| Definition | Brucella melitensis ATCC 23457 chromosome chromosome II, complete sequence. |
|---|---|
| Accession | NC_012442 |
| Length | 1,185,518 |
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The map label for this gene is yqhE [C]
Identifier: 225686038
GI number: 225686038
Start: 167444
End: 168271
Strand: Reverse
Name: yqhE [C]
Synonym: BMEA_B0183
Alternate gene names: 225686038
Gene position: 168271-167444 (Counterclockwise)
Preceding gene: 225686039
Following gene: 225686034
Centisome position: 14.19
GC content: 55.31
Gene sequence:
>828_bases ATGACCGTACCGACGGTTAAACTGAACGACGGCAATCATATTCCGCAGCTCGGTTACGGTGTCTGGCAGATTAGCAATGA TGAAGCGGTGAGCGCAGTCAGCGAGGCGCTGAAAGCCGGTTATCGACACATCGACACCGCCACCATTTACGGCAATGAAG AAGGCGTCGGCAAGGCGATCAACGGTTCGGGCATCGCGCGCGCAGACATCTTCCTGACCACCAAGCTCTGGAACAGCGAT CAGGGCTATGAATCCACGCTCAAGGCTTTCGACACCAGCCTGAAGAAGCTGGGCACCGACTATGTGGATCTCTATCTGAT CCACTGGCCCATGCCATCGAAAGACCTGTTCATGGAAACATGGCGCGCCTTCATCAAACTGAAGGAAGAAGGCCGCGTCA AATCCATCGGTGTATCGAATTTCCGCACTGCCGATCTTGAGCGCCTCATCAAGGAAAGCGGCGTTACGCCGGTCCTGAAC CAGATCGAGTTGCACCCCCAGTTCCAGCAGGATGAACTGCGCCTGTTCCATGGCAAGCACGACATCGCAACGGAAGCCTG GAGCCCGCTCGGCCAGGGCAAACTTCTGGAAGACCCGACGCTGAAATCGATTGCCGAAAAGCACGCAAAGTCGGTGGCAC AGATCATTTTGCGCTGGCACATCGAGACCGGGAATATCGTGATCCCGAAATCGATCACACCTGCCCGCATCAAGGAAAAT TTCGATATTTTCGACTTCACGCTGAACGGCACCGACCACGATGCCATCACCAAGCTTGACATGGCGGATGGCCGCATCGG GCCGAACCCGAGTGTTTTTTCAGCATAA
Upstream 100 bases:
>100_bases CAAAGTCCGAAGAATATTCGTCAAAGAGCTGCCGCGAGGGATAATGTGGTGGCCAGCGCCCCGGAAAACGGCGCAAAAGA TAAGTGGGAAAGGATATATC
Downstream 100 bases:
>100_bases ATGGAGCATGTCCCCAAAAGCGGAAGCCGGTTTTGGGGATTAGAGCGCATCCCGAAAAGTGTGAAGCGGTTTTCGGAAAA GATGCGCGTCAAAACAAAGA
Product: 2,5-diketo-D-gluconic acid reductase A
Products: 2-keto-L-gulonate; NADP [C]
Alternate protein names: NA
Number of amino acids: Translated: 275; Mature: 274
Protein sequence:
>275_residues MTVPTVKLNDGNHIPQLGYGVWQISNDEAVSAVSEALKAGYRHIDTATIYGNEEGVGKAINGSGIARADIFLTTKLWNSD QGYESTLKAFDTSLKKLGTDYVDLYLIHWPMPSKDLFMETWRAFIKLKEEGRVKSIGVSNFRTADLERLIKESGVTPVLN QIELHPQFQQDELRLFHGKHDIATEAWSPLGQGKLLEDPTLKSIAEKHAKSVAQIILRWHIETGNIVIPKSITPARIKEN FDIFDFTLNGTDHDAITKLDMADGRIGPNPSVFSA
Sequences:
>Translated_275_residues MTVPTVKLNDGNHIPQLGYGVWQISNDEAVSAVSEALKAGYRHIDTATIYGNEEGVGKAINGSGIARADIFLTTKLWNSD QGYESTLKAFDTSLKKLGTDYVDLYLIHWPMPSKDLFMETWRAFIKLKEEGRVKSIGVSNFRTADLERLIKESGVTPVLN QIELHPQFQQDELRLFHGKHDIATEAWSPLGQGKLLEDPTLKSIAEKHAKSVAQIILRWHIETGNIVIPKSITPARIKEN FDIFDFTLNGTDHDAITKLDMADGRIGPNPSVFSA >Mature_274_residues TVPTVKLNDGNHIPQLGYGVWQISNDEAVSAVSEALKAGYRHIDTATIYGNEEGVGKAINGSGIARADIFLTTKLWNSDQ GYESTLKAFDTSLKKLGTDYVDLYLIHWPMPSKDLFMETWRAFIKLKEEGRVKSIGVSNFRTADLERLIKESGVTPVLNQ IELHPQFQQDELRLFHGKHDIATEAWSPLGQGKLLEDPTLKSIAEKHAKSVAQIILRWHIETGNIVIPKSITPARIKENF DIFDFTLNGTDHDAITKLDMADGRIGPNPSVFSA
Specific function: Reduction [C]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=39.1156462585034, Blast_Score=197, Evalue=8e-51, Organism=Homo sapiens, GI5453543, Length=294, Percent_Identity=39.1156462585034, Blast_Score=196, Evalue=3e-50, Organism=Homo sapiens, GI223468663, Length=289, Percent_Identity=38.4083044982699, Blast_Score=195, Evalue=3e-50, Organism=Homo sapiens, GI310109922, Length=276, Percent_Identity=39.8550724637681, Blast_Score=195, Evalue=5e-50, Organism=Homo sapiens, GI310109920, Length=294, Percent_Identity=39.1156462585034, Blast_Score=194, Evalue=6e-50, Organism=Homo sapiens, GI45446745, Length=294, Percent_Identity=39.1156462585034, Blast_Score=194, Evalue=6e-50, Organism=Homo sapiens, GI4503285, Length=294, Percent_Identity=39.1156462585034, Blast_Score=194, Evalue=6e-50, Organism=Homo