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The map label for this gene is purF [H]

Identifier: 225851982

GI number: 225851982

Start: 466554

End: 468011

Strand: Reverse

Name: purF [H]

Synonym: BMEA_A0480

Alternate gene names: 225851982

Gene position: 468011-466554 (Counterclockwise)

Preceding gene: 225851984

Following gene: 225851981

Centisome position: 22.02

GC content: 59.19

Gene sequence:

>1458_bases
ATGCTGGATGATGACACCCTGCATGAAGAATGTGGTGTATTCGGCATTCTGGGCCATGAGGATGCGGCTGCACTGACCGC
GCTCGGCCTGCATGCGCTCCAGCATCGCGGTCAGGAAGCCGCCGGCATTGTTTCCTATCATAATCGCCGTTTCCATTCCG
AACGCCATATGGGCCTCGTCGGCGACCATTTCACCGATGCGGCAACACTGAACCGCCTGCCGGGCAACCGCGCCATCGGC
CATGTGCGTTATTCCACAACCGGCGAGACGATCCTGCGCAATGTACAGCCGCTGTTTGCGGAACTGGAAGTCGGCGGCAT
CGCCATTGCCCATAACGGCAATTTCACCAATGGCATTACGCTGCGCAAACAGTTGATTGCTTCCGGCGCGATCTTCCAGG
CAACGTCGGATACGGAAGTCGTGCTGCACATGATTGCCCGTTCGCGGCACTCCTCCTCAACCGACCGTTTCGTGGAAGCG
ATCCGCCAGGTTGAAGGCGGCTATGCCATGCTGGCGCTTACCCGCACCAAGCTGATCGCCGCGCGCGATCCCATCGGCAT
TCGCCCGCTCGTCATGGGCGAGCTGGACGGCAAGCCGATCTTCTGCTCGGAAACCTGCGCTCTCGATATTATCGGCGCGA
AATATATTCGCGACGTCGAGAATGGCGAAGTGGTAGTCTGCGAAATCCAGAAGGATGGCTCCATCACCACCCGATCCATC
AAGCCGGAAAATCCGCAGCCGGAGCGGCTGTGCCTTTTTGAATATGTTTATTTCGCACGGCCGGATTCGGTAGTGGGTGG
CCGCAGCGTCTATGTGGCGCGCAAGAACATGGGCATGAACCTTGCCGCCGAGGCGGGCGTGGATGCCGATGTGGTCGTGC
CCGTACCGGATGGCGGTACGCCGGCAGCGCTGGGCTTTGCGCAGGCAAGCGGTATTCCGTTTGAATATGGCATCATCCGC
AACCATTATGTGGGCCGCACCTTCATTGAGCCTACACAGCAGATCCGCGCGCTTGGCGTGAAGCTGAAACATTCAGCCAA
TCGCGCTATGATTGAAGGCAAGCGGGTCGTTCTGGTGGATGATTCCATCGTGCGCGGCACCACCTCGGTCAAGATCGTTC
AGATGATCCGCGACGCAGGCGCAACCGAAGTCCATATGCGCGTGGCAAGCCCGATGATCTTCCACCCGGATTTTTACGGT
ATCGACACCCCCCATGCCGATAAGCTGCTGGCCAACCAGCACGAAAGCCTCGAATCCATGTGCCGCTACATCGGCGCCGA
TTCGCTGGCCTTCCTTTCCATCGACGGGCTCTACAAGGCCGTTGGCGGAAAGCCGCGTGATCCAAAGGCGCCGACCTTCA
CCGATCACTATTTCACGGGCGAGTATCCGACCCGCCTTCTGGATCAGGAAGGCGAAAGCAACGTCCACACCTTGTCGCTG
CTCGCCAGCAACGGCTGA

Upstream 100 bases:

>100_bases
GGAAAACGTGCCTCCGCTTTTCCGCGATATGCTTAAGTTGCCTCTGCAATCGAAAGGCCTTGCGGCAATGACCAGCCATT
CTTCTGAAAATGCCCCCTTT

Downstream 100 bases:

