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The map label for this gene is clpB [H]

Identifier: 225862011

GI number: 225862011

Start: 2067185

End: 2069617

Strand: Reverse

Name: clpB [H]

Synonym: SPT_2213

Alternate gene names: 225862011

Gene position: 2069617-2067185 (Counterclockwise)

Preceding gene: 225862012

Following gene: 225862009

Centisome position: 97.99

GC content: 45.5

Gene sequence:

>2433_bases
ATGAACTATTCAAAAGCATTGAATGAATGTATCGAAAGTGCCTACATGGTTGCTGGACATTTTGGAGCTCGTTATCTAGA
GTCTTGGCACTTGTTGATTGCCATGTCTAATCACAGTTATAGTGTAGCAGGGGCAACTTTAAATGATTATCCGTATGAGA
TGGACCGTTTAGAAGAGGTGGCTTTGGAACTGACTGAAACGGACTATAGCCAGGATGAAACCTTTACGGAATTGCCGTTC
TCCCGTCGTTTGCAGGTTCTTTTTGATGAAGCAGAGTATGTAGCGTCAGTGGTCCATGCTAAGGTACTAGGGACAGAGCA
CGTCCTCTATGCGATTTTGCATGATGGCAATGCCTTGGCGACTCGTATCTTGGAGAGGGCTGGTTTTTCTTATGAAGACA
AGAAAGATCAGGTCAAGATTGCTGCTCTTCGTCGAAATTTAGAAGAACGGGCAGGCTGGACTCGTGAAGATCTCAAGGCT
TTACGCCAACGCCATCGTACAGTAGCTGACAAGCAAAATTCTATGGCCAATATGATGGGCATGCCGCAGACTCCTAGTGG
TGGTCTCGAGGACTATACGCATGATTTGACAGAGCAAGCGCGTTCTGGCAAGTTAGAACCAGTCATCGGTCGGGACAAGG
AAATCTCACGTATGATTCAAATCTTGAGCCGTAAGACTAAGAACAACCCTGTCTTGGTTGGGGATGCTGGTGTCGGGAAA
ACAGCTCTGGCGCTTGGTCTTGCTCAGCGTATTGCTAGTGGTGACGTGCCTGCGGAAATGGCTAAGATGCGCGTGTTAGA
ACTTGATTTGATGAATGTCGTTGCAGGGACACGCTTCCGTGGTGACTTTGAAGAACGCATGAATAATATCATCAAGGATA
TTGAAGAAGATGGCCAAGTCATCCTCTTTATCGATGAACTTCACACCATCATGGGTTCTGGTAGCGGGATTGATTCGACT
CTGGATGCGGCCAATATCTTGAAACCAGCCTTGGCGCGTGGAACTTTGAGAACGGTTGGTGCCACCACTCAGGAAGAATA
TCAAAAACATATCGAAAAAGATGCAGCACTTTCTCGTCGTTTCGCTAAAGTGACGATTGAAGAACCAAGTGTGGCAGATA
GTATGACTATTTTACAAGGTTTGAAGGCGACTTATGAGAAACATCACCGTGTACAAATCACAGATGAAGCGGTTGAAACA
GCGGTTAAGATGGCTCATCGTTATTTAACCAGTCGTCACTTGCCAGACTCTGCTATCGATCTCTTGGATGAGGCGGCAGC
AACAGTGCAAAATAAATCAAAGCATGTAAAAGCAGACGATTCTGACTTGAGTCCAGCTGACAAGGCCTTGATGGATGGCA
AGTGGAAACAGGCAGCCCAGCTAATCGCAAAAGAAGAGGAAGTGCCTGTCTATAAAGACTTGGTGACAGAATCTGATATT
TTGACCACCTTGAGTCGCTTGTCAGGTATCCCCGTTCAAAAACTGACTCAGACTGATGCCAAGAAATACTTAAACTTGGA
AGCTGAACTGCACAAACGTGTCATCGGTCAAGACCAAGCTGTTTCAAGTATTAGTCGTGCCATTCGCCGCAATCAGTCAG
GAATTCGCAGTCACAAGCGTCCGATCGGTTCCTTTATGTTCCTAGGACCCACAGGTGTCGGTAAGACCGAGTTGGCCAAG
GCTTTGGCAGAAGTTCTCTTTGATGACGAATCAGCCCTTATCCGCTTTGATATGAGTGAGTATATGGAGAAATTCGCAGC
CAGCCGTCTAAATGGAGCTCCTCCAGGCTATGTGGGTTACGAAGAAGGTGGGGAGTTGACAGAGAAGGTTCGCAATAAAC
CCTATTCCGTTCTCCTCTTTGATGAGGTAGAGAAGGCCCATCCAGATATCTTTAATGTTCTCTTGCAGGTTCTGGATGAC
GGTGTCTTGACAGATAGCAAGGGACGCAAGGTCGATTTTTCAAATACCATTATCATTATGACATCGAACTTAGGTGCGAC
TGCCCTTCGTGATGATAAGACTGTTGGTTTTGGGGCTAAGGATATTCGTTTTGACCAGGAAAATATGGAAAAACGCATGT
TTGAAGAACTGAAAAAAGCTTATAGACCGGAATTCATCAACCGTATTGATGAGAAGGTGGTCTTCCATAGCCTATCTAGT
GACCATATGCAGGAAGTGGTGAAGATTATGGTCAAGCCTTTAGTGGCAAGTTTGGCTGAAAAAGGCATTGACTTGAAATT
ACAAGCTTCAGCTCTGAAATTGTTAGCAAATCAAGGATATGACCCAGAGATGGGAGCTCGCCCACTTCGCAGAACCCTGC
AAACAGAAGTGGAGGACAAGTTGGCAGAACTTCTTCTTAAGGGAGATTTAGTGGCAGGCAGCACACTTAAGATTGGTGTC
AAAGCAGGCCAGTTAAAATTTGATATTGCATAA

Upstream 100 bases:

>100_bases
TCAGTAGCTTTGGATCGCGTTTTAGGAGAAGAAGCTCCAGTTGTTCGAGCAAACATGCTACGTCAGATCATACAAGAAGT
AGATAGAAAAGGGAAGTAAG

Downstream 100 bases:

>100_bases
AAGAATAAAAGTATCAGCATCTGACCATAAGTCACAGTGGAGTGAAACTCAATGAAAATCAAAGAGCAAACTAGGCAGCT
AGCCGCAGGTTGCTCAAAAC

Product: chaperone ClpB 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 810; Mature: 810

Protein sequence:

>810_residues
MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAGATLNDYPYEMDRLEEVALELTETDYSQDETFTELPF
SRRLQVLFDEAEYVASVVHAKVLGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRMIQILSRKTKNNPVLVGDAGVGK
TALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAGTRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDST
LDAANILKPALARGTLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQITDEAVET
AVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKALMDGKWKQAAQLIAKEEEVPVYKDLVTESDI
LTTLSRLSGIPVQKLTQTDAKKYLNLEAELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAK
ALAEVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVEKAHPDIFNVLLQVLDD
GVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDIRFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSS
DHMQEVVKIMVKPLVASLAEKGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKIGV
KAGQLKFDIA

Sequences:

>Translated_810_residues
MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAGATLNDYPYEMDRLEEVALELTETDYSQDETFTELPF
SRRLQVLFDEAEYVASVVHAKVLGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRMIQILSRKTKNNPVLVGDAGVGK
TALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAGTRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDST
LDAANILKPALARGTLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQITDEAVET
AVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKALMDGKWKQAAQLIAKEEEVPVYKDLVTESDI
LTTLSRLSGIPVQKLTQTDAKKYLNLEAELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAK
ALAEVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVEKAHPDIFNVLLQVLDD
GVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDIRFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSS
DHMQEVVKIMVKPLVASLAEKGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKIGV
KAGQLKFDIA
>Mature_810_residues
MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAGATLNDYPYEMDRLEEVALELTETDYSQDETFTELPF
SRRLQVLFDEAEYVASVVHAKVLGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRMIQILSRKTKNNPVLVGDAGVGK
TALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAGTRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDST
LDAANILKPALARGTLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQITDEAVET
AVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKALMDGKWKQAAQLIAKEEEVPVYKDLVTESDI
LTTLSRLSGIPVQKLTQTDAKKYLNLEAELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAK
ALAEVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVEKAHPDIFNVLLQVLDD
GVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDIRFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSS
DHMQEVVKIMVKPLVASLAEKGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKIGV
KAGQLKFDIA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=346, Percent_Identity=33.2369942196532, Blast_Score=182, Evalue=8e-46,
Organism=Escherichia coli, GI1787109, Length=641, Percent_Identity=42.4336973478939, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI1788943, Length=331, Percent_Identity=52.5679758308157, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6320464, Length=340, Percent_Identity=49.4117647058824, Blast_Score=327, Evalue=7e-90,
Organism=Saccharomyces cerevisiae, GI6323002, Length=333, Percent_Identity=43.5435435435435, Blast_Score=271, Evalue=4e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 90171; Mature: 90171

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAGATLNDYPYEMDRLEEV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHH
ALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKVLGTEHVLYAILHDGNALA
HHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEECCHHHH
TRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKALRQRHRTVADKQNSMANMMG
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
MPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRMIQILSRKTKNNPVLVGDAGVGK
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCH
TALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAGTRFRGDFEERMNNIIKDIEEDGQV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCE
ILFIDELHTIMGSGSGIDSTLDAANILKPALARGTLRTVGATTQEEYQKHIEKDAALSRR
EEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHH
FAKVTIEEPSVADSMTILQGLKATYEKHHRVQITDEAVETAVKMAHRYLTSRHLPDSAID
HHEEEECCCCHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHH
LLDEAAATVQNKSKHVKADDSDLSPADKALMDGKWKQAAQLIAKEEEVPVYKDLVTESDI
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
LTTLSRLSGIPVQKLTQTDAKKYLNLEAELHKRVIGQDQAVSSISRAIRRNQSGIRSHKR
HHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHCC
PIGSFMFLGPTGVGKTELAKALAEVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGY
CCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCC
EEGGELTEKVRNKPYSVLLFDEVEKAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIM
CCCCHHHHHHCCCCCEEEEEHHHHHHCHHHHHHHHHHHCCCCEECCCCCEEECCCEEEEE
TSNLGATALRDDKTVGFGAKDIRFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSS
ECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCH
DHMQEVVKIMVKPLVASLAEKGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDK
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LAELLLKGDLVAGSTLKIGVKAGQLKFDIA
HHHHHHHCCCCCCCEEEEEEEECCEEEECC
>Mature Secondary Structure
MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAGATLNDYPYEMDRLEEV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHH
ALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKVLGTEHVLYAILHDGNALA
HHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEECCHHHH
TRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKALRQRHRTVADKQNSMANMMG
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
MPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRMIQILSRKTKNNPVLVGDAGVGK
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCH
TALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAGTRFRGDFEERMNNIIKDIEEDGQV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCE
ILFIDELHTIMGSGSGIDSTLDAANILKPALARGTLRTVGATTQEEYQKHIEKDAALSRR
EEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHH
FAKVTIEEPSVADSMTILQGLKATYEKHHRVQITDEAVETAVKMAHRYLTSRHLPDSAID
HHEEEECCCCHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHH
LLDEAAATVQNKSKHVKADDSDLSPADKALMDGKWKQAAQLIAKEEEVPVYKDLVTESDI
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
LTTLSRLSGIPVQKLTQTDAKKYLNLEAELHKRVIGQDQAVSSISRAIRRNQSGIRSHKR
HHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHCC
PIGSFMFLGPTGVGKTELAKALAEVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGY
CCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCC
EEGGELTEKVRNKPYSVLLFDEVEKAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIM
CCCCHHHHHHCCCCCEEEEEHHHHHHCHHHHHHHHHHHCCCCEECCCCCEEECCCEEEEE
TSNLGATALRDDKTVGFGAKDIRFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSS
ECCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCH
DHMQEVVKIMVKPLVASLAEKGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDK
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
LAELLLKGDLVAGSTLKIGVKAGQLKFDIA
HHHHHHHCCCCCCCEEEEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]