sapiens, GI24497577, Length=293, Percent_Identity=34.4709897610922, Blast_Score=191, Evalue=5e-49, Organism=Homo sapiens, GI5174391, Length=293, Percent_Identity=34.4709897610922, Blast_Score=191, Evalue=5e-49, Organism=Homo sapiens, GI93277124, Length=294, Percent_Identity=38.4353741496599, Blast_Score=191, Evalue=8e-49, Organism=Homo sapiens, GI24497585, Length=294, Percent_Identity=38.0952380952381, Blast_Score=189, Evalue=3e-48, Organism=Homo sapiens, GI5174695, Length=294, Percent_Identity=37.7551020408163, Blast_Score=184, Evalue=9e-47, Organism=Homo sapiens, GI291291012, Length=263, Percent_Identity=38.0228136882129, Blast_Score=176, Evalue=2e-44, Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=37.5451263537906, Blast_Score=174, Evalue=1e-43, Organism=Homo sapiens, GI4502049, Length=279, Percent_Identity=38.3512544802867, Blast_Score=171, Evalue=9e-43, Organism=Homo sapiens, GI300116271, Length=277, Percent_Identity=33.9350180505415, Blast_Score=144, Evalue=9e-35, Organism=Homo sapiens, GI310109926, Length=126, Percent_Identity=44.4444444444444, Blast_Score=105, Evalue=4e-23, Organism=Homo sapiens, GI207028673, Length=126, Percent_Identity=44.4444444444444, Blast_Score=105, Evalue=5e-23, Organism=Homo sapiens, GI310109924, Length=148, Percent_Identity=38.5135135135135, Blast_Score=102, Evalue=5e-22, Organism=Escherichia coli, GI87082198, Length=278, Percent_Identity=51.4388489208633, Blast_Score=300, Evalue=6e-83, Organism=Escherichia coli, GI1786400, Length=257, Percent_Identity=37.3540856031128, Blast_Score=179, Evalue=2e-46, Organism=Escherichia coli, GI1788081, Length=275, Percent_Identity=30.1818181818182, Blast_Score=118, Evalue=5e-28, Organism=Escherichia coli, GI48994888, Length=237, Percent_Identity=28.6919831223629, Blast_Score=76, Evalue=2e-15, Organism=Escherichia coli, GI1788070, Length=315, Percent_Identity=22.2222222222222, Blast_Score=63, Evalue=2e-11, Organism=Caenorhabditis elegans, GI71998625, Length=270, Percent_Identity=38.8888888888889, Blast_Score=194, Evalue=3e-50, Organism=Caenorhabditis elegans, GI17561298, Length=270, Percent_Identity=35.5555555555556, Blast_Score=184, Evalue=5e-47, Organism=Caenorhabditis elegans, GI17537077, Length=291, Percent_Identity=36.7697594501718, Blast_Score=183, Evalue=1e-46, Organism=Caenorhabditis elegans, GI17537075, Length=301, Percent_Identity=35.5481727574751, Blast_Score=182, Evalue=1e-46, Organism=Caenorhabditis elegans, GI17564128, Length=298, Percent_Identity=35.9060402684564, Blast_Score=181, Evalue=5e-46, Organism=Caenorhabditis elegans, GI17538386, Length=272, Percent_Identity=37.1323529411765, Blast_Score=176, Evalue=9e-45, Organism=Caenorhabditis elegans, GI17566692, Length=283, Percent_Identity=36.7491166077738, Blast_Score=174, Evalue=4e-44, Organism=Caenorhabditis elegans, GI17552492, Length=265, Percent_Identity=34.7169811320755, Blast_Score=174, Evalue=5e-44, Organism=Caenorhabditis elegans, GI17550248, Length=288, Percent_Identity=37.1527777777778, Blast_Score=172, Evalue=1e-43, Organism=Caenorhabditis elegans, GI17537079, Length=300, Percent_Identity=33, Blast_Score=162, Evalue=2e-40, Organism=Caenorhabditis elegans, GI17561300, Length=254, Percent_Identity=34.251968503937, Blast_Score=144, Evalue=5e-35, Organism=Caenorhabditis elegans, GI17562292, Length=254, Percent_Identity=33.0708661417323, Blast_Score=141, Evalue=4e-34, Organism=Saccharomyces cerevisiae, GI6324694, Length=301, Percent_Identity=42.1926910299003, Blast_Score=218, Evalue=8e-58, Organism=Saccharomyces