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The map label for this gene is eda [H]

Identifier: 225685950

GI number: 225685950

Start: 85845

End: 86483

Strand: Reverse

Name: eda [H]

Synonym: BMEA_B0088

Alternate gene names: 225685950

Gene position: 86483-85845 (Counterclockwise)

Preceding gene: 225685951

Following gene: 225685947

Centisome position: 7.29

GC content: 61.5

Gene sequence:

>639_bases
ATGTCGCAGAAAACCGATCTTCTTCTTCCCATCATGAAAGGCCAGCCGGTCATTCCGGTTCTGCTGATCGACAAGGTGGA
ACATGCCGTTCCACTGGCGCGCGCGCTTGCCAGAGGCGGGTTGCCGGCAATCGAGATCACGCTGCGCACTGCGGCGGCGC
TGGACGCCATCCGTGCGGTTGCGAATGAAGTGCCGGAAGCCATCGTCGGCGCAGGTACGATCCTCAATGCGAAGCAATAT
GAAGACGCTGCCAAGGCCGGTTCCCGCTTCATCGTCAGCCCCGGCGCGACGAAATATATCGTCGCCGCTGCAAACGACAG
CGACGTTCCCCTTCTTCCTGCCGCCATCACGCCCGGCGAAATGCTGGCGCTGCGCGAGGAAGGCTATACGCATCTGAAAT
TCTTCCCCGCCGAACAGGCTGGCGGCGCGCCTTTCCTGAAAGCCCTGTCATCACCGCTTGCTGGCACGTTCTTCTGCCCG
ACAGGCGGCATCAGCCTTGCCAATGCCAGAACCTATCTGCCCCTGCCGAACGTGCTCTGCGTCGGCGGCTCGTGGGTTGC
GCCCAAGGAACTGGTGGAAGCAGGCAACTGGAACGCCATCACCGATCTTGCAAAGGATGCAGCCGCGCTGAAAGCCTGA

Upstream 100 bases:

>100_bases
GATGAAGCGCCCCTATGCGGACTGCATCGGCGCTTCATCCATTTGTTCTGAAGCCTTGCGTATTTCATCAAGGGGATGCG
CCCAACCGTCGAGCGAGCCG

Downstream 100 bases:

>100_bases
TCTCACCGCACTGCAAGAGCGCAAAAGGGGCCGCAACGGCCCCTTTTCCTTGCATGATCTGGTGGTCAATTGGTTTCGGT
GAACTTCGCCACGGTCAGGA

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSQKTDLLLPIMKGQPVIPVLLIDKVEHAVPLARALARGGLPAIEITLRTAAALDAIRAVANEVPEAIVGAGTILNAKQY
EDAAKAGSRFIVSPGATKYIVAAANDSDVPLLPAAITPGEMLALREEGYTHLKFFPAEQAGGAPFLKALSSPLAGTFFCP
TGGISLANARTYLPLPNVLCVGGSWVAPKELVEAGNWNAITDLAKDAAALKA

Sequences:

>Translated_212_residues
MSQKTDLLLPIMKGQPVIPVLLIDKVEHAVPLARALARGGLPAIEITLRTAAALDAIRAVANEVPEAIVGAGTILNAKQY
EDAAKAGSRFIVSPGATKYIVAAANDSDVPLLPAAITPGEMLALREEGYTHLKFFPAEQAGGAPFLKALSSPLAGTFFCP
TGGISLANARTYLPLPNVLCVGGSWVAPKELVEAGNWNAITDLAKDAAALKA
>Mature_211_residues
SQKTDLLLPIMKGQPVIPVLLIDKVEHAVPLARALARGGLPAIEITLRTAAALDAIRAVANEVPEAIVGAGTILNAKQYE
DAAKAGSRFIVSPGATKYIVAAANDSDVPLLPAAITPGEMLALREEGYTHLKFFPAEQAGGAPFLKALSSPLAGTFFCPT
GGISLANARTYLPLPNVLCVGGSWVAPKELVEAGNWNAITDLAKDAAALKA

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=182, Percent_Identity=50.5494505494505, Blast_Score=191, Evalue=2e-50,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 21959; Mature: 21828

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKTDLLLPIMKGQPVIPVLLIDKVEHAVPLARALARGGLPAIEITLRTAAALDAIRAV
CCCCCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
ANEVPEAIVGAGTILNAKQYEDAAKAGSRFIVSPGATKYIVAAANDSDVPLLPAAITPGE
HHHHHHHHHCCCCEECHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCEEEECCCCCH
MLALREEGYTHLKFFPAEQAGGAPFLKALSSPLAGTFFCPTGGISLANARTYLPLPNVLC
HEEECCCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEECCCCCEEECCCEEECCCCCEEE
VGGSWVAPKELVEAGNWNAITDLAKDAAALKA
ECCCCCCHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQKTDLLLPIMKGQPVIPVLLIDKVEHAVPLARALARGGLPAIEITLRTAAALDAIRAV
CCCCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
ANEVPEAIVGAGTILNAKQYEDAAKAGSRFIVSPGATKYIVAAANDSDVPLLPAAITPGE
HHHHHHHHHCCCCEECHHHHHHHHHCCCEEEECCCCCEEEEEECCCCCCCEEEECCCCCH
MLALREEGYTHLKFFPAEQAGGAPFLKALSSPLAGTFFCPTGGISLANARTYLPLPNVLC
HEEECCCCCEEEEEECCCCCCCCHHHHHHCCCCCCEEECCCCCEEECCCEEECCCCCEEE
VGGSWVAPKELVEAGNWNAITDLAKDAAALKA
ECCCCCCHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]