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The map label for this gene is phoB [C]

Identifier: 225862009

GI number: 225862009

Start: 2065890

End: 2066543

Strand: Reverse

Name: phoB [C]

Synonym: SPT_2211

Alternate gene names: 225862009

Gene position: 2066543-2065890 (Counterclockwise)

Preceding gene: 225862011

Following gene: 225862008

Centisome position: 97.84

GC content: 36.39

Gene sequence:

>654_bases
ATGAACATTTTAGTTGCAGATGACGAGGAAATGATTAGAGAAGGAATTGCAGCATTTCTGACAGAAGAGGGTTATCATGT
CATTATGGCTAAGGATGGACAAGAGGTCTTGGAAAAATTTCAAGATCTCCCTATCCATCTCATGGTACTGGATTTAATGA
TGCCTAGGAAGAGTGGTTTTGAAGTGTTAAAAGAAATCAATCAAAAGCACGATATTCCTGTCATCGTCTTGAGTGCTCTG
GGAGATGAAACTACTCAGTCACAGGTATTTGATCTCTATGCTGATGATCATGTGACAAAACCTTTTTCTTTGGTACTGCT
TGTCAAGCGTATTAAGGCGCTTATCAGACGTTACTACGTCATAGAGGATCTTTGGCGATATCAGGATGTAACAGTGGATT
TTACCTCTTACAAAGCACATTATAAAAATGAAGAAATTGATCTCAAACCAAAGGAATTACTGGTACTAAAGTGTTTGATT
CAGCATAAAAATCAAGTTTTAAGTAGAGAGCAGATATTGGAAGAAATTTCAAAAGATGTAGCTGATTTACCTTGTGATAG
GGTCGTTGATGTCTATATTCGTACTCTTCGCAAAAAATTAGCTTTAGATTGTATCGTGACTGTGAAAAATGTTGGGTATA
AGATTAGCTTATGA

Upstream 100 bases:

>100_bases
GAAATCCATTCCATTATCATAGATGATAGGCTGGTAGGAAATTTTCAAATAGCATACAGGAAATAGATGTATGGAGTTCT
GGTAGTAGAAAGGGAGAGAG

Downstream 100 bases:

>100_bases
TAAAAAATCCTAAATTATTAACCAAGTCTTTTTTAAGAAGTTTTGCAATTCTAGGTGGTGTTGGTCTAGTCATTCATATA
GCTATTTATTTGACCTTTCC

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLPIHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSAL
GDETTQSQVFDLYADDHVTKPFSLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL

Sequences:

>Translated_217_residues
MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLPIHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSAL
GDETTQSQVFDLYADDHVTKPFSLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL
>Mature_217_residues
MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLPIHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSAL
GDETTQSQVFDLYADDHVTKPFSLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL

Specific function: This Protein Is A Positive Regulator For The Phosphate Regulon. Transcription Of This Operon Is Positively Regulated By Phob And Phor When Phosphate Is Limited. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=223, Percent_Identity=34.0807174887892, Blast_Score=123, Evalue=7e-30,
Organism=Escherichia coli, GI1786911, Length=224, Percent_Identity=30.3571428571429, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789402, Length=218, Percent_Identity=31.1926605504587, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788394, Length=222, Percent_Identity=31.0810810810811, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=34.1880341880342, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787229, Length=220, Percent_Identity=27.2727272727273, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1786784, Length=223, Percent_Identity=30.0448430493274, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=29.1479820627803, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1789809, Length=225, Percent_Identity=28.4444444444444, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082012, Length=218, Percent_Identity=27.9816513761468, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790552, Length=218, Percent_Identity=30.7339449541284, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=24.0174672489083, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=25.5605381165919, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI145693140, Length=229, Percent_Identity=26.2008733624454, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25205; Mature: 25205

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLPIHLMVLDLMMPRKSGF
CCEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCHH
EVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPFSLVLLVKRIKALIRRYYV
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLIQHKNQVLSREQILEEISKDV
HHHHHHHHCCEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
>Mature Secondary Structure
MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLPIHLMVLDLMMPRKSGF
CCEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCHHHHHHHHHCCCCCHH
EVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPFSLVLLVKRIKALIRRYYV
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
IEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLIQHKNQVLSREQILEEISKDV
HHHHHHHHCCEEEHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1554697 [H]