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The map label for this gene is dnaN

Identifier: 226222641

GI number: 226222641

Start: 1868

End: 3013

Strand: Direct

Name: dnaN

Synonym: Lm4b_00002

Alternate gene names: 226222641

Gene position: 1868-3013 (Clockwise)

Preceding gene: 226222640

Following gene: 226222642

Centisome position: 0.06

GC content: 34.29

Gene sequence:

>1146_bases
ATGAAATTTGTTATTGAGCGTGATCGTCTTGTCCAAGCAGTCAATGAAGTTACTCGTGCCATCTCTGCAAGAACAACGAT
TCCAATTTTAACGGGGATAAAAATAGTCGTAAATGATGAAGGTGTAACACTAACTGGTAGTGATTCCGATATTTCAATCG
AAGCATTTATTCCATTAATTGAAAATGATGAAGTAATTGTAGAAGTGGAGAGTTTTGGTGGAATTGTACTTCAATCAAAA
TACTTTGGCGATATTGTTCGTCGTTTACCAGAAGAAAATGTAGAAATTGAAGTGACTTCTAACTACCAAACCAACATTAG
TTCTGGCCAAGCATCCTTTACATTAAATGGCTTAGATCCAATGGAATATCCTAAATTACCTGAAGTAACAGACGGAAAAA
CAATTAAAATTCCAATTAATGTACTTAAAAATATTGTTAGACAAACTGTTTTTGCTGTGTCTGCGATTGAAGTTCGTCCA
GTACTTACTGGTGTAAACTGGATTATCAAAGAAAATAAACTAAGCGCAGTTGCAACCGATAGTCATCGTCTAGCTTTACG
TGAAATACCTCTTGAAACAGACATTGATGAAGAATACAACATTGTTATTCCTGGAAAAAGTTTATCTGAATTAAATAAAC
TTTTAGATGATGCAAGCGAATCTATTGAAATGACCCTTGCCAACAACCAAATTCTTTTTAAATTAAAAGATTTATTATTT
TATTCTCGTTTACTTGAAGGTAGTTATCCAGATACATCTCGATTAATTCCAACTGATACTAAATCAGAATTAGTCATTAA
TTCCAAAGCATTTTTACAAGCAATTGACCGTGCGTCCCTACTTGCTCGCGAAAATCGTAATAACGTTATTAAATTAATGA
CGCTAGAAAATGGCCAAGTAGAAGTATCATCCAATTCTCCAGAAGTTGGGAATGTTTCTGAAAATGTCTTCAGCCAAAGT
TTTACTGGCGAAGAGATCAAAATATCTTTTAACGGTAAATACATGATGGATGCCCTACGTGCTTTTGAAGGTGATGATAT
TCAAATTTCCTTCTCCGGTACAATGAGACCATTCGTACTTCGACCAAAAGATGCAGCCAATCCAAATGAAATTTTACAAT
TAATCACGCCGGTTAGAACTTACTAA

Upstream 100 bases:

>100_bases
ATCCACAAATCCACAGCGCCTATTACTATTACTACGATTTTTTATTAATTAATTAAAGGTTTTTGAGGTTTTACAAAATA
TAAAAAAATGGGGGATACTC

Downstream 100 bases:

>100_bases
TCTCGCAAAAAGCACATCCGTCTTGGTTTCAAGGCGGATTCTTTTTTTGTTATAGTACAGTTTTTGGATAGAAATGTATA
TTTATGCAAACGGAGGTAAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKFVIERDRLVQAVNEVTRAISARTTIPILTGIKIVVNDEGVTLTGSDSDISIEAFIPLIENDEVIVEVESFGGIVLQSK
YFGDIVRRLPEENVEIEVTSNYQTNISSGQASFTLNGLDPMEYPKLPEVTDGKTIKIPINVLKNIVRQTVFAVSAIEVRP
VLTGVNWIIKENKLSAVATDSHRLALREIPLETDIDEEYNIVIPGKSLSELNKLLDDASESIEMTLANNQILFKLKDLLF
YSRLLEGSYPDTSRLIPTDTKSELVINSKAFLQAIDRASLLARENRNNVIKLMTLENGQVEVSSNSPEVGNVSENVFSQS
FTGEEIKISFNGKYMMDALRAFEGDDIQISFSGTMRPFVLRPKDAANPNEILQLITPVRTY

Sequences:

>Translated_381_residues
MKFVIERDRLVQAVNEVTRAISARTTIPILTGIKIVVNDEGVTLTGSDSDISIEAFIPLIENDEVIVEVESFGGIVLQSK
YFGDIVRRLPEENVEIEVTSNYQTNISSGQASFTLNGLDPMEYPKLPEVTDGKTIKIPINVLKNIVRQTVFAVSAIEVRP
VLTGVNWIIKENKLSAVATDSHRLALREIPLETDIDEEYNIVIPGKSLSELNKLLDDASESIEMTLANNQILFKLKDLLF
YSRLLEGSYPDTSRLIPTDTKSELVINSKAFLQAIDRASLLARENRNNVIKLMTLENGQVEVSSNSPEVGNVSENVFSQS
FTGEEIKISFNGKYMMDALRAFEGDDIQISFSGTMRPFVLRPKDAANPNEILQLITPVRTY
>Mature_381_residues
MKFVIERDRLVQAVNEVTRAISARTTIPILTGIKIVVNDEGVTLTGSDSDISIEAFIPLIENDEVIVEVESFGGIVLQSK
YFGDIVRRLPEENVEIEVTSNYQTNISSGQASFTLNGLDPMEYPKLPEVTDGKTIKIPINVLKNIVRQTVFAVSAIEVRP
VLTGVNWIIKENKLSAVATDSHRLALREIPLETDIDEEYNIVIPGKSLSELNKLLDDASESIEMTLANNQILFKLKDLLF
YSRLLEGSYPDTSRLIPTDTKSELVINSKAFLQAIDRASLLARENRNNVIKLMTLENGQVEVSSNSPEVGNVSENVFSQS
FTGEEIKISFNGKYMMDALRAFEGDDIQISFSGTMRPFVLRPKDAANPNEILQLITPVRTY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=353, Percent_Identity=28.0453257790368, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42430; Mature: 42430

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVIERDRLVQAVNEVTRAISARTTIPILTGIKIVVNDEGVTLTGSDSDISIEAFIPLI
CCEEECHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCEEEECCCCCEEEEEEEEEE
ENDEVIVEVESFGGIVLQSKYFGDIVRRLPEENVEIEVTSNYQTNISSGQASFTLNGLDP
CCCEEEEEEECCCCEEEEHHHHHHHHHHCCCCCEEEEEECCCEECCCCCCEEEEECCCCC
MEYPKLPEVTDGKTIKIPINVLKNIVRQTVFAVSAIEVRPVLTGVNWIIKENKLSAVATD
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEECCCEEEEECC
SHRLALREIPLETDIDEEYNIVIPGKSLSELNKLLDDASESIEMTLANNQILFKLKDLLF
CCEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCEEEEHHHHHHH
YSRLLEGSYPDTSRLIPTDTKSELVINSKAFLQAIDRASLLARENRNNVIKLMTLENGQV
HHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEE
EVSSNSPEVGNVSENVFSQSFTGEEIKISFNGKYMMDALRAFEGDDIQISFSGTMRPFVL
EECCCCCCCCCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEECCCCCEEEE
RPKDAANPNEILQLITPVRTY
CCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFVIERDRLVQAVNEVTRAISARTTIPILTGIKIVVNDEGVTLTGSDSDISIEAFIPLI
CCEEECHHHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCEEEECCCCCEEEEEEEEEE
ENDEVIVEVESFGGIVLQSKYFGDIVRRLPEENVEIEVTSNYQTNISSGQASFTLNGLDP
CCCEEEEEEECCCCEEEEHHHHHHHHHHCCCCCEEEEEECCCEECCCCCCEEEEECCCCC
MEYPKLPEVTDGKTIKIPINVLKNIVRQTVFAVSAIEVRPVLTGVNWIIKENKLSAVATD
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEEECCEEEEECCCEEEEECC
SHRLALREIPLETDIDEEYNIVIPGKSLSELNKLLDDASESIEMTLANNQILFKLKDLLF
CCEEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEEEEEECCEEEEHHHHHHH
YSRLLEGSYPDTSRLIPTDTKSELVINSKAFLQAIDRASLLARENRNNVIKLMTLENGQV
HHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEE
EVSSNSPEVGNVSENVFSQSFTGEEIKISFNGKYMMDALRAFEGDDIQISFSGTMRPFVL
EECCCCCCCCCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCEEEEECCCCCEEEE
RPKDAANPNEILQLITPVRTY
CCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]