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The map label for this gene is cofE [H]

Identifier: 222527119

GI number: 222527119

Start: 4849231

End: 4849986

Strand: Reverse

Name: cofE [H]

Synonym: Chy400_3900

Alternate gene names: 222527119

Gene position: 4849986-4849231 (Counterclockwise)

Preceding gene: 222527120

Following gene: 222527118

Centisome position: 92.05

GC content: 59.66

Gene sequence:

>756_bases
ATGCAAGGTGAGATACGGATTTTGCCGTTGCGCGGGATCGGTGAGGTGCAGCCGGGTGATGACCTGGTGGCAATACTGGC
TACGGCTATCGATGCCGCAGGCGGCCTCGAACAGGGCGACATTCTGGTCGTCACGCAGAAGATTGTCTCGAAGGCCGAGG
GTCGTCTGGTCGATCCATCAACCATCGAGCCATCACCCTTTGCGCAAGAGATCGCCCGTACCGCGAAGAAAGATGCCCGT
TATCAAGAGGTGGTGCTGCGCGAAAGTAAGCGCATCGTGAAGATGGCGAATGGCGTCCTCATCACCGAAACCCGCCATGG
TCTGGTGTGTGCCAACAGTGGTGTTGACGAGTCAAATGTGGATGGTGGGCGGCGGCTGGCGTTGTTGCCGCTCGATCCCG
ACGCCAGTGCCGCTGCCCTGCGTGAAGGGTTGGCCCGGCGCTACGACTACGCACCAGCCGTAATTATTACCGACACCTTC
GGTCGGCCATGGCGCGAGGGACAGGTGAATGTGGCGATTGGGGTCGCCGGGATGCTGCCGCTCCACGATTTTGCCGGCGT
CGCCGATCCGTATGGGTACACGATGCAGGCAACGTTAATCGCTGTTGCCGATGAACTGGCTGCCGCCGCCGAGCTGGTCA
TGGGCAAGATAGATCGGGTGCCGGCGGCGATTATTCGTGGCTATCACTATCTACCCAGTCAGACTGCTACTGCTCGTCAG
TTGATCCGCGATCCGCGGTTTGATCTCTTTCGGTAA

Upstream 100 bases:

>100_bases
TGCACTACGCTATGCTATTGTTACTCATCCCGATCATATTCCGGCAGCCGTGAAAGAGCGTTTGGCCCCAATCGCCGGAC
GCTACTGGTCGTGAGGCGTT

Downstream 100 bases:

>100_bases
GCCAATGGATTTTTTTACGCTTGTTCAGACCCGGCGGACGGTACGGTCGTTCCGTCCCGATCCGCCGCCGCGTGCGGCGA
TTGAGCAAATCCTCCACGCT

Product: F420-dependent oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MQGEIRILPLRGIGEVQPGDDLVAILATAIDAAGGLEQGDILVVTQKIVSKAEGRLVDPSTIEPSPFAQEIARTAKKDAR
YQEVVLRESKRIVKMANGVLITETRHGLVCANSGVDESNVDGGRRLALLPLDPDASAAALREGLARRYDYAPAVIITDTF
GRPWREGQVNVAIGVAGMLPLHDFAGVADPYGYTMQATLIAVADELAAAAELVMGKIDRVPAAIIRGYHYLPSQTATARQ
LIRDPRFDLFR

Sequences:

>Translated_251_residues
MQGEIRILPLRGIGEVQPGDDLVAILATAIDAAGGLEQGDILVVTQKIVSKAEGRLVDPSTIEPSPFAQEIARTAKKDAR
YQEVVLRESKRIVKMANGVLITETRHGLVCANSGVDESNVDGGRRLALLPLDPDASAAALREGLARRYDYAPAVIITDTF
GRPWREGQVNVAIGVAGMLPLHDFAGVADPYGYTMQATLIAVADELAAAAELVMGKIDRVPAAIIRGYHYLPSQTATARQ
LIRDPRFDLFR
>Mature_251_residues
MQGEIRILPLRGIGEVQPGDDLVAILATAIDAAGGLEQGDILVVTQKIVSKAEGRLVDPSTIEPSPFAQEIARTAKKDAR
YQEVVLRESKRIVKMANGVLITETRHGLVCANSGVDESNVDGGRRLALLPLDPDASAAALREGLARRYDYAPAVIITDTF
GRPWREGQVNVAIGVAGMLPLHDFAGVADPYGYTMQATLIAVADELAAAAELVMGKIDRVPAAIIRGYHYLPSQTATARQ
LIRDPRFDLFR

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 27007; Mature: 27007

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGEIRILPLRGIGEVQPGDDLVAILATAIDAAGGLEQGDILVVTQKIVSKAEGRLVDPS
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCCCCCC
TIEPSPFAQEIARTAKKDARYQEVVLRESKRIVKMANGVLITETRHGLVCANSGVDESNV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCCC
DGGRRLALLPLDPDASAAALREGLARRYDYAPAVIITDTFGRPWREGQVNVAIGVAGMLP
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEEEECCCC
LHDFAGVADPYGYTMQATLIAVADELAAAAELVMGKIDRVPAAIIRGYHYLPSQTATARQ
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LIRDPRFDLFR
HHHCCCCCCCC
>Mature Secondary Structure
MQGEIRILPLRGIGEVQPGDDLVAILATAIDAAGGLEQGDILVVTQKIVSKAEGRLVDPS
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHCCCCCCCC
TIEPSPFAQEIARTAKKDARYQEVVLRESKRIVKMANGVLITETRHGLVCANSGVDESNV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCCC
DGGRRLALLPLDPDASAAALREGLARRYDYAPAVIITDTFGRPWREGQVNVAIGVAGMLP
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEEEEEEEECCCC
LHDFAGVADPYGYTMQATLIAVADELAAAAELVMGKIDRVPAAIIRGYHYLPSQTATARQ
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LIRDPRFDLFR
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]