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The map label for this gene is manZ [H]

Identifier: 225858131

GI number: 225858131

Start: 300293

End: 301204

Strand: Reverse

Name: manZ [H]

Synonym: SP70585_0341

Alternate gene names: 225858131

Gene position: 301204-300293 (Counterclockwise)

Preceding gene: 225858132

Following gene: 225858128

Centisome position: 13.79

GC content: 41.01

Gene sequence:

>912_bases
ATGACTGAAAAACTTCAATTAACTAAATCAGATCGTAAAAAAGTTTGGTGGCGTTCAACCTTCTTACAAGGGTCTTGGAA
CTTTGAACGGATGCAAAACTTGGGCTGGGCTTATACACTCATTCCAGCTATCAAAAAACTCTATACTAAAAAAGAAGATC
AAATCGCTGCTCTTGAACGTCACCTTGAGTTCTTCAACACTCATCCATACGTAGCTGCTCCAGTCATGGGGGTTACTCTT
GCGCTTGAAGAAGAACGTGCTAACGGTGTGGAAATCGATGACGCTGCTATCCAAGGGGTTAAAATCGGTATGATGGGACC
TCTTGCTGGTATCGGTGACCCAGTATTCTGGTTTACAGTACGCCCAATCCTTGGATCTCTCGGTGCTTCACTTGCCCTTA
CTGGCAATATCTTGGGGCCACTCCTCTTCTTTGTTGCATGGAACTTGATTCGTATGTCATTCTTGTGGTATGTTCAAGAG
ATTGGATACAAGGCTGGATCAGAAATCACTAAAGATATGTCTGGTGGTATCCTTCAAGATATCACTAAAGGAGCTTCTAT
CCTTGGGATGTTCATTCTTGCTGTCCTTGTTCAACGCTGGGTAAATATTAAATTTGCTTTCGATGTTTCTAAAGTTCAAC
TAGATGAAAAGGCTTATATCCATTGGGATAAATTGCCAGAAGGGTCTAAAGGTATCCAAGAAGCATTCGCACAAGTAGGA
CAAGGATTGTCTCAAACTCCTGAAAAAGTTACTACTTTCCAACAAAACTTGGATATGTTGATTCCTGGATTATCAGGACT
ACTCCTTACTTTACTTTGCATGTACTTACTTAAGAAAAAAGTATCTCCAATCACTATTATCCTTGCCCTCTTCGCAGTGG
GTATTGTGGCACATGTTCTTCACATCATGTAA

Upstream 100 bases:

>100_bases
CTAAAACTGGTGGAAATGGTGGCGGAGGAGCCGCAACTTCTAACGACCCAATCGGCGATATCCTAGAAGACTACTAAGAT
AAGAAAGGACTGAAAACATC

Downstream 100 bases:

>100_bases
TCAAGCAACTAAAAAGGAACCAGGTTCTAAAATCTGATTCCTTTTTTCTATGCTTTTATTCAGCCAAGGCTCCCATTGGA
TCCCATGGTGCAAGTACGAT

Product: PTS system, mannose/fructose/sorbose family, IID component

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAIKKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTL
ALEEERANGVEIDDAAIQGVKIGMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKAYIHWDKLPEGSKGIQEAFAQVG
QGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYLLKKKVSPITIILALFAVGIVAHVLHIM

Sequences:

>Translated_303_residues
MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAIKKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTL
ALEEERANGVEIDDAAIQGVKIGMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKAYIHWDKLPEGSKGIQEAFAQVG
QGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYLLKKKVSPITIILALFAVGIVAHVLHIM
>Mature_302_residues
TEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAIKKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLA
LEEERANGVEIDDAAIQGVKIGMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQEI
GYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKAYIHWDKLPEGSKGIQEAFAQVGQ
GLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYLLKKKVSPITIILALFAVGIVAHVLHIM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=303, Percent_Identity=48.8448844884488, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1789529, Length=303, Percent_Identity=30.3630363036304, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 33786; Mature: 33655

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAIKKLYTKKEDQIAALER
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHH
HLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKIGMMGPLAGIGDPVFWFTV
HHHHHCCCCCHHHHHHHHHEEEEHHCCCCCCCCHHHHCCEEEECCCCCCCCCCCHHHHHH
RPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQEIGYKAGSEITKDMSGGILQD
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHH
ITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKAYIHWDKLPEGSKGIQEAFAQVG
HHHHHHHHHHHHHHHHHHHHHCEEEEEEHHHHEECCHHEEEECCCCCCCHHHHHHHHHHC
QGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYLLKKKVSPITIILALFAVGIVAHVL
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HIM
HHC
>Mature Secondary Structure 
TEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAIKKLYTKKEDQIAALER
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHH
HLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKIGMMGPLAGIGDPVFWFTV
HHHHHCCCCCHHHHHHHHHEEEEHHCCCCCCCCHHHHCCEEEECCCCCCCCCCCHHHHHH
RPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQEIGYKAGSEITKDMSGGILQD
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHH
ITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKAYIHWDKLPEGSKGIQEAFAQVG
HHHHHHHHHHHHHHHHHHHHHCEEEEEEHHHHEECCHHEEEECCCCCCCHHHHHHHHHHC
QGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYLLKKKVSPITIILALFAVGIVAHVL
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HIM
HHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]