Click here to switch to the map view.

The map label for this gene is dnaA

Identifier: 226222640

GI number: 226222640

Start: 319

End: 1674

Strand: Direct

Name: dnaA

Synonym: Lm4b_00001

Alternate gene names: 226222640

Gene position: 319-1674 (Clockwise)

Preceding gene: NA

Following gene: 226222641

Centisome position: 0.01

GC content: 37.54

Gene sequence:

>1356_bases
GTGCAATCAATTGAAGACATCTGGCAGGAAACACTGCAAATCGTAAAAAAAAATATGAGTAAACCTAGTTACGACACATG
GATGAAATCAACAACCGCTCATTCACTTGAAGGTAACACGTTTATTATTTCAGCGCCCAATAATTTTGTTCGCGATTGGT
TAGAGAAGAGTTACACGCAATTTATCGCTAACATTTTGCAAGAAATAACTGGTCGCTTATTTGATGTCCGCTTTATTGAT
GGCGAGCAGGAAGAAAACTTTGAATACACTGTGATTAAACCAAATCCAGCGTTAGATGAAGATGGCGTTGAAATTGGAAA
ACATATGCTTAATCCGCGTTATGTTTTTGATACTTTTGTCATTGGTTCAGGGAACAGATTTGCCCACGCAGCATCACTTG
CAGTAGCCGAAGCACCAGCGAAAGCATATAATCCACTCTTCATTTATGGAGGAGTTGGCCTCGGTAAAACACATTTAATG
CACGCAGTTGGCCACTATGTTCAACAACATAAAGATAATGCGAAAGTAATGTACCTTTCCAGCGAAAAATTCACCAATGA
GTTTATTAGCTCTATTCGTGATAATAAAACCGAAGAATTCCGTACAAAATACCGGAATGTGGATGTCTTACTTATTGATG
ATATTCAATTTTTAGCAGGTAAAGAAGGAACACAAGAGGAATTTTTCCATACATTTAACACACTTTATGATGAACAAAAG
CAAATTATTATTTCCAGTGACCGACCACCGAAAGAAATTCCAACATTGGAAGATCGACTGAGATCCCGCTTTGAATGGGG
CCTAATTACTGATATTACGCCACCAGACTTAGAAACACGGATCGCCATTTTACGTAAAAAAGCAAAAGCAGACGGATTAG
ATATTCCAAATGAAGTCATGCTTTACATCGCAAACCAAATTGATTCGAATATTCGCGAGCTAGAAGGCGCACTCATCCGA
GTAGTTGCTTATTCTTCCCTCGTTAATAAAGATATAACAGCTGGTCTTGCAGCAGAAGCACTAAAAGATATTATCCCCTC
TTCTAAATCACAAGTTATTACAATTAGTGGTATTCAAGAAGCAGTCGGTGAATATTTCCATGTTCGTTTAGAAGATTTTA
AAGCAAAAAAACGGACGAAAAGTATCGCATTCCCGCGCCAAATCGCTATGTATCTTTCAAGAGAGCTTACAGATGCCTCA
TTACCAAAAATCGGTGATGAATTTGGTGGTCGAGATCATACAACTGTCATCCATGCACATGAAAAAATATCGCAACTACT
AAAAACCGATCAAGTGTTGAAAAATGACCTTGCCGAAATTGAAAAAAATTTAAGAAAAGCACAAAATATGTTTTAA

Upstream 100 bases:

>100_bases
AATTTTTTAACAGTGTTTGGATAACCTTATCCATGGCTTTTTCTATCTGTGGATAACTTTATAGCATCCATTTACATTAC
ATAAAAAAGGGGGGGTACTA

Downstream 100 bases:

>100_bases
TAGACCTGTGTACAATGTGGATAACTGAAACATACTTACCCACAAGTTATCAACATGTGGAAAACTTTATGTCGCATGGC
TTATAACCTACTTATCCACA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQSIEDIWQETLQIVKKNMSKPSYDTWMKSTTAHSLEGNTFIISAPNNFVRDWLEKSYTQFIANILQEITGRLFDVRFID
GEQEENFEYTVIKPNPALDEDGVEIGKHMLNPRYVFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLM
HAVGHYVQQHKDNAKVMYLSSEKFTNEFISSIRDNKTEEFRTKYRNVDVLLIDDIQFLAGKEGTQEEFFHTFNTLYDEQK
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKADGLDIPNEVMLYIANQIDSNIRELEGALIR
VVAYSSLVNKDITAGLAAEALKDIIPSSKSQVITISGIQEAVGEYFHVRLEDFKAKKRTKSIAFPRQIAMYLSRELTDAS
LPKIGDEFGGRDHTTVIHAHEKISQLLKTDQVLKNDLAEIEKNLRKAQNMF

Sequences:

>Translated_451_residues
MQSIEDIWQETLQIVKKNMSKPSYDTWMKSTTAHSLEGNTFIISAPNNFVRDWLEKSYTQFIANILQEITGRLFDVRFID
GEQEENFEYTVIKPNPALDEDGVEIGKHMLNPRYVFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLM
HAVGHYVQQHKDNAKVMYLSSEKFTNEFISSIRDNKTEEFRTKYRNVDVLLIDDIQFLAGKEGTQEEFFHTFNTLYDEQK
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKADGLDIPNEVMLYIANQIDSNIRELEGALIR
VVAYSSLVNKDITAGLAAEALKDIIPSSKSQVITISGIQEAVGEYFHVRLEDFKAKKRTKSIAFPRQIAMYLSRELTDAS
LPKIGDEFGGRDHTTVIHAHEKISQLLKTDQVLKNDLAEIEKNLRKAQNMF
>Mature_451_residues
MQSIEDIWQETLQIVKKNMSKPSYDTWMKSTTAHSLEGNTFIISAPNNFVRDWLEKSYTQFIANILQEITGRLFDVRFID
GEQEENFEYTVIKPNPALDEDGVEIGKHMLNPRYVFDTFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLM
HAVGHYVQQHKDNAKVMYLSSEKFTNEFISSIRDNKTEEFRTKYRNVDVLLIDDIQFLAGKEGTQEEFFHTFNTLYDEQK
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKADGLDIPNEVMLYIANQIDSNIRELEGALIR
VVAYSSLVNKDITAGLAAEALKDIIPSSKSQVITISGIQEAVGEYFHVRLEDFKAKKRTKSIAFPRQIAMYLSRELTDAS
LPKIGDEFGGRDHTTVIHAHEKISQLLKTDQVLKNDLAEIEKNLRKAQNMF

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=463, Percent_Identity=42.7645788336933, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI226510964, Length=198, Percent_Identity=25.2525252525253, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 51368; Mature: 51368

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSIEDIWQETLQIVKKNMSKPSYDTWMKSTTAHSLEGNTFIISAPNNFVRDWLEKSYTQ
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
FIANILQEITGRLFDVRFIDGEQEENFEYTVIKPNPALDEDGVEIGKHMLNPRYVFDTFV
HHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEE
IGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLMHAVGHYVQQHKDNAKVMYLS
ECCCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
SEKFTNEFISSIRDNKTEEFRTKYRNVDVLLIDDIQFLAGKEGTQEEFFHTFNTLYDEQK
CHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHCCC
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKADGLDIPNEVM
EEEEECCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
LYIANQIDSNIRELEGALIRVVAYSSLVNKDITAGLAAEALKDIIPSSKSQVITISGIQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHH
AVGEYFHVRLEDFKAKKRTKSIAFPRQIAMYLSRELTDASLPKIGDEFGGRDHTTVIHAH
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCEEEEHH
EKISQLLKTDQVLKNDLAEIEKNLRKAQNMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQSIEDIWQETLQIVKKNMSKPSYDTWMKSTTAHSLEGNTFIISAPNNFVRDWLEKSYTQ
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
FIANILQEITGRLFDVRFIDGEQEENFEYTVIKPNPALDEDGVEIGKHMLNPRYVFDTFV
HHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEE
IGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLMHAVGHYVQQHKDNAKVMYLS
ECCCCCHHHHHHHHHHHCCHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
SEKFTNEFISSIRDNKTEEFRTKYRNVDVLLIDDIQFLAGKEGTQEEFFHTFNTLYDEQK
CHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHCCC
QIIISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKADGLDIPNEVM
EEEEECCCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
LYIANQIDSNIRELEGALIRVVAYSSLVNKDITAGLAAEALKDIIPSSKSQVITISGIQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHH
AVGEYFHVRLEDFKAKKRTKSIAFPRQIAMYLSRELTDASLPKIGDEFGGRDHTTVIHAH
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCEEEEHH
EKISQLLKTDQVLKNDLAEIEKNLRKAQNMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]