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The map label for this gene is sphR [H]

Identifier: 222527373

GI number: 222527373

Start: 5154690

End: 5155382

Strand: Direct

Name: sphR [H]

Synonym: Chy400_4163

Alternate gene names: 222527373

Gene position: 5154690-5155382 (Clockwise)

Preceding gene: 222527372

Following gene: 222527374

Centisome position: 97.83

GC content: 52.81

Gene sequence:

>693_bases
ATGCGTCAGACGATCTTGATTATCGATGATCATCTCAATGTACGCACTATGATTGCCGACTACTTGCAAGAGATTGGATA
TCGCGTCGTTACAGCAAGCGACGGCGCGGAGGGCCTCATTATTGCCCGCCGCGTTCGTCCCGACCTGGTCTTGCTCGATA
TCATGATGCCCAAACTGGATGGCTTTGGTTTCTTACAGGCGTATCGCCGGGAACATCAGGCACCGGTCATCTTATTGACT
GCGCGAATTGATGAAACGGACAAGGTAGTCGGATTGGAACTGGGAGCCGACGATTATGTGACCAAACCCTTCAGCCTGAA
AGAATTGGTGGCACGAATCAGGGCAGTCTTGCGTCGAACAGCCGCAGCACGGCAACCGAATGAAGAACAGCCGTTGCGCA
TTGGCAACCTTGAACTGAATCGTGCAACCCGCATGGTAACGGTTGACGAACGGCCCATATACCTGACACCATCTGAGTTT
GCGTTACTGGCGTTGCTCATGGCTTCACCGGGACGTGTGTACACTCGCGAGCAACTCCTTGAACACCTCCAGGGCAACAG
TTATGAAGGGGTTGAACGAACGATTGACGTACACATTCGCAATCTACGCCGCAAAATCGAGCCGGATCCCACCAATCCAA
CCTACATCGAAACTGTCTTCGGCATCGGGTACCGCTGCCGTCCACTGAGTTAA

Upstream 100 bases:

>100_bases
TGATCCTCACTTACCAGCTACAGGCCAGAGCTGATTTTGTTACAGTATCAGCAGCTTCACGTGTCGATCAAACGCCACGA
ATGAGCGAAAGGAGTCAGTG

Downstream 100 bases:

>100_bases
GCCGGTGGAGGATGTATGCTTCGTTCGCTTACGACTCGCCTCATTCTGGCCTTTGCCCTGACCAGTCTGGTCGGTATTGG
ACTGGCGGCACTCCTGGTTC

Product: winged helix family two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRQTILIIDDHLNVRTMIADYLQEIGYRVVTASDGAEGLIIARRVRPDLVLLDIMMPKLDGFGFLQAYRREHQAPVILLT
ARIDETDKVVGLELGADDYVTKPFSLKELVARIRAVLRRTAAARQPNEEQPLRIGNLELNRATRMVTVDERPIYLTPSEF
ALLALLMASPGRVYTREQLLEHLQGNSYEGVERTIDVHIRNLRRKIEPDPTNPTYIETVFGIGYRCRPLS

Sequences:

>Translated_230_residues
MRQTILIIDDHLNVRTMIADYLQEIGYRVVTASDGAEGLIIARRVRPDLVLLDIMMPKLDGFGFLQAYRREHQAPVILLT
ARIDETDKVVGLELGADDYVTKPFSLKELVARIRAVLRRTAAARQPNEEQPLRIGNLELNRATRMVTVDERPIYLTPSEF
ALLALLMASPGRVYTREQLLEHLQGNSYEGVERTIDVHIRNLRRKIEPDPTNPTYIETVFGIGYRCRPLS
>Mature_230_residues
MRQTILIIDDHLNVRTMIADYLQEIGYRVVTASDGAEGLIIARRVRPDLVLLDIMMPKLDGFGFLQAYRREHQAPVILLT
ARIDETDKVVGLELGADDYVTKPFSLKELVARIRAVLRRTAAARQPNEEQPLRIGNLELNRATRMVTVDERPIYLTPSEF
ALLALLMASPGRVYTREQLLEHLQGNSYEGVERTIDVHIRNLRRKIEPDPTNPTYIETVFGIGYRCRPLS

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=41.0714285714286, Blast_Score=173, Evalue=9e-45,
Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=42.1052631578947, Blast_Score=171, Evalue=3e-44,
Organism=Escherichia coli, GI1789809, Length=224, Percent_Identity=39.7321428571429, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1790860, Length=229, Percent_Identity=37.5545851528384, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=37.6681614349776, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI2367329, Length=224, Percent_Identity=40.625, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI87082012, Length=220, Percent_Identity=39.0909090909091, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=39.3665158371041, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1790863, Length=226, Percent_Identity=34.9557522123894, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787229, Length=228, Percent_Identity=35.9649122807018, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=35.2941176470588, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1790552, Length=177, Percent_Identity=43.5028248587571, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=31.6239316239316, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=29.8642533936652, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788550, Length=106, Percent_Identity=42.4528301886792, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790299, Length=130, Percent_Identity=32.3076923076923, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=37.8640776699029, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787487, Length=113, Percent_Identity=31.858407079646, Blast_Score=65, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322000, Length=160, Percent_Identity=28.75, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26249; Mature: 26249

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQTILIIDDHLNVRTMIADYLQEIGYRVVTASDGAEGLIIARRVRPDLVLLDIMMPKLD
CCCEEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCEEEEEEHHHCCC
GFGFLQAYRREHQAPVILLTARIDETDKVVGLELGADDYVTKPFSLKELVARIRAVLRRT
CCHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
AAARQPNEEQPLRIGNLELNRATRMVTVDERPIYLTPSEFALLALLMASPGRVYTREQLL
HHHCCCCCCCCEEECCEEECCCEEEEEECCCCEEECCHHHHHHHHHHCCCCCCHHHHHHH
EHLQGNSYEGVERTIDVHIRNLRRKIEPDPTNPTYIETVFGIGYRCRPLS
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECC
>Mature Secondary Structure
MRQTILIIDDHLNVRTMIADYLQEIGYRVVTASDGAEGLIIARRVRPDLVLLDIMMPKLD
CCCEEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEEECCCCEEEEEEHHHCCC
GFGFLQAYRREHQAPVILLTARIDETDKVVGLELGADDYVTKPFSLKELVARIRAVLRRT
CCHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
AAARQPNEEQPLRIGNLELNRATRMVTVDERPIYLTPSEFALLALLMASPGRVYTREQLL
HHHCCCCCCCCEEECCEEECCCEEEEEECCCCEEECCHHHHHHHHHHCCCCCCHHHHHHH
EHLQGNSYEGVERTIDVHIRNLRRKIEPDPTNPTYIETVFGIGYRCRPLS
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]