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The map label for this gene is cofE [H]

Identifier: 222527118

GI number: 222527118

Start: 4848624

End: 4849226

Strand: Reverse

Name: cofE [H]

Synonym: Chy400_3899

Alternate gene names: 222527118

Gene position: 4849226-4848624 (Counterclockwise)

Preceding gene: 222527119

Following gene: 222527116

Centisome position: 92.03

GC content: 61.53

Gene sequence:

>603_bases
ATGGATTTTTTTACGCTTGTTCAGACCCGGCGGACGGTACGGTCGTTCCGTCCCGATCCGCCGCCGCGTGCGGCGATTGA
GCAAATCCTCCACGCTGCCAGTTGGGCGCCATCGCCGCACGGTCGCCAGCCCTGGCGCTTTGTAGTGGTTGAGTCGCTCG
AACGGAAACAGCTTCTGGCGCAGGCGATGGGGGCAGCCTGGCGTCATCACCTGGCCCTCGATGGTCTTGATCCGGCAACT
ATCGAGGTGCGGTTACAACGCTCGCAAGAGCGCGTGCTGAACGCGCCGGTCATTATCGTGCCCTGTCTCTACCTGAACGA
TCTCGACACCTATCCCGACGCTGACCGTCAGGCTGCTGAGACGACGATGGCGATCCAGAGTTTGGGTTGTGCTGTCCAAA
ACATGTTGCTGGCCGCGTTTGCTCTCGGCCTGGCTGCAGGCTGGATGTGTGCTCCGCTGTTTGCACCGGCAGCCGTGCGT
ACTGCACTGGCCTTGGCCGACGATCTGCATCCCCAGGCGCTGATACCGATTGGCTACCTTGCGCAAGAACCAAAACGGCG
TCCGCGGCGTCCGCTGCACGAGCTGATCGTTCAGTGGTTGTAA

Upstream 100 bases:

>100_bases
CCGGCGGCGATTATTCGTGGCTATCACTATCTACCCAGTCAGACTGCTACTGCTCGTCAGTTGATCCGCGATCCGCGGTT
TGATCTCTTTCGGTAAGCCA

Downstream 100 bases:

>100_bases
TGCTCTGGCTGGTTACGTCGCGGTCTAATGAAGATGGGGATGATCGCAGCGTACAGCTCAAACATCGTCGAAATCAGCAC
TGTCTGGCGTAATCACACAA

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MDFFTLVQTRRTVRSFRPDPPPRAAIEQILHAASWAPSPHGRQPWRFVVVESLERKQLLAQAMGAAWRHHLALDGLDPAT
IEVRLQRSQERVLNAPVIIVPCLYLNDLDTYPDADRQAAETTMAIQSLGCAVQNMLLAAFALGLAAGWMCAPLFAPAAVR
TALALADDLHPQALIPIGYLAQEPKRRPRRPLHELIVQWL

Sequences:

>Translated_200_residues
MDFFTLVQTRRTVRSFRPDPPPRAAIEQILHAASWAPSPHGRQPWRFVVVESLERKQLLAQAMGAAWRHHLALDGLDPAT
IEVRLQRSQERVLNAPVIIVPCLYLNDLDTYPDADRQAAETTMAIQSLGCAVQNMLLAAFALGLAAGWMCAPLFAPAAVR
TALALADDLHPQALIPIGYLAQEPKRRPRRPLHELIVQWL
>Mature_200_residues
MDFFTLVQTRRTVRSFRPDPPPRAAIEQILHAASWAPSPHGRQPWRFVVVESLERKQLLAQAMGAAWRHHLALDGLDPAT
IEVRLQRSQERVLNAPVIIVPCLYLNDLDTYPDADRQAAETTMAIQSLGCAVQNMLLAAFALGLAAGWMCAPLFAPAAVR
TALALADDLHPQALIPIGYLAQEPKRRPRRPLHELIVQWL

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225
- InterPro:   IPR019943
- InterPro:   IPR000415 [H]

Pfam domain/function: PF01996 F420_ligase; PF00881 Nitroreductase [H]

EC number: NA

Molecular weight: Translated: 22315; Mature: 22315

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFFTLVQTRRTVRSFRPDPPPRAAIEQILHAASWAPSPHGRQPWRFVVVESLERKQLLA
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
QAMGAAWRHHLALDGLDPATIEVRLQRSQERVLNAPVIIVPCLYLNDLDTYPDADRQAAE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCCHHHHHH
TTMAIQSLGCAVQNMLLAAFALGLAAGWMCAPLFAPAAVRTALALADDLHPQALIPIGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHH
AQEPKRRPRRPLHELIVQWL
HCCHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MDFFTLVQTRRTVRSFRPDPPPRAAIEQILHAASWAPSPHGRQPWRFVVVESLERKQLLA
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
QAMGAAWRHHLALDGLDPATIEVRLQRSQERVLNAPVIIVPCLYLNDLDTYPDADRQAAE
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCCHHHHHH
TTMAIQSLGCAVQNMLLAAFALGLAAGWMCAPLFAPAAVRTALALADDLHPQALIPIGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHH
AQEPKRRPRRPLHELIVQWL
HCCHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA