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The map label for this gene is hsdS [H]

Identifier: 225858346

GI number: 225858346

Start: 515163

End: 516713

Strand: Reverse

Name: hsdS [H]

Synonym: SP70585_0566

Alternate gene names: 225858346

Gene position: 516713-515163 (Counterclockwise)

Preceding gene: 225858347

Following gene: 225858343

Centisome position: 23.65

GC content: 31.27

Gene sequence:

>1551_bases
ATGACACCAGAACAACTTAAGGCAAGTATTCTCCAAAGAGCGATGGAAGGGAAATTAGTGCCGCAAAATCCCAATGACGA
ACCTGCAAGTGAATTATTAAAGAGAATTAAAGCTGAAAAAGAAAAACTTATCAGTGAAGGAAAAATCAAACGAGATAAAA
AGGAAACTGAGATATTTCGTGGTGATGATGGGAAACATTATGGGAAGTTTGCTGATGGAAGCACTCAAGAAATTGATGTT
CCTTATGATATTCCTGATACTTGGGAGTGGGTGAGGATAAAATCAATTTATTGGAATTTTGGGCAAAATAAGCCAGAGAA
ATCCTTTAGGTATATAGATACGTCTAGTATTGATAGAAAAAAGAACATAATCAACTACAAAAATCTACAATATCTTTCAC
CTGAACAAGCGCCTTCCCGTGCTAGAAAATTAGTTTCGCAGAATAGTGTCTTATTTTCAACAGTTAGACCATATCTAAAA
AATATTGCTGTAGTTAGAGAACTTAAAGAGTATTTGATAGCTAGTACAGCATTTATTGTTTTGGATACTTTACTTAACGA
AACATATTTGAAATATTATTTATTATCAGATAATTTTATCAATCGTGTTAATAATAAATCTACTGGAACAAGTTATCCTG
CAATCAATGATTATAATTTTAATCTATTATTAATTGCTCTCCCCCCACTATCCGAACAACAACGAATAGTAGAAGCAATC
GAATCAGCTTTAGAAAAAGTAGATGAATATGCTGAAAGTTATAATAGACTAGAACAGCTAGATAAAGAATTTCCAGATAA
ACTAAAAAAATCTATTCTTCAATATGCTATGCAAGGAAAATTAGTTGAACAAGACCCAAATGATGAATCAGTCGAAGTTT
TACTTGAAAAAATACGAGCAGAAAAACAAAAACTCTTTGAAGAAGGCAAGATTAAAAAGAAAGATTTGGACATTTCTATT
GTTTCCCAAGGAGATGATAACTCTTATTATGGGAATAAAGACGAAACAACCTCTTATCCTATATATGAAATCCCAGAAGC
ATGGAGATATATTAAATTTGCTAGCCTAGTTAACTTCAGAATAGGAAAAACTCCTCCACGCAGTGAAGCTACTTTTTGGG
GAACTGAAATACCTTGGGTATCTATATCAGATATGCCTATATCTGGTTATGTAACTAATACGAGAGAATCTATTTCTAAA
TTAGCATTAAAATCAAAGAAAATAGACATTTCACCTAAAGGAACTTTATTAATGAGTTTTAAATTATCTATTGGAAAAGT
TGCCATATTGGATATTCCTGCCACTCATAATGAAGCCATTATATCGATATTCCCCTATGCTAATAAAGAAAATATTATCA
GAGATTACTTAATGATATTTTTGCCACTTATCTCTACTTTAGGTGATTCAAAAGATGCTATCAAAGGGAAAACATTAAAT
AGTACCAGTATCTCCGAATTATTAATCCCTATTTCTAATCATGAAGAGATGAAAAGAATCATTTCCAAAGTTGATTTACT
TTTTCAAAAAGTTTCTCAACTTTTTGAGTAA

Upstream 100 bases:

>100_bases
CCTTTGAGTTGATTCAGAATTATCAAGCGGAAAGAGCAACTTTAAATCATAAGATTGATAATGTATTAGCTGATATTTTG
CAGTTGTTGGAGGACAAATA

Downstream 100 bases:

>100_bases
TAAGTTCCTGTTCCTCAAAAGGAGCTAACGGAATTAATAGCTCGCTTAGTGTAGTTTTAGGAATATTATATAAAGCTTGA
CCTGATAGTTTAGTTATTGC

Product: type I restriction enzyme

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDV
PYDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
NIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAI
ESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISI
VSQGDDNSYYGNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISK
LALKSKKIDISPKGTLLMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLN
STSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE

Sequences:

>Translated_516_residues
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDV
PYDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
NIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAI
ESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISI
VSQGDDNSYYGNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISK
LALKSKKIDISPKGTLLMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLN
STSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE
>Mature_515_residues
TPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVP
YDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKN
IAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAIE
SALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIV
SQGDDNSYYGNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKL
ALKSKKIDISPKGTLLMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNS
TSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 59235; Mature: 59103

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFR
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEE
GDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTSSIDRK
CCCCCCCCCCCCCCCCEECCCCCCCCCCHHEEEEEHHEECCCCCCCCCEEEECCCCCHHH
KNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLIASTAFIV
HHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAI
HHHHHHHHHHEEEEECCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
ESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
EKQKLFEEGKIKKKDLDISIVSQGDDNSYYGNKDETTSYPIYEIPEAWRYIKFASLVNFR
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHH
IGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSF
CCCCCCCCCCCEECCCCCEEEECCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCEEEEE
KLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLN
EECCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
STSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE
CCCHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLISEGKIKRDKKETEIFR
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHEE
GDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTSSIDRK
CCCCCCCCCCCCCCCCEECCCCCCCCCCHHEEEEEHHEECCCCCCCCCEEEECCCCCHHH
KNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLIASTAFIV
HHCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAI
HHHHHHHHHHEEEEECCHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHH
ESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
EKQKLFEEGKIKKKDLDISIVSQGDDNSYYGNKDETTSYPIYEIPEAWRYIKFASLVNFR
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHH
IGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSF
CCCCCCCCCCCEECCCCCEEEECCCCCCCEECCHHHHHHHHHHHHCCCCCCCCCCEEEEE
KLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLN
EECCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
STSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE
CCCHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]