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The map label for this gene is narI [H]

Identifier: 225686141

GI number: 225686141

Start: 276297

End: 277022

Strand: Reverse

Name: narI [H]

Synonym: BMEA_B0297

Alternate gene names: 225686141

Gene position: 277022-276297 (Counterclockwise)

Preceding gene: 225686142

Following gene: 225686140

Centisome position: 23.37

GC content: 60.74

Gene sequence:

>726_bases
ATGGCCAGCTATTTCAACACGTTTCTGTTCGGCATCTATCCCTATATCGCGCTGAGCGTCCTCGCACTCGGCTCGATCGT
CCGCTACGACCGCGAACCCTATACCTGGCGCTCCGGCTCCAGCCAGCTTCTGCGCCGCAGGCAGCTCATGTGGGGCTCGG
TCCTGTTCCATCTCGGCGTTCTCGTCATTTTCGCAGGCCACTTTGTGGGCCTTCTGACCCCGATCTGGATCTTCGACACG
CTCGGCATCAGCCATGAGGCAAAGCAGATCCTCGCCATCGTCGCGGGCGGCATTGCCGGCCTGATGGCAATCATCGGCGC
CAGCATGCTGGTGCATCGCCGCCTGTTCGACCCGCGCGTGCGCGCTTCCTCGCGCCCAAGCGACACACTCATCATCATCT
TGTTGTGGGTGCAGCTTCTGCTCGGCCTGAGTTCGATCCCGGTTTCGCTCCAGCACATCGATGGCGGCGAAATGGTCAAG
TTCATGAACTGGGCGCAGGGCATCTTCACCTTCAACCCGGCCGCCTCCAGCTATGTTGCCGATGCTTCCATCGTGTTCAA
GATGCACATCTTCCTCGGGCTCACGATCTTCATCCTGTTCCCGTTCACCCGTCTTGTGCATATGCTGAGTGCGCCGGTGC
GTTATATCTGGCGTCCGGGCTATCAGGTGGTGCGTGAGCGCAACCACACCGCCGCGCCTTCGCATCTGCGCGCTCCTGCG
GAGTAA

Upstream 100 bases:

>100_bases
GCAGGCGAAAATGCCTGCGGGCCGGATCGGCTGCGCATGCAGATGCGCGCCGCACACCGCAAGCCCGCTTCGCCATCAAC
ACAAATCTAGGAGACGGAAG

Downstream 100 bases:

>100_bases
GGTTCATGGTCACACTTTTCGACCGGAAGAATGCAGGTGGACAACCGGCGGGGGATAACCCCGCCGGACATCACGGCCAC
CAGCACCAGTCCGGCTACAC

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MASYFNTFLFGIYPYIALSVLALGSIVRYDREPYTWRSGSSQLLRRRQLMWGSVLFHLGVLVIFAGHFVGLLTPIWIFDT
LGISHEAKQILAIVAGGIAGLMAIIGASMLVHRRLFDPRVRASSRPSDTLIIILLWVQLLLGLSSIPVSLQHIDGGEMVK
FMNWAQGIFTFNPAASSYVADASIVFKMHIFLGLTIFILFPFTRLVHMLSAPVRYIWRPGYQVVRERNHTAAPSHLRAPA
E

Sequences:

>Translated_241_residues
MASYFNTFLFGIYPYIALSVLALGSIVRYDREPYTWRSGSSQLLRRRQLMWGSVLFHLGVLVIFAGHFVGLLTPIWIFDT
LGISHEAKQILAIVAGGIAGLMAIIGASMLVHRRLFDPRVRASSRPSDTLIIILLWVQLLLGLSSIPVSLQHIDGGEMVK
FMNWAQGIFTFNPAASSYVADASIVFKMHIFLGLTIFILFPFTRLVHMLSAPVRYIWRPGYQVVRERNHTAAPSHLRAPA
E
>Mature_240_residues
ASYFNTFLFGIYPYIALSVLALGSIVRYDREPYTWRSGSSQLLRRRQLMWGSVLFHLGVLVIFAGHFVGLLTPIWIFDTL
GISHEAKQILAIVAGGIAGLMAIIGASMLVHRRLFDPRVRASSRPSDTLIIILLWVQLLLGLSSIPVSLQHIDGGEMVKF
MNWAQGIFTFNPAASSYVADASIVFKMHIFLGLTIFILFPFTRLVHMLSAPVRYIWRPGYQVVRERNHTAAPSHLRAPAE

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=226, Percent_Identity=48.2300884955752, Blast_Score=224, Evalue=4e-60,
Organism=Escherichia coli, GI1787738, Length=228, Percent_Identity=49.1228070175439, Blast_Score=215, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 27040; Mature: 26909

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASYFNTFLFGIYPYIALSVLALGSIVRYDREPYTWRSGSSQLLRRRQLMWGSVLFHLGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LVIFAGHFVGLLTPIWIFDTLGISHEAKQILAIVAGGIAGLMAIIGASMLVHRRLFDPRV
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RASSRPSDTLIIILLWVQLLLGLSSIPVSLQHIDGGEMVKFMNWAQGIFTFNPAASSYVA
CCCCCCCCHHHHHHHHHHHHHCHHCCCEEEEECCCHHHHHHHHHHHHHEEECCCHHHHHH
DASIVFKMHIFLGLTIFILFPFTRLVHMLSAPVRYIWRPGYQVVRERNHTAAPSHLRAPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCC
E
C
>Mature Secondary Structure 
ASYFNTFLFGIYPYIALSVLALGSIVRYDREPYTWRSGSSQLLRRRQLMWGSVLFHLGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LVIFAGHFVGLLTPIWIFDTLGISHEAKQILAIVAGGIAGLMAIIGASMLVHRRLFDPRV
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RASSRPSDTLIIILLWVQLLLGLSSIPVSLQHIDGGEMVKFMNWAQGIFTFNPAASSYVA
CCCCCCCCHHHHHHHHHHHHHCHHCCCEEEEECCCHHHHHHHHHHHHHEEECCCHHHHHH
DASIVFKMHIFLGLTIFILFPFTRLVHMLSAPVRYIWRPGYQVVRERNHTAAPSHLRAPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]