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The map label for this gene is gcvH [H]

Identifier: 222526990

GI number: 222526990

Start: 4688362

End: 4688751

Strand: Direct

Name: gcvH [H]

Synonym: Chy400_3768

Alternate gene names: 222526990

Gene position: 4688362-4688751 (Clockwise)

Preceding gene: 222526989

Following gene: 222526991

Centisome position: 88.98

GC content: 57.18

Gene sequence:

>390_bases
ATGGCTTACAACACTCCATCCGATCTACGGTACAGCAAAACCCACGAGTGGGTACGACAGGAGGGTGATGAGGTCGTGAT
TGGGATTACCGATTATGCCCAGCACGCGCTCGGCGATGTGGTGTACATCGAACTGCCGGCAGTCGGCTCGACGATTACCG
TCGGCGAGAGCTTCGGGATTGTCGAGTCGGTGAAGGCCAGTTCAGATCTGTACGCCCCAGTAAGTGGCGAAGTGGTGGCG
GTGAATACTGCGCTCGAAAGCGATCAGGCGCCGATCAACAGTGATCCGTATGGTGCGGGCTGGATGTTGCGCGTGCGCCC
CAGTGGCGAATCGGCTGAATTGCTCGATGCCGAGGCGTATGCTGCGTTTGTTGCCGAAATCGATCATTAA

Upstream 100 bases:

>100_bases
GTAGTGAATTTGATGTCATCGTGCGCGAGCGTCCGGTACGTGCGCGCGTGGTAAAGATGCCTTTCTATAAGCCGCGCTAC
AAGCGCTAGAGGAGTTCGAC

Downstream 100 bases:

>100_bases
ACGTTAGCTAGATGGAAGCTGGTATGACCCACTACATTCCGATTACCGATGCCGACCGCCAGGCGATGCTACAGGCAATC
GGTGTTGAAACAATTGAAGA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MAYNTPSDLRYSKTHEWVRQEGDEVVIGITDYAQHALGDVVYIELPAVGSTITVGESFGIVESVKASSDLYAPVSGEVVA
VNTALESDQAPINSDPYGAGWMLRVRPSGESAELLDAEAYAAFVAEIDH

Sequences:

>Translated_129_residues
MAYNTPSDLRYSKTHEWVRQEGDEVVIGITDYAQHALGDVVYIELPAVGSTITVGESFGIVESVKASSDLYAPVSGEVVA
VNTALESDQAPINSDPYGAGWMLRVRPSGESAELLDAEAYAAFVAEIDH
>Mature_128_residues
AYNTPSDLRYSKTHEWVRQEGDEVVIGITDYAQHALGDVVYIELPAVGSTITVGESFGIVESVKASSDLYAPVSGEVVAV
NTALESDQAPINSDPYGAGWMLRVRPSGESAELLDAEAYAAFVAEIDH

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=122, Percent_Identity=40.9836065573771, Blast_Score=105, Evalue=1e-23,
Organism=Homo sapiens, GI89057342, Length=122, Percent_Identity=40.1639344262295, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1789271, Length=124, Percent_Identity=50.8064516129032, Blast_Score=134, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=49.5652173913044, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=46.6101694915254, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=102, Percent_Identity=44.1176470588235, Blast_Score=101, Evalue=4e-23,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=40.3361344537815, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13820; Mature: 13689

Theoretical pI: Translated: 3.92; Mature: 3.92

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYNTPSDLRYSKTHEWVRQEGDEVVIGITDYAQHALGDVVYIELPAVGSTITVGESFGI
CCCCCCCCCEECHHHHHHHCCCCEEEEEEHHHHHHHHCCEEEEEECCCCCEEEECCCCCC
VESVKASSDLYAPVSGEVVAVNTALESDQAPINSDPYGAGWMLRVRPSGESAELLDAEAY
HHHHCCCCCEECCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCEECHHHH
