Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 222527315

GI number: 222527315

Start: 5076407

End: 5078575

Strand: Direct

Name: pleD [H]

Synonym: Chy400_4100

Alternate gene names: 222527315

Gene position: 5076407-5078575 (Clockwise)

Preceding gene: 222527314

Following gene: 222527316

Centisome position: 96.35

GC content: 56.15

Gene sequence:

>2169_bases
ATGAAACCCGATCCCGCTGAACAGTTTGAAGCACTGCGCAAACGGTATGCCGCGCAAATTGGTGAACGTGTGGCGGCTAT
TCATGCTGCCTGGAACCAGCTTGTCCAGAGCGACTGGGATAAGACGGCTGGCGTAGAGCTACGCCAGCTTGCGCACAAAC
TGGCCGGTTCAGCAGCGATGTTTGGATTCGCTAACCTCGGTGCTGTAGCGCATGACATCGATCTCAGGCTGAAAGAGTGG
CTTGAACACGACCAGTCCCTGGACACTCTTGCCCGTCAACATCTGGCCCAACAGATTGCATTGCTACAACATGCGGTAGG
CGAATACCGCACTTCCACAGCGGTACCGGAAACGAATCAACCCACACCATCAACAGCGCCATCAGAGCCACCACCAGTAC
CCCCAGCAAACACGGAGCCTGCACCGCCTATCCCGCAACCGCAACCCCCAGCAACGCCACGCGAGCCAAACGGTCGTCCG
CAAACCATTGTTTTGGTCGAAGATGATCGCGAGTTCGCTGCGAAACTGGTCGGTCAGTTGCGCCTCTTCAACTATGATGT
CCATCTGGTGAACGATCTGGAAGGACTGCCAACCCTGATCCGTGCTATCAATCCGGCAGCCATCATTATGGACATCGTCT
TCCCAGAGGGTGATCTGGCCGGTACCGAGACCATTGCCAACCTGCAACGCGAACGCACCGAACCAATACCGGTCATCTAT
ATCTCGCAACGCGACGACTTTACTGCCCGTCTGGCAGCAGTACGCAGTGGCGGTCATGCCTATTTCGCCAAACCGGTCGA
TGCCGGGCAGTTGATCAATGCCCTCGACCGGCTGACCGGTGTCGAAGAAAATACCCCCCTGCGCGTATTGATTGTGGATG
ACGATCAGGAGTTTGGGCACAACTGTGTGCACAGCCTGGAAATAGCCGGCATCCATGCGCGGGCGATTACCGATCCGCGC
GAGACCCTGAACCAGTTGCAGGTATTTCAACCCGATCTCATCGTCCTCGATATGTATATGCCGATTTGCAGTGGTAAAGA
ACTGGCCAGCATCATTCGCCAGCAAGATCGCTACGTCGGCATGCCTATCGTCTTTCTCTCGGCTGAGACCAATCGGTTAC
AGCAACAATCGGTACTCTCATTGGGGGCCGACGACTTTCTTGCCAAACCAATCTCACCTGCCGATCTGGTAGCAGCAATC
TCGGCACGAGCGATGCGCGCACGCTATCTCCAGCGCTTTCTCATTCGTGATAGTCTGACCGGATTGCTGAATCATCAGGC
GGTTGAAGAGGAATTGAGTCACGAGTTCGCCCGTGCCGAGCGCCTGCAACAACCGTTGAGTATCGCCATTATTGATATCG
ATCACTTCAAGCGCGTTAACGACTCGTATGGGCATCTGATCGGCGATCAGGTCTTACGCAGCTTTGCCCGCCTCCTGCAA
CAGCGGCTGCGCAAAAGTGACCTTATCGGGCGCCACGGTGGCGAAGAGTTTCTGGTTGCCCTCCCAAATACCTCACTGGT
AGAAGCCCGTACCTTGATTGACACGATTCGCACCAGTTTTGCTGCACTCCGTCACTATGGGCCTGCCGGCGAATTCACGG
TTACCTTCAGCGCGGGGATTGCCGGCTATCCACACCACCGTAGCCAGCTTGGTGTGATGCAGGCTGCCGATGCAGCCCTC
TACCAGGCCAAACACGCCGGTCGCAACCGCATCGTTGTTGACACAACCGCTGGCGATACACCGCCGCTCCTGCTACCAGC
CGCAAAAGACGAAGCAACCATCCGCACCGAGGCACCCTCCTGGCGGGCTTTGATCATCAGTGCTAATGGCATTCAACGTC
GCATGCTGCGCCAGGCGCTGACTGCCCAGGGTTTGATGTACGATATGATCGATGATGTTGAACGTGCCTTACAGTATGAA
GCGCCAATCCCACCCAACCTCATTCTGGTCGATATGACGCAGATTGAAGGGGCACGCACTGCGAATATCATTCTGTTGCG
CGAACACTTTGCAAAGGGACGTATTGTCACCATCGCCCCGGCAACCGCCGAACCACGTGCCGCGGCTACAGTTGCTTTCG
GTGTCGATGATTATCTCCTCATTCCACTGGCTGCCGGCGCGTTATCAGCCTTGCTCGATCGCCTGAGTGCATCAGAGAGG
AGCACGTAG

Upstream 100 bases:

>100_bases
GTCGAACAGTATCTGGCACTAGGGGCAGTTGATGTTATCTCCAAACCCTTTGATCCAATGACCTTATCAGAACAAGTTGC
AGCAATCTGGTTGAAAGCTA

Downstream 100 bases:

>100_bases
ATGAAACCCCATACACTGCTGATCGTTGATGATGACGATACCGTTGCCGAGATGCTCGAACTGGTGCTGCGTGGTGCCGG
TTACGAAGTGCGGCGTGCTG

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MKPDPAEQFEALRKRYAAQIGERVAAIHAAWNQLVQSDWDKTAGVELRQLAHKLAGSAAMFGFANLGAVAHDIDLRLKEW
LEHDQSLDTLARQHLAQQIALLQHAVGEYRTSTAVPETNQPTPSTAPSEPPPVPPANTEPAPPIPQPQPPATPREPNGRP
QTIVLVEDDREFAAKLVGQLRLFNYDVHLVNDLEGLPTLIRAINPAAIIMDIVFPEGDLAGTETIANLQRERTEPIPVIY
ISQRDDFTARLAAVRSGGHAYFAKPVDAGQLINALDRLTGVEENTPLRVLIVDDDQEFGHNCVHSLEIAGIHARAITDPR
ETLNQLQVFQPDLIVLDMYMPICSGKELASIIRQQDRYVGMPIVFLSAETNRLQQQSVLSLGADDFLAKPISPADLVAAI
SARAMRARYLQRFLIRDSLTGLLNHQAVEEELSHEFARAERLQQPLSIAIIDIDHFKRVNDSYGHLIGDQVLRSFARLLQ
QRLRKSDLIGRHGGEEFLVALPNTSLVEARTLIDTIRTSFAALRHYGPAGEFTVTFSAGIAGYPHHRSQLGVMQAADAAL
YQAKHAGRNRIVVDTTAGDTPPLLLPAAKDEATIRTEAPSWRALIISANGIQRRMLRQALTAQGLMYDMIDDVERALQYE
APIPPNLILVDMTQIEGARTANIILLREHFAKGRIVTIAPATAEPRAAATVAFGVDDYLLIPLAAGALSALLDRLSASER
ST

Sequences:

>Translated_722_residues
MKPDPAEQFEALRKRYAAQIGERVAAIHAAWNQLVQSDWDKTAGVELRQLAHKLAGSAAMFGFANLGAVAHDIDLRLKEW
LEHDQSLDTLARQHLAQQIALLQHAVGEYRTSTAVPETNQPTPSTAPSEPPPVPPANTEPAPPIPQPQPPATPREPNGRP
QTIVLVEDDREFAAKLVGQLRLFNYDVHLVNDLEGLPTLIRAINPAAIIMDIVFPEGDLAGTETIANLQRERTEPIPVIY
ISQRDDFTARLAAVRSGGHAYFAKPVDAGQLINALDRLTGVEENTPLRVLIVDDDQEFGHNCVHSLEIAGIHARAITDPR
ETLNQLQVFQPDLIVLDMYMPICSGKELASIIRQQDRYVGMPIVFLSAETNRLQQQSVLSLGADDFLAKPISPADLVAAI
SARAMRARYLQRFLIRDSLTGLLNHQAVEEELSHEFARAERLQQPLSIAIIDIDHFKRVNDSYGHLIGDQVLRSFARLLQ
QRLRKSDLIGRHGGEEFLVALPNTSLVEARTLIDTIRTSFAALRHYGPAGEFTVTFSAGIAGYPHHRSQLGVMQAADAAL
YQAKHAGRNRIVVDTTAGDTPPLLLPAAKDEATIRTEAPSWRALIISANGIQRRMLRQALTAQGLMYDMIDDVERALQYE
APIPPNLILVDMTQIEGARTANIILLREHFAKGRIVTIAPATAEPRAAATVAFGVDDYLLIPLAAGALSALLDRLSASER
ST
>Mature_722_residues
MKPDPAEQFEALRKRYAAQIGERVAAIHAAWNQLVQSDWDKTAGVELRQLAHKLAGSAAMFGFANLGAVAHDIDLRLKEW
LEHDQSLDTLARQHLAQQIALLQHAVGEYRTSTAVPETNQPTPSTAPSEPPPVPPANTEPAPPIPQPQPPATPREPNGRP
QTIVLVEDDREFAAKLVGQLRLFNYDVHLVNDLEGLPTLIRAINPAAIIMDIVFPEGDLAGTETIANLQRERTEPIPVIY
ISQRDDFTARLAAVRSGGHAYFAKPVDAGQLINALDRLTGVEENTPLRVLIVDDDQEFGHNCVHSLEIAGIHARAITDPR
ETLNQLQVFQPDLIVLDMYMPICSGKELASIIRQQDRYVGMPIVFLSAETNRLQQQSVLSLGADDFLAKPISPADLVAAI
SARAMRARYLQRFLIRDSLTGLLNHQAVEEELSHEFARAERLQQPLSIAIIDIDHFKRVNDSYGHLIGDQVLRSFARLLQ
QRLRKSDLIGRHGGEEFLVALPNTSLVEARTLIDTIRTSFAALRHYGPAGEFTVTFSAGIAGYPHHRSQLGVMQAADAAL
YQAKHAGRNRIVVDTTAGDTPPLLLPAAKDEATIRTEAPSWRALIISANGIQRRMLRQALTAQGLMYDMIDDVERALQYE
APIPPNLILVDMTQIEGARTANIILLREHFAKGRIVTIAPATAEPRAAATVAFGVDDYLLIPLAAGALSALLDRLSASER
ST

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=167, Percent_Identity=39.5209580838323, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=37.1794871794872, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081881, Length=156, Percent_Identity=41.025641025641, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=35.4037267080745, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1787541, Length=160, Percent_Identity=35.625, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=36.969696969697, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=35.9756097560976, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=35.4430379746835, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787802, Length=199, Percent_Identity=32.1608040201005, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI87081977, Length=149, Percent_Identity=35.5704697986577, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1788394, Length=128, Percent_Identity=32.8125, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081974, Length=156, Percent_Identity=30.1282051282051, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=27.4390243902439, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=33.0434782608696, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 79329; Mature: 79329

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPDPAEQFEALRKRYAAQIGERVAAIHAAWNQLVQSDWDKTAGVELRQLAHKLAGSAAM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHCCCHHH
FGFANLGAVAHDIDLRLKEWLEHDQSLDTLARQHLAQQIALLQHAVGEYRTSTAVPETNQ
HHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PTPSTAPSEPPPVPPANTEPAPPIPQPQPPATPREPNGRPQTIVLVEDDREFAAKLVGQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
RLFNYDVHLVNDLEGLPTLIRAINPAAIIMDIVFPEGDLAGTETIANLQRERTEPIPVIY
HHEEEEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEE
ISQRDDFTARLAAVRSGGHAYFAKPVDAGQLINALDRLTGVEENTPLRVLIVDDDQEFGH
EECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHH
NCVHSLEIAGIHARAITDPRETLNQLQVFQPDLIVLDMYMPICSGKELASIIRQQDRYVG
HHHHHHHCCCEEHHCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHCCCCC
MPIVFLSAETNRLQQQSVLSLGADDFLAKPISPADLVAAISARAMRARYLQRFLIRDSLT
CCEEEEECCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLLNHQAVEEELSHEFARAERLQQPLSIAIIDIDHFKRVNDSYGHLIGDQVLRSFARLLQ
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QRLRKSDLIGRHGGEEFLVALPNTSLVEARTLIDTIRTSFAALRHYGPAGEFTVTFSAGI
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
AGYPHHRSQLGVMQAADAALYQAKHAGRNRIVVDTTAGDTPPLLLPAAKDEATIRTEAPS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCEEEECCCC
WRALIISANGIQRRMLRQALTAQGLMYDMIDDVERALQYEAPIPPNLILVDMTQIEGART
CEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHCCCCCC
ANIILLREHFAKGRIVTIAPATAEPRAAATVAFGVDDYLLIPLAAGALSALLDRLSASER
EEEEEEEHHHCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
ST
CC
>Mature Secondary Structure
MKPDPAEQFEALRKRYAAQIGERVAAIHAAWNQLVQSDWDKTAGVELRQLAHKLAGSAAM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHCCCHHH
FGFANLGAVAHDIDLRLKEWLEHDQSLDTLARQHLAQQIALLQHAVGEYRTSTAVPETNQ
HHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PTPSTAPSEPPPVPPANTEPAPPIPQPQPPATPREPNGRPQTIVLVEDDREFAAKLVGQL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHH
RLFNYDVHLVNDLEGLPTLIRAINPAAIIMDIVFPEGDLAGTETIANLQRERTEPIPVIY
HHEEEEEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEE
ISQRDDFTARLAAVRSGGHAYFAKPVDAGQLINALDRLTGVEENTPLRVLIVDDDQEFGH
EECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHH
NCVHSLEIAGIHARAITDPRETLNQLQVFQPDLIVLDMYMPICSGKELASIIRQQDRYVG
HHHHHHHCCCEEHHCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCHHHHHHHHHHCCCCC
MPIVFLSAETNRLQQQSVLSLGADDFLAKPISPADLVAAISARAMRARYLQRFLIRDSLT
CCEEEEECCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLLNHQAVEEELSHEFARAERLQQPLSIAIIDIDHFKRVNDSYGHLIGDQVLRSFARLLQ
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHH
QRLRKSDLIGRHGGEEFLVALPNTSLVEARTLIDTIRTSFAALRHYGPAGEFTVTFSAGI
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
AGYPHHRSQLGVMQAADAALYQAKHAGRNRIVVDTTAGDTPPLLLPAAKDEATIRTEAPS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCCEEEECCCC
WRALIISANGIQRRMLRQALTAQGLMYDMIDDVERALQYEAPIPPNLILVDMTQIEGART
CEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHCCCCCC
ANIILLREHFAKGRIVTIAPATAEPRAAATVAFGVDDYLLIPLAAGALSALLDRLSASER
EEEEEEEHHHCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
ST
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA