEEEECCCCHHHHHHHHHEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEE
VDIVGGTPCNAAQMFSTKHPEVKVVSGLSLGLLIPLSLGESLEEAMLGAKENIQFVEQKA
EEECCCCCCCHHHHHCCCCCCEEEECCCCEEEEEEECCCCHHHHHHCCCCCCHHHHCCCC
SYTIVSDDGEEED
CEEEECCCCCCCC
>Mature Secondary Structure
MNFIIAAHGRYAQEVKNSCQMITGQTTNISAITFTEEMGVTDVLDAYTAVYSPGNETAII
CCEEEECCCCHHHHHHHHHEEECCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEE
VDIVGGTPCNAAQMFSTKHPEVKVVSGLSLGLLIPLSLGESLEEAMLGAKENIQFVEQKA
EEECCCCCCCHHHHHCCCCCCEEEECCCCEEEEEEECCCCHHHHHHCCCCCCHHHHCCCC
SYTIVSDDGEEED
CEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA