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The map label for this gene is yhfT [H]

Identifier: 222527291

GI number: 222527291

Start: 5045963

End: 5047309

Strand: Direct

Name: yhfT [H]

Synonym: Chy400_4076

Alternate gene names: 222527291

Gene position: 5045963-5047309 (Clockwise)

Preceding gene: 222527290

Following gene: 222527292

Centisome position: 95.77

GC content: 55.46

Gene sequence:

>1347_bases
ATGAGCATTGCATGGCTACGCGAACGGATGGAGCAATGGGCAAGCCACCCGGCTATCGTCTGGCATGACCATCCGTATCC
ATACCAGGACTTGTTAACGCGCATACAAGCCTGGCACACCGCTCTCAACCAGCACGACGTCGGTCCCGGCGAGGTCGTAA
CGATTGAAGGAGATTATTCACCCAATGCCGTTAGCCTGCTCCTGACCCTGATCGAACGCAATACGATTGTTGTTCCGTTG
ACGCACAGTGTTGCAGCGCAGCGTGAAGAGTTTCTGGGGATCGCCGAAGTGCAGGTCGTTGTCAGTTTCACCGACGATGA
CCGCTGGCATATCGAGCGCCGACCGGTGCAGGTAACCAATCCGCTGACCCGTCAGTTGATCGACCGTCAGCATCCGGGAT
TAGTCCTCTTCTCGTCGGGTTCTACCGGGAAGAGCAAAGCTGCCTTACACGATTTTATTCCGCTGCTCGAAAAGTTCAAA
GTTCCCCGCCACCGACGGGTGACACTCACCTTCCTGCTGCTCGATCATATCGGCGGTATCAATACCCTCTTCTACACCCT
GGCAAATGGCGGCACTGTGGTATCGGTACCGTCACGCGATCCCGACGTAGTCTGTCGGGCTATTGAAACCCACCGGGTGC
AGACGTTGCCCACGTCGCCGACGTTTCTGAATCTCCTGTTGATCTCTGAAGCCTACCGGCGCTATGATCTCTCGTCACTT
GAGCTGATTACTTACGGCACAGAGGTGATGCCGGAAAGTACCTTACACCGGATTCACGCGCTCTTCCCCAACGTGCAGCT
CTTGCAGACGTATGGCCTCTCTGAACTGGGCATCCTGCGTTCCAAGTCCCGCGACTCCAATTCACTATGGGTCAAAGTCG
GCGGCGAAGGGTTCGAGACGAAGATCGTCGATGGGGTGTTGTGGGTACGGGCTAAATCGGCGATGCTGGGGTATCTCAAT
GCACCCAGCCCCTTCGACGAAGAGGGGTGGATGAATACGCAGGATGTGGTTGAGGTTGACGGAGAGTATATTCGCATTTT
GGGGCGACGGAGCGACATCATCAACGTCGGTGGGCAGAAGGTCTACCCGGCAGAGGTGGAGAGCGTGTTACTCCAGATGC
CGAACGTCAAAGATGTTGCGGTGGTTGGTGAGCCTAATCCCATCACCGGCCATATTGTGACTGCACGCTTCAATCTGTTC
GAGCCTGAAGACCCGAATGAGTTTAAGCGGCGGGTACGTGCATTCTGTCGTGACCGCCTGGCACCTTACAAGATACCGGT
CAAGATTATCATTACCGACAGCGAACAACACAGCGCCCGGTTCAAGAAGATGCGTAAATCGTCGTAG

Upstream 100 bases:

>100_bases
GTGATTGATTTCTTCGTCAACCCGGCCAGTGATTATGTCACCGGGCAGGTACTTTACCTTGGTGGTGGTGGGTAGCATCG
ATCACCGCATGGAGGCCGCT

Downstream 100 bases:

>100_bases
CAGCTTTGCAGCGAAAAACGAAGCCTATGATACCGACAGTTCGCATTGCTATCGATCCGGCACTGGCGGCCCGGCGCGCT
GAGATTACCTATACCTGGCG

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSIAWLRERMEQWASHPAIVWHDHPYPYQDLLTRIQAWHTALNQHDVGPGEVVTIEGDYSPNAVSLLLTLIERNTIVVPL
THSVAAQREEFLGIAEVQVVVSFTDDDRWHIERRPVQVTNPLTRQLIDRQHPGLVLFSSGSTGKSKAALHDFIPLLEKFK
VPRHRRVTLTFLLLDHIGGINTLFYTLANGGTVVSVPSRDPDVVCRAIETHRVQTLPTSPTFLNLLLISEAYRRYDLSSL
ELITYGTEVMPESTLHRIHALFPNVQLLQTYGLSELGILRSKSRDSNSLWVKVGGEGFETKIVDGVLWVRAKSAMLGYLN
APSPFDEEGWMNTQDVVEVDGEYIRILGRRSDIINVGGQKVYPAEVESVLLQMPNVKDVAVVGEPNPITGHIVTARFNLF
EPEDPNEFKRRVRAFCRDRLAPYKIPVKIIITDSEQHSARFKKMRKSS

Sequences:

>Translated_448_residues
MSIAWLRERMEQWASHPAIVWHDHPYPYQDLLTRIQAWHTALNQHDVGPGEVVTIEGDYSPNAVSLLLTLIERNTIVVPL
THSVAAQREEFLGIAEVQVVVSFTDDDRWHIERRPVQVTNPLTRQLIDRQHPGLVLFSSGSTGKSKAALHDFIPLLEKFK
VPRHRRVTLTFLLLDHIGGINTLFYTLANGGTVVSVPSRDPDVVCRAIETHRVQTLPTSPTFLNLLLISEAYRRYDLSSL
ELITYGTEVMPESTLHRIHALFPNVQLLQTYGLSELGILRSKSRDSNSLWVKVGGEGFETKIVDGVLWVRAKSAMLGYLN
APSPFDEEGWMNTQDVVEVDGEYIRILGRRSDIINVGGQKVYPAEVESVLLQMPNVKDVAVVGEPNPITGHIVTARFNLF
EPEDPNEFKRRVRAFCRDRLAPYKIPVKIIITDSEQHSARFKKMRKSS
>Mature_447_residues
SIAWLRERMEQWASHPAIVWHDHPYPYQDLLTRIQAWHTALNQHDVGPGEVVTIEGDYSPNAVSLLLTLIERNTIVVPLT
HSVAAQREEFLGIAEVQVVVSFTDDDRWHIERRPVQVTNPLTRQLIDRQHPGLVLFSSGSTGKSKAALHDFIPLLEKFKV
PRHRRVTLTFLLLDHIGGINTLFYTLANGGTVVSVPSRDPDVVCRAIETHRVQTLPTSPTFLNLLLISEAYRRYDLSSLE
LITYGTEVMPESTLHRIHALFPNVQLLQTYGLSELGILRSKSRDSNSLWVKVGGEGFETKIVDGVLWVRAKSAMLGYLNA
PSPFDEEGWMNTQDVVEVDGEYIRILGRRSDIINVGGQKVYPAEVESVLLQMPNVKDVAVVGEPNPITGHIVTARFNLFE
PEDPNEFKRRVRAFCRDRLAPYKIPVKIIITDSEQHSARFKKMRKSS

Specific function: May be involved in fatty acid metabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=371, Percent_Identity=29.3800539083558, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI187761343, Length=371, Percent_Identity=29.3800539083558, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI58082049, Length=340, Percent_Identity=24.1176470588235, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI38505220, Length=338, Percent_Identity=27.810650887574, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI157311624, Length=335, Percent_Identity=23.2835820895522, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157311622, Length=335, Percent_Identity=23.2835820895522, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI156151445, Length=342, Percent_Identity=24.2690058479532, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI115511026, Length=342, Percent_Identity=26.9005847953216, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788107, Length=339, Percent_Identity=28.3185840707965, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI145693145, Length=329, Percent_Identity=26.7477203647416, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786810, Length=267, Percent_Identity=27.7153558052434, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788595, Length=324, Percent_Identity=26.5432098765432, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71996755, Length=332, Percent_Identity=28.3132530120482, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=304, Percent_Identity=29.9342105263158, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=451, Percent_Identity=23.9467849223947, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=448, Percent_Identity=23.4375, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=343, Percent_Identity=25.0728862973761, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=474, Percent_Identity=24.2616033755274, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=337, Percent_Identity=25.2225519287834, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25141359, Length=451, Percent_Identity=22.6164079822616, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=477, Percent_Identity=23.6897274633124, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994694, Length=360, Percent_Identity=25, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=360, Percent_Identity=25, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=360, Percent_Identity=25, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=287, Percent_Identity=25.4355400696864, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=350, Percent_Identity=24, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581924, Length=345, Percent_Identity=25.7971014492754, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355181, Length=284, Percent_Identity=27.112676056338, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281365686, Length=306, Percent_Identity=23.202614379085, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356947, Length=279, Percent_Identity=26.5232974910394, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356441, Length=281, Percent_Identity=27.7580071174377, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648260, Length=282, Percent_Identity=23.4042553191489, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24653035, Length=269, Percent_Identity=24.907063197026, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI161076582, Length=268, Percent_Identity=24.2537313432836, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21358303, Length=257, Percent_Identity=25.6809338521401, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648257, Length=307, Percent_Identity=26.7100977198697, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 50716; Mature: 50585

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAWLRERMEQWASHPAIVWHDHPYPYQDLLTRIQAWHTALNQHDVGPGEVVTIEGDYS
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
PNAVSLLLTLIERNTIVVPLTHSVAAQREEFLGIAEVQVVVSFTDDDRWHIERRPVQVTN
CHHHHHHHHHHHCCEEEEEECHHHHHHHHHHCCEEEEEEEEEECCCCCEEEECCCEEECC
PLTRQLIDRQHPGLVLFSSGSTGKSKAALHDFIPLLEKFKVPRHRRVTLTFLLLDHIGGI
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHCCCH
NTLFYTLANGGTVVSVPSRDPDVVCRAIETHRVQTLPTSPTFLNLLLISEAYRRYDLSSL
HHHHEEECCCCEEEECCCCCHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHCCCCCE
ELITYGTEVMPESTLHRIHALFPNVQLLQTYGLSELGILRSKSRDSNSLWVKVGGEGFET
EEEECCCHHCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCCCCH
KIVDGVLWVRAKSAMLGYLNAPSPFDEEGWMNTQDVVEVDGEYIRILGRRSDIINVGGQK
HHHHHHHEEEEHHHHEEECCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEECCCCE
VYPAEVESVLLQMPNVKDVAVVGEPNPITGHIVTARFNLFEPEDPNEFKRRVRAFCRDRL
ECHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCC
APYKIPVKIIITDSEQHSARFKKMRKSS
CCEECEEEEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIAWLRERMEQWASHPAIVWHDHPYPYQDLLTRIQAWHTALNQHDVGPGEVVTIEGDYS
CHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCC
PNAVSLLLTLIERNTIVVPLTHSVAAQREEFLGIAEVQVVVSFTDDDRWHIERRPVQVTN
CHHHHHHHHHHHCCEEEEEECHHHHHHHHHHCCEEEEEEEEEECCCCCEEEECCCEEECC
PLTRQLIDRQHPGLVLFSSGSTGKSKAALHDFIPLLEKFKVPRHRRVTLTFLLLDHIGGI
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEHHHCCCH
NTLFYTLANGGTVVSVPSRDPDVVCRAIETHRVQTLPTSPTFLNLLLISEAYRRYDLSSL
HHHHEEECCCCEEEECCCCCHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHCCCCCE
ELITYGTEVMPESTLHRIHALFPNVQLLQTYGLSELGILRSKSRDSNSLWVKVGGEGFET
EEEECCCHHCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHCCCCCCCEEEEEECCCCCCH
KIVDGVLWVRAKSAMLGYLNAPSPFDEEGWMNTQDVVEVDGEYIRILGRRSDIINVGGQK
HHHHHHHEEEEHHHHEEECCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEECCCCE
VYPAEVESVLLQMPNVKDVAVVGEPNPITGHIVTARFNLFEPEDPNEFKRRVRAFCRDRL
ECHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCC
APYKIPVKIIITDSEQHSARFKKMRKSS
CCEECEEEEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]