cerevisiae, GI6320576, Length=290, Percent_Identity=41.3793103448276, Blast_Score=212, Evalue=6e-56, Organism=Saccharomyces cerevisiae, GI6322556, Length=258, Percent_Identity=35.6589147286822, Blast_Score=168, Evalue=7e-43, Organism=Saccharomyces cerevisiae, GI6319625, Length=279, Percent_Identity=38.3512544802867, Blast_Score=167, Evalue=2e-42, Organism=Saccharomyces cerevisiae, GI6320079, Length=280, Percent_Identity=39.2857142857143, Blast_Score=163, Evalue=3e-41, Organism=Saccharomyces cerevisiae, GI6321896, Length=303, Percent_Identity=33.3333333333333, Blast_Score=153, Evalue=3e-38, Organism=Drosophila melanogaster, GI24657054, Length=278, Percent_Identity=41.3669064748201, Blast_Score=203, Evalue=9e-53, Organism=Drosophila melanogaster, GI21356425, Length=282, Percent_Identity=40.0709219858156, Blast_Score=195, Evalue=3e-50, Organism=Drosophila melanogaster, GI24662789, Length=301, Percent_Identity=36.5448504983389, Blast_Score=194, Evalue=4e-50, Organism=Drosophila melanogaster, GI24663317, Length=280, Percent_Identity=39.2857142857143, Blast_Score=193, Evalue=1e-49, Organism=Drosophila melanogaster, GI20129731, Length=275, Percent_Identity=37.8181818181818, Blast_Score=192, Evalue=3e-49, Organism=Drosophila melanogaster, GI24644950, Length=296, Percent_Identity=37.8378378378378, Blast_Score=187, Evalue=5e-48, Organism=Drosophila melanogaster, GI45553081, Length=275, Percent_Identity=38.9090909090909, Blast_Score=182, Evalue=3e-46, Organism=Drosophila melanogaster, GI221378297, Length=316, Percent_Identity=35.7594936708861, Blast_Score=181, Evalue=6e-46, Organism=Drosophila melanogaster, GI24662781, Length=300, Percent_Identity=39.6666666666667, Blast_Score=180, Evalue=9e-46, Organism=Drosophila melanogaster, GI24662785, Length=297, Percent_Identity=39.3939393939394, Blast_Score=172, Evalue=2e-43, Organism=Drosophila melanogaster, GI281366140, Length=239, Percent_Identity=38.4937238493724, Blast_Score=151, Evalue=6e-37,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: NA
Molecular weight: Translated: 30641; Mature: 30510
Theoretical pI: Translated: 6.24; Mature: 6.24
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 1.5 %Met (Translated Protein) 1.5 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 1.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTVPTVKLNDGNHIPQLGYGVWQISNDEAVSAVSEALKAGYRHIDTATIYGNEEGVGKAI CCCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEE NGSGIARADIFLTTKLWNSDQGYESTLKAFDTSLKKLGTDYVDLYLIHWPMPSKDLFMET CCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHH WRAFIKLKEEGRVKSIGVSNFRTADLERLIKESGVTPVLNQIELHPQFQQDELRLFHGKH HHHHHEECCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHEECCCCCCCCHHHHHCCCC DIATEAWSPLGQGKLLEDPTLKSIAEKHAKSVAQIILRWHIETGNIVIPKSITPARIKEN HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCHHHHHCC FDIFDFTLNGTDHDAITKLDMADGRIGPNPSVFSA CCEEEEEECCCCCHHEEEEECCCCCCCCCCCCCCC >Mature Secondary Structure TVPTVKLNDGNHIPQLGYGVWQISNDEAVSAVSEALKAGYRHIDTATIYGNEEGVGKAI CCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEE NGSGIARADIFLTTKLWNSDQGYESTLKAFDTSLKKLGTDYVDLYLIHWPMPSKDLFMET CCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHH WRAFIKLKEEGRVKSIGVSNFRTADLERLIKESGVTPVLNQIELHPQFQQDELRLFHGKH HHHHHEECCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHEECCCCCCCCHHHHHCCCC DIATEAWSPLGQGKLLEDPTLKSIAEKHAKSVAQIILRWHIETGNIVIPKSITPARIKEN HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEECCCCCHHHHHCC FDIFDFTLNGTDHDAITKLDMADGRIGPNPSVFSA CCEEEEEECCCCCHHEEEEECCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: 2,5-diketo-D-gluconate; NADPH [C]
Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA