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The map label for this gene is dapL [H]

Identifier: 225861912

GI number: 225861912

Start: 1954478

End: 1955608

Strand: Reverse

Name: dapL [H]

Synonym: SPT_2105

Alternate gene names: 225861912

Gene position: 1955608-1954478 (Counterclockwise)

Preceding gene: 225861913

Following gene: 225861911

Centisome position: 92.59

GC content: 46.77

Gene sequence:

>1131_bases
ATGTTAGATTTGATTCAGACTAGACGAGATTTACACCAGATTCCAGAGATTGGCTTGGAGGAGTTCAAGACTCAGGCTTA
TTTGCTGGATGTGATTGAGAAATTGACTACGGGCAAGGATTTTGTTCAAATTCGTACTTGGCGGACAGGGATTTTGGTCT
ACCTGCAGGGAAGTCAGCCGGAGCGAACCATTGGTTGGCGGACAGATATTGATGGCCTGCCTATCGTCGAACAAACAGGT
CTACCTTTTGCTTCTCAACACCAAGGTCGCATGCATGCCTGTGGCCATGATTTTCATATGACCATTGCCTTAGGCTGTCT
TGAGCGCGCCCTTGAGGAGCAACCTAAAAATAATCTGCTCTTTCTATTTCAGCCTGCTGAAGAAAATGAAGCTGGTGGTA
TGCTCATGTATGAGGCTGGTGCTTTTGGAGATTGGTTGCCAGATCAATTTTATGGCCTCCATGTCCGTCCAGATTTGAAA
GTCGGCCAGATTGCGACTAATACTCATACACTCTTTGCAGGAACTTGCGAGGTGAAGATCCGTTTCAAAGGTAAAGGTGG
CCACGCGGCTTTTCCTCATGAAGCCAATGACGCCTTGGTGGCGGCTAGTTACTTTGTGACCCAAGTGCAGTCAGTTGTTA
GCCGCAATGTAAACCCAATCGAGGGAGCAGTGGTGACTTTCGGCGTTTTTCAAGCTGGAACCACCAACAATGTCATCACA
GACACAGCTTTTTTACATGGAACTATTCGTGCCTTGACACAGGACATGAGTCTCTTGGTGCAAAAGAGGGTAAAAACAGT
TGCAGAAGGGATTGCAGCTGCCTTTGATATGGAAGTCGAAGTGGAACTCAAACAAGGTGGATACCTACCTGTTGAGAACA
ATCCAGCCTTGGCGCGTGAACTGATGGACTTCTTTGACGAAAAAGACGGAATCGAGTTGATTGATATCGAACCTGCTATG
ACAGGCGAGGACTTTGGTTATCTCCTTTCAAAGGTTGATGGCGTGATGTTTTGGCTAGGTATCGATAGTCCCTACGCTCT
TCATCACCCTCAAATGAGTCCTAAGGAAGAAGTCTTAGCTATTGGGGTGGCTGCGGTGTCTAGTTTCCTGAAAAAGAAGG
CAGCAGAATAG

Upstream 100 bases:

>100_bases
ACAGCGTTAGAGGATGCGCTTCGTACCTTGTAATTGTAAAAGTAAAAAAGAGGCGGAACCCTTTTTCCAGCCTCTTTCTG
CTATATCGGAGGACAGATAG

Downstream 100 bases:

>100_bases
AGGGCTTGTCTATGAAATCGGAATTACGCAAGCAAGTCTTGCATGAAATGAAGGCTTTATCTCAAGAACAAAAACAGGCT
ATAGACCAAGCTTTAACCGA

Product: peptidase, M20/M25/M40 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQIRTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTG
LPFASQHQGRMHACGHDFHMTIALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLK
VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVNPIEGAVVTFGVFQAGTTNNVIT
DTAFLHGTIRALTQDMSLLVQKRVKTVAEGIAAAFDMEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAM
TGEDFGYLLSKVDGVMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE

Sequences:

>Translated_376_residues
MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQIRTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTG
LPFASQHQGRMHACGHDFHMTIALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLK
VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVNPIEGAVVTFGVFQAGTTNNVIT
DTAFLHGTIRALTQDMSLLVQKRVKTVAEGIAAAFDMEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAM
TGEDFGYLLSKVDGVMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE
>Mature_376_residues
MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQIRTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTG
LPFASQHQGRMHACGHDFHMTIALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLK
VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVNPIEGAVVTFGVFQAGTTNNVIT
DTAFLHGTIRALTQDMSLLVQKRVKTVAEGIAAAFDMEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAM
TGEDFGYLLSKVDGVMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE

Specific function: Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapL subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=414, Percent_Identity=25.1207729468599, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.47 [H]

Molecular weight: Translated: 41592; Mature: 41592

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQIRTWRTGILVYLQGSQP
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECEEEEEEECCCC
ERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTIALGCLERALEEQPKNNLL
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
FLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLKVGQIATNTHTLFAGTCEVKI
EEEECCCCCCCCCEEEEECCCCCCCCCCHHEEEEECCCCCCCEEECCCEEEEEEEEEEEE
RFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVNPIEGAVVTFGVFQAGTTNNVIT
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEE
DTAFLHGTIRALTQDMSLLVQKRVKTVAEGIAAAFDMEVEVELKQGGYLPVENNPALARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCHHHHHH
LMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDGVMFWLGIDSPYALHHPQMSPKEEVLA
HHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCEECCCCCCCHHHHHH
IGVAAVSSFLKKKAAE
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQIRTWRTGILVYLQGSQP
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECEEEEEEECCCC
ERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTIALGCLERALEEQPKNNLL
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCEE
FLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLKVGQIATNTHTLFAGTCEVKI
EEEECCCCCCCCCEEEEECCCCCCCCCCHHEEEEECCCCCCCEEECCCEEEEEEEEEEEE
RFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVNPIEGAVVTFGVFQAGTTNNVIT
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEE
DTAFLHGTIRALTQDMSLLVQKRVKTVAEGIAAAFDMEVEVELKQGGYLPVENNPALARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCCHHHHHH
LMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDGVMFWLGIDSPYALHHPQMSPKEEVLA
HHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCCEECCCCCCCHHHHHH
IGVAAVSSFLKKKAAE
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA