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The map label for this gene is tgt [H]

Identifier: 222527245

GI number: 222527245

Start: 4978720

End: 4979937

Strand: Reverse

Name: tgt [H]

Synonym: Chy400_4029

Alternate gene names: 222527245

Gene position: 4979937-4978720 (Counterclockwise)

Preceding gene: 222527246

Following gene: 222527240

Centisome position: 94.51

GC content: 63.14

Gene sequence:

>1218_bases
ATGGCACCATTTACCATCACCGCTCGTGATCCAGAGAGCCGCGCTCGCGCCGGTCTTCTCCAAACTGCCCATGGCACGGT
CGCCACACCGGTCTTCATGCCGGTCGGCACGCGGGCAACCGTCAAATCCCTCACCCCCCACGAACTCCGTGACCATGGCG
CATCGATCATTCTGGGCAACACCTACCACCTCTACCTCCAGCCCGGCCACGAATTGATCGCCCGTCACGGTGGCCTGCAC
AGCTTCATGGGCTGGAACGGCCCGATTCTGACCGACAGTGGCGGCTTTCAGGTCTTCTCACTCGTCTACGGCGGGATTGC
CGATGAAGTGAAAGGTCGTCGTCCTGCCCATCCCACGCGCCTCAACGACATGGTTACCGTCACCGAAGAGGCGGTCATCT
TCAAGTCATACCTTGACGGTTCACGCCACGTCTTCACCCCCGAACGCAGCATCGAAGTACAGCACCACCTCGGCGCCGAC
ATCATCGTCTGCTTCGACGAACTGCCACCATTTCGCGCCGGCTACGACTATACCGCTCAAAGCATGGCCCGCACCCACCG
CTGGGCCGAGCGCTGCCTGATCGCGCACCAACGCCGGGATCGGTCGGCCTTACCCAACCCCGATCAACTACTCTTCGGAA
TCGTCCACGGCGGCATCTTCCCCGATCTACGCCGGGCCAGCGCCTGCTACATCAGCGACATGGCCTTCGACGGCCTCTGC
ATCGGCGGCTCGCTCGGTGCCAACAAAACCCAGATGTACGAAGTGGTTGATATGACCGTGCCCTACCTGCCCGACGGCAT
GGCCCGCCACCTGCTCGGCGTCGGCGACGTTGACGACCTGCTCGAAGGGGTGGCGCGTGGGATCGACATGTTCGACTGCG
TCAGCCCAACCCGGCTTGGCCGCCACGGGGCAGCCCTGATCCGCAACCGCGAGCGCAACTGGCGGCTCAACGTCACCAAC
GCCGCCCTCCGCGACGACCGGCAGCCACTGCAAGAGGGCTGCCCCTGCTACACCTGCCGCCACTTCTCACGCGCCTACAT
CCATCACCTCTACCGCTCAAAAGAGCTACTCGGCATCAGACTGGTGAGCCTGCACAACGTCGCATTCTTGCTCAACCTGA
TGGCCGACATCCGCAGCGCCCTGGCTGCCGGACGCTTCGGCGAACTCTACACCGAATGGTTGGGCAAACCGCTACCGGTT
TTACCGTCGCCCGAATAG

Upstream 100 bases:

>100_bases
GGACAATAGCACTTATTGTTGATCAGCGATGGTAGTCGGGTATAATACCGACCTGTGTTGATAACAACCTGATAGTCGTA
TCAGCTACGAAATAAACTGT

Downstream 100 bases:

>100_bases
CCTCTCCTCATCCCCAGTACGACCGGATCATGATGGGTGGAATGGGCATCGCCATCAGACACGATACACTCGGCGATGCC
CAAGCCACCCCACCTCTGCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MAPFTITARDPESRARAGLLQTAHGTVATPVFMPVGTRATVKSLTPHELRDHGASIILGNTYHLYLQPGHELIARHGGLH
SFMGWNGPILTDSGGFQVFSLVYGGIADEVKGRRPAHPTRLNDMVTVTEEAVIFKSYLDGSRHVFTPERSIEVQHHLGAD
IIVCFDELPPFRAGYDYTAQSMARTHRWAERCLIAHQRRDRSALPNPDQLLFGIVHGGIFPDLRRASACYISDMAFDGLC
IGGSLGANKTQMYEVVDMTVPYLPDGMARHLLGVGDVDDLLEGVARGIDMFDCVSPTRLGRHGAALIRNRERNWRLNVTN
AALRDDRQPLQEGCPCYTCRHFSRAYIHHLYRSKELLGIRLVSLHNVAFLLNLMADIRSALAAGRFGELYTEWLGKPLPV
LPSPE

Sequences:

>Translated_405_residues
MAPFTITARDPESRARAGLLQTAHGTVATPVFMPVGTRATVKSLTPHELRDHGASIILGNTYHLYLQPGHELIARHGGLH
SFMGWNGPILTDSGGFQVFSLVYGGIADEVKGRRPAHPTRLNDMVTVTEEAVIFKSYLDGSRHVFTPERSIEVQHHLGAD
IIVCFDELPPFRAGYDYTAQSMARTHRWAERCLIAHQRRDRSALPNPDQLLFGIVHGGIFPDLRRASACYISDMAFDGLC
IGGSLGANKTQMYEVVDMTVPYLPDGMARHLLGVGDVDDLLEGVARGIDMFDCVSPTRLGRHGAALIRNRERNWRLNVTN
AALRDDRQPLQEGCPCYTCRHFSRAYIHHLYRSKELLGIRLVSLHNVAFLLNLMADIRSALAAGRFGELYTEWLGKPLPV
LPSPE
>Mature_404_residues
APFTITARDPESRARAGLLQTAHGTVATPVFMPVGTRATVKSLTPHELRDHGASIILGNTYHLYLQPGHELIARHGGLHS
FMGWNGPILTDSGGFQVFSLVYGGIADEVKGRRPAHPTRLNDMVTVTEEAVIFKSYLDGSRHVFTPERSIEVQHHLGADI
IVCFDELPPFRAGYDYTAQSMARTHRWAERCLIAHQRRDRSALPNPDQLLFGIVHGGIFPDLRRASACYISDMAFDGLCI
GGSLGANKTQMYEVVDMTVPYLPDGMARHLLGVGDVDDLLEGVARGIDMFDCVSPTRLGRHGAALIRNRERNWRLNVTNA
ALRDDRQPLQEGCPCYTCRHFSRAYIHHLYRSKELLGIRLVSLHNVAFLLNLMADIRSALAAGRFGELYTEWLGKPLPVL
PSPE

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=391, Percent_Identity=39.8976982097187, Blast_Score=275, Evalue=4e-74,
Organism=Escherichia coli, GI1786607, Length=394, Percent_Identity=42.6395939086294, Blast_Score=318, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17544680, Length=381, Percent_Identity=38.0577427821522, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI25146502, Length=136, Percent_Identity=30.1470588235294, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920510, Length=402, Percent_Identity=38.3084577114428, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI21355311, Length=161, Percent_Identity=27.9503105590062, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 44994; Mature: 44863

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPFTITARDPESRARAGLLQTAHGTVATPVFMPVGTRATVKSLTPHELRDHGASIILGN
CCCEEEEECCCHHHHHHHHHHHCCCCEECCEEECCCCCHHHHCCCHHHHHHCCCEEEECC
TYHLYLQPGHELIARHGGLHSFMGWNGPILTDSGGFQVFSLVYGGIADEVKGRRPAHPTR
EEEEEECCCHHHHHHCCCCHHHCCCCCCEEECCCCHHHHHHHHCCHHHHHCCCCCCCCCC
LNDMVTVTEEAVIFKSYLDGSRHVFTPERSIEVQHHLGADIIVCFDELPPFRAGYDYTAQ
CCCEEEEHHHHHHHHHHCCCCCEEECCCCCEEEHHHCCCEEEEEECCCCCCCCCCCHHHH
SMARTHRWAERCLIAHQRRDRSALPNPDQLLFGIVHGGIFPDLRRASACYISDMAFDGLC
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEE
IGGSLGANKTQMYEVVDMTVPYLPDGMARHLLGVGDVDDLLEGVARGIDMFDCVSPTRLG
ECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCHHHHCCHHHHC
RHGAALIRNRERNWRLNVTNAALRDDRQPLQEGCPCYTCRHFSRAYIHHLYRSKELLGIR
CCHHHHHHCCCCCEEEEECHHHHHCCHHHHHHCCCEEHHHHHHHHHHHHHHHCCHHHEEE
LVSLHNVAFLLNLMADIRSALAAGRFGELYTEWLGKPLPVLPSPE
EHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
APFTITARDPESRARAGLLQTAHGTVATPVFMPVGTRATVKSLTPHELRDHGASIILGN
CCEEEEECCCHHHHHHHHHHHCCCCEECCEEECCCCCHHHHCCCHHHHHHCCCEEEECC
TYHLYLQPGHELIARHGGLHSFMGWNGPILTDSGGFQVFSLVYGGIADEVKGRRPAHPTR
EEEEEECCCHHHHHHCCCCHHHCCCCCCEEECCCCHHHHHHHHCCHHHHHCCCCCCCCCC
LNDMVTVTEEAVIFKSYLDGSRHVFTPERSIEVQHHLGADIIVCFDELPPFRAGYDYTAQ
CCCEEEEHHHHHHHHHHCCCCCEEECCCCCEEEHHHCCCEEEEEECCCCCCCCCCCHHHH
SMARTHRWAERCLIAHQRRDRSALPNPDQLLFGIVHGGIFPDLRRASACYISDMAFDGLC
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEE
IGGSLGANKTQMYEVVDMTVPYLPDGMARHLLGVGDVDDLLEGVARGIDMFDCVSPTRLG
ECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCHHHHCCHHHHC
RHGAALIRNRERNWRLNVTNAALRDDRQPLQEGCPCYTCRHFSRAYIHHLYRSKELLGIR
CCHHHHHHCCCCCEEEEECHHHHHCCHHHHHHCCCEEHHHHHHHHHHHHHHHCCHHHEEE
LVSLHNVAFLLNLMADIRSALAAGRFGELYTEWLGKPLPVLPSPE
EHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA