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The map label for this gene is pbpF [H]

Identifier: 225861915

GI number: 225861915

Start: 1957580

End: 1960045

Strand: Reverse

Name: pbpF [H]

Synonym: SPT_2108

Alternate gene names: 225861915

Gene position: 1960045-1957580 (Counterclockwise)

Preceding gene: 225861917

Following gene: 225861914

Centisome position: 92.8

GC content: 42.7

Gene sequence:

>2466_bases
ATGCAAAATCAATTAAATGAATTAAAACGAAAAATGCTGGAATTTTTCCAGCAAAAACAAAAAAATAAAAAATCAGCTAG
ACCTGGCAAGAAAGGTTCAAGTACCAAAAAATCTAAAACCTTAGATAAGTCAGCCATTTTCCCAGCTATTTTACTGAGTA
TAAAAGCCTTATTTAATTTACTCTTTGTACTCGGTTTTCTAGGAGGAATGTTGGGAGTTGGGATTGCTTTGGGGTACGGA
GTGGCCTTATTTGACAAGGTTCGGGTGCCTCAGACAGAAGAATTGGTGAATCAGGTCAAGGACATCTCTTCTATTTCAGA
GATTACCTATTCGGACGGGACGGTGATTGCTTCCATAGAGAGTGATTTGTTGCGCACTTCTATCTCATCTGAGCAAATTT
CGGAAAATCTGAAGAAGGCTATCATTGCGACAGAAGATGAACACTTTAAAGAACATAAGGGTGTAGTACCCAAGGCGGTG
ATTCGTGCGACCTTGGGGAAATTTGTAGGTTTGGGTTCCTCTAGTGGGGGTTCAACCTTGACCCAGCAACTAATTAAACA
GCAGGTTGTTGGGGATGCGCCGACCTTGGCTCGTAAGGCGGCAGAGATTGTGGATGCTCTTGCCTTGGAACGCGCCATGA
ATAAAGATGAGATTTTAACGACCTATCTCAATGTGGCTCCCTTTGGCCGAAATAATAAGGGACAGAATATTGCAGGGGCT
CGGCAAGCAGCTGAGGGAATTTTCGGTGTAGATGCCAGTCAGTTGACTGTTCCTCAAGCAGCATTTTTAGCAGGACTTCC
ACAGAGTCCCATTACTTACTCTCCTTATGAAAATACTGGGGAGTTGAAGAGTGATGAAGACCTAGAAATTGGCTTAAGAC
GGGCTAAGGCAGTTCTTTACAGTATGTATCGTACAGGTGCATTAAGCAAAGACGAGTATTCTCAGTACAAGGATTATGAC
CTTAAACAGGACTTTTTACCATCGGGCACGGTTACAGGAATTTCACGAGACTATTTATACTTTACAACTTTGGCAGAAGC
TCAAGAACGTATGTATGACTATCTAGCTCAGAGAGACAATGTCTCCGCTAAGGAGTTGAAAAATGAGGTAACTCAGAAGT
TTTATCGAGATTTGGCAGCCAAGGAAATTGAAAATGGTGGTTATAAGATTACTACTACCATAGATCAGAAAATTCATTCT
GCCATGCAAAGTGCGGTTGCTGATTATGGCTATCTTTTAGACGATGGAACAGGTCGTGTAGAAGTAGGGAATGTCTTGAT
GGACAACCAAACAGGTGCTATTCTAGGCTTTGTAGGTGGTCGTAATTATCAAGAAAATCAAAATAATCATGCCTTTGATA
CCAAACGTTCGCCAGCTTCTACTACCAAGCCCTTGCTGGCCTACGGTATTGCTATTGACCAGGGCTTGATGGGAAGTGAA
ACGATTCTATCTAACTATCCAACAAACTTTGCTAATGGCAATCCGATTATGTATGCTAATAGCAAGGGAACAGGAATGAT
GACCTTGGGAGAAGCTCTGAACTATTCATGGAATATCCCTGCTTACTGGACCTATCGTATGCTCCGTGAAAAGGGTGTTG
ATGTCAAGGGTTATATGGAAAAGATGGGTTACGAGATTCCTGAGTACGGTATTGAGAGCTTGCCAATGGGTGGTGGTATT
GAAGTCACAGTTGCCCAGCATACCAATGGCTATCAGACCTTAGCTAATAATGGAGTTTATCATCAGAAGCATGTGATTTC
AAAGATTGAAGCAGCAGATGGTAGAGTGGTGTATGAGTATCAGGATAAACCGGTTCAAGTCTATTCAAAAGCTACTGCGA
CGATTATGCAGGGATTGCTACGAGAAGTTCTATCCTCTCGTGTGACAACAACCTTCAAGTCTAACCTGACTTCTTTAAAT
CCTACTCTGGCTAATGCAGATTGGATTGGGAAGACTGGTACAACCAACCAAGACGAAAATATGTGGCTCATGCTTTCGAC
ACCTAGATTAACCCTAGGTGGCTGGATTGGGCATGATGATAATCATTCATTGTCACGTAGAGCAGGTTATTCTAATAACT
CTAATTACATGGCTCATCTGGTAAATGCGATTCAGCAAGCTTCCCCAAGCATTTGGGGGAACGAGCGCTTTGCTTTAGAT
CCTAGTGTAGTGAAATCGGAAGTCTTGAAATCAACAGGTCAAAAACCAGGGAAGGTTTCTGTTGAAGGAAAAGAAGTAGA
GGTCACAGGTTCGACTGTTACCAGCTATTGGGCTAATAAGTCAGGAGCGCCAGCGACAAGTTATCGCTTTGCTATTGGCG
GAAGTGATGCGGATTATCAGAATGCTTGGTCTAGTATTGTGGGGAGTCTACCAACTCCATCCAGCTCCAGCAGTTCAAGT
AGTAGTTCTAGCGATAGCAGTAACTCAAGTACTACACGACCTTCTTCTTCAAGGGCGAGACGATAA

Upstream 100 bases:

>100_bases
TATCACAAACTTGGGCAATAGCCTAGCAGAATAGGGAAGAAAGTGGCTATTTTATTGAAAATTTCCCTTTTGTGGTATAA
TAGATAAAGTGAGGAAATCC

Downstream 100 bases:

>100_bases
TTCTCTAAATGAAGTGGCCAATCAATGGATTGCCACTTTTTTCCTTTCGTGTTACAATAAGGGTATGAATCAGTATCAGA
AAAAGATTGTTAACGGAAAA

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 821; Mature: 821

Protein sequence:

>821_residues
MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKSAIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYG
VALFDKVRVPQTEELVNQVKDISSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEILTTYLNVAPFGRNNKGQNIAGA
RQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPYENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYD
LKQDFLPSGTVTGISRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEVTQKFYRDLAAKEIENGGYKITTTIDQKIHS
AMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNHAFDTKRSPASTTKPLLAYGIAIDQGLMGSE
TILSNYPTNFANGNPIMYANSKGTGMMTLGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGI
EVTVAQHTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVLSSRVTTTFKSNLTSLN
PTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNHSLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALD
PSVVKSEVLKSTGQKPGKVSVEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSSSS
SSSSDSSNSSTTRPSSSRARR

Sequences:

>Translated_821_residues
MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKSAIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYG
VALFDKVRVPQTEELVNQVKDISSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEILTTYLNVAPFGRNNKGQNIAGA
RQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPYENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYD
LKQDFLPSGTVTGISRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEVTQKFYRDLAAKEIENGGYKITTTIDQKIHS
AMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNHAFDTKRSPASTTKPLLAYGIAIDQGLMGSE
TILSNYPTNFANGNPIMYANSKGTGMMTLGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGI
EVTVAQHTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVLSSRVTTTFKSNLTSLN
PTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNHSLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALD
PSVVKSEVLKSTGQKPGKVSVEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSSSS
SSSSDSSNSSTTRPSSSRARR
>Mature_821_residues
MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKSAIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYG
VALFDKVRVPQTEELVNQVKDISSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEILTTYLNVAPFGRNNKGQNIAGA
RQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPYENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYD
LKQDFLPSGTVTGISRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEVTQKFYRDLAAKEIENGGYKITTTIDQKIHS
AMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNHAFDTKRSPASTTKPLLAYGIAIDQGLMGSE
TILSNYPTNFANGNPIMYANSKGTGMMTLGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGI
EVTVAQHTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVLSSRVTTTFKSNLTSLN
PTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNHSLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALD
PSVVKSEVLKSTGQKPGKVSVEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSSSS
SSSSDSSNSSTTRPSSSRARR

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=346, Percent_Identity=28.9017341040462, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1788867, Length=613, Percent_Identity=23.1647634584013, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI1786343, Length=590, Percent_Identity=23.3898305084746, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1789601, Length=246, Percent_Identity=29.6747967479675, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 89552; Mature: 89552

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKSAIFPAILLSIKALFNL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDISSISEITYSDGTVIASIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHH
SDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAVIRATLGKFVGLGSSSGGSTL
HHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHH
TQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEILTTYLNVAPFGRNNKGQNIAGA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHH
RQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPYENTGELKSDEDLEIGLRRAKAVLY
HHHHCCCCCCCHHHHCCCHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHH
SMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGISRDYLYFTTLAEAQERMYDYLAQRDN
HHHHHCCCCHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VSAKELKNEVTQKFYRDLAAKEIENGGYKITTTIDQKIHSAMQSAVADYGYLLDDGTGRV
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEECCCCCE
EVGNVLMDNQTGAILGFVGGRNYQENQNNHAFDTKRSPASTTKPLLAYGIAIDQGLMGSE
EECCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCH
TILSNYPTNFANGNPIMYANSKGTGMMTLGEALNYSWNIPAYWTYRMLREKGVDVKGYME
HHHHCCCCCCCCCCEEEEECCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHH
KMGYEIPEYGIESLPMGGGIEVTVAQHTNGYQTLANNGVYHQKHVISKIEAADGRVVYEY
HCCCCCCCCCHHHCCCCCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEEE
QDKPVQVYSKATATIMQGLLREVLSSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDEN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
MWLMLSTPRLTLGGWIGHDDNHSLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALD
EEEEEECCCEEECCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEC
PSVVKSEVLKSTGQKPGKVSVEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQ
HHHHHHHHHHHHCCCCCEEEECCCEEEEECCHHHHHHCCCCCCCCCCEEEEECCCCCHHH
NAWSSIVGSLPTPSSSSSSSSSSSDSSNSSTTRPSSSRARR
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKSAIFPAILLSIKALFNL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDISSISEITYSDGTVIASIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHH
SDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAVIRATLGKFVGLGSSSGGSTL
HHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHH
TQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEILTTYLNVAPFGRNNKGQNIAGA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHH
RQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPYENTGELKSDEDLEIGLRRAKAVLY
HHHHCCCCCCCHHHHCCCHHHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHH
SMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGISRDYLYFTTLAEAQERMYDYLAQRDN
HHHHHCCCCHHHHHHHCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VSAKELKNEVTQKFYRDLAAKEIENGGYKITTTIDQKIHSAMQSAVADYGYLLDDGTGRV
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEECCCCCE
EVGNVLMDNQTGAILGFVGGRNYQENQNNHAFDTKRSPASTTKPLLAYGIAIDQGLMGSE
EECCEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCH
TILSNYPTNFANGNPIMYANSKGTGMMTLGEALNYSWNIPAYWTYRMLREKGVDVKGYME
HHHHCCCCCCCCCCEEEEECCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHH
KMGYEIPEYGIESLPMGGGIEVTVAQHTNGYQTLANNGVYHQKHVISKIEAADGRVVYEY
HCCCCCCCCCHHHCCCCCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEEEE
QDKPVQVYSKATATIMQGLLREVLSSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDEN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
MWLMLSTPRLTLGGWIGHDDNHSLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALD
EEEEEECCCEEECCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEC
PSVVKSEVLKSTGQKPGKVSVEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQ
HHHHHHHHHHHHCCCCCEEEECCCEEEEECCHHHHHHCCCCCCCCCCEEEEECCCCCHHH
NAWSSIVGSLPTPSSSSSSSSSSSDSSNSSTTRPSSSRARR
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]