>100_bases
TCGCCCTTAACTACGCAGGCGGCTTTCAGGCCGCCTGTTTCATGTTTCTGAACGCATACGGGGACGCAATGAATATCGAT
CTTACGGGCCGCCTCGCGCT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MLDDDTLHEECGVFGILGHEDAAALTALGLHALQHRGQEAAGIVSYHNRRFHSERHMGLVGDHFTDAATLNRLPGNRAIG
HVRYSTTGETILRNVQPLFAELEVGGIAIAHNGNFTNGITLRKQLIASGAIFQATSDTEVVLHMIARSRHSSSTDRFVEA
IRQVEGGYAMLALTRTKLIAARDPIGIRPLVMGELDGKPIFCSETCALDIIGAKYIRDVENGEVVVCEIQKDGSITTRSI
KPENPQPERLCLFEYVYFARPDSVVGGRSVYVARKNMGMNLAAEAGVDADVVVPVPDGGTPAALGFAQASGIPFEYGIIR
NHYVGRTFIEPTQQIRALGVKLKHSANRAMIEGKRVVLVDDSIVRGTTSVKIVQMIRDAGATEVHMRVASPMIFHPDFYG
IDTPHADKLLANQHESLESMCRYIGADSLAFLSIDGLYKAVGGKPRDPKAPTFTDHYFTGEYPTRLLDQEGESNVHTLSL
LASNG

Sequences:

>Translated_485_residues
MLDDDTLHEECGVFGILGHEDAAALTALGLHALQHRGQEAAGIVSYHNRRFHSERHMGLVGDHFTDAATLNRLPGNRAIG
HVRYSTTGETILRNVQPLFAELEVGGIAIAHNGNFTNGITLRKQLIASGAIFQATSDTEVVLHMIARSRHSSSTDRFVEA
IRQVEGGYAMLALTRTKLIAARDPIGIRPLVMGELDGKPIFCSETCALDIIGAKYIRDVENGEVVVCEIQKDGSITTRSI
KPENPQPERLCLFEYVYFARPDSVVGGRSVYVARKNMGMNLAAEAGVDADVVVPVPDGGTPAALGFAQASGIPFEYGIIR
NHYVGRTFIEPTQQIRALGVKLKHSANRAMIEGKRVVLVDDSIVRGTTSVKIVQMIRDAGATEVHMRVASPMIFHPDFYG
IDTPHADKLLANQHESLESMCRYIGADSLAFLSIDGLYKAVGGKPRDPKAPTFTDHYFTGEYPTRLLDQEGESNVHTLSL
LASNG
>Mature_485_residues
MLDDDTLHEECGVFGILGHEDAAALTALGLHALQHRGQEAAGIVSYHNRRFHSERHMGLVGDHFTDAATLNRLPGNRAIG
HVRYSTTGETILRNVQPLFAELEVGGIAIAHNGNFTNGITLRKQLIASGAIFQATSDTEVVLHMIARSRHSSSTDRFVEA
IRQVEGGYAMLALTRTKLIAARDPIGIRPLVMGELDGKPIFCSETCALDIIGAKYIRDVENGEVVVCEIQKDGSITTRSI
KPENPQPERLCLFEYVYFARPDSVVGGRSVYVARKNMGMNLAAEAGVDADVVVPVPDGGTPAALGFAQASGIPFEYGIIR
NHYVGRTFIEPTQQIRALGVKLKHSANRAMIEGKRVVLVDDSIVRGTTSVKIVQMIRDAGATEVHMRVASPMIFHPDFYG
IDTPHADKLLANQHESLESMCRYIGADSLAFLSIDGLYKAVGGKPRDPKAPTFTDHYFTGEYPTRLLDQEGESNVHTLSL
LASNG

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=470, Percent_Identity=40, Blast_Score=326, Evalue=2e-89,
Organism=Escherichia coli, GI1788651, Length=481, Percent_Identity=41.3721413721414, Blast_Score=308, Evalue=8e-85,
Organism=Escherichia coli, GI1790167, Length=240, Percent_Identity=30, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=484, Percent_Identity=38.0165289256198, Blast_Score=290, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6323958, Length=484, Percent_Identity=36.3636363636364, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24659598, Length=494, Percent_Identity=39.0688259109312, Blast_Score=327, Evalue=8e-90,
Organism=Drosophila melanogaster, GI28573187, Length=501, Percent_Identity=39.3213572854291, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24659604, Length=473, Percent_Identity=39.323467230444, Blast_Score=320, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52794; Mature: 52794

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDDTLHEECGVFGILGHEDAAALTALGLHALQHRGQEAAGIVSYHNRRFHSERHMGLV
CCCCCHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
GDHFTDAATLNRLPGNRAIGHVRYSTTGETILRNVQPLFAELEVGGIAIAHNGNFTNGIT
CCCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHEEEECCEEEEECCCCCCCHH
LRKQLIASGAIFQATSDTEVVLHMIARSRHSSSTDRFVEAIRQVEGGYAMLALTRTKLIA
HHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEHHHEEE
ARDPIGIRPLVMGELDGKPIFCSETCALDIIGAKYIRDVENGEVVVCEIQKDGSITTRSI
ECCCCCCCEEEEECCCCCEEEECCCCEEEEHHHHHHHCCCCCCEEEEEEECCCCEEEECC
KPENPQPERLCLFEYVYFARPDSVVGGRSVYVARKNMGMNLAAEAGVDADVVVPVPDGGT
CCCCCCCCCEEEEEHHHHCCCCCCCCCCEEEEEECCCCCEEEHHCCCCCEEEEECCCCCC
PAALGFAQASGIPFEYGIIRNHYVGRTFIEPTQQIRALGVKLKHSANRAMIEGKRVVLVD
CHHEEHHHHCCCCEEECCHHCCCCCCHHCCHHHHHHHHCEEEECCCCCEEECCCEEEEEC
DSIVRGTTSVKIVQMIRDAGATEVHMRVASPMIFHPDFYGIDTPHADKLLANQHESLESM
CCHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHH
CRYIGADSLAFLSIDGLYKAVGGKPRDPKAPTFTDHYFTGEYPTRLLDQEGESNVHTLSL
HHHHCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCEECCCCCHHHHCCCCCCCEEEEEE
LASNG
EECCC
>Mature Secondary Structure
MLDDDTLHEECGVFGILGHEDAAALTALGLHALQHRGQEAAGIVSYHNRRFHSERHMGLV
CCCCCHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
GDHFTDAATLNRLPGNRAIGHVRYSTTGETILRNVQPLFAELEVGGIAIAHNGNFTNGIT
CCCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHEEEECCEEEEECCCCCCCHH
LRKQLIASGAIFQATSDTEVVLHMIARSRHSSSTDRFVEAIRQVEGGYAMLALTRTKLIA
HHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEHHHEEE
ARDPIGIRPLVMGELDGKPIFCSETCALDIIGAKYIRDVENGEVVVCEIQKDGSITTRSI
ECCCCCCCEEEEECCCCCEEEECCCCEEEEHHHHHHHCCCCCCEEEEEEECCCCEEEECC
KPENPQPERLCLFEYVYFARPDSVVGGRSVYVARKNMGMNLAAEAGVDADVVVPVPDGGT
CCCCCCCCCEEEEEHHHHCCCCCCCCCCEEEEEECCCCCEEEHHCCCCCEEEEECCCCCC
PAALGFAQASGIPFEYGIIRNHYVGRTFIEPTQQIRALGVKLKHSANRAMIEGKRVVLVD
CHHEEHHHHCCCCEEECCHHCCCCCCHHCCHHHHHHHHCEEEECCCCCEEECCCEEEEEC
DSIVRGTTSVKIVQMIRDAGATEVHMRVASPMIFHPDFYGIDTPHADKLLANQHESLESM
CCHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHH
CRYIGADSLAFLSIDGLYKAVGGKPRDPKAPTFTDHYFTGEYPTRLLDQEGESNVHTLSL
HHHHCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCEECCCCCHHHHCCCCCCCEEEEEE
LASNG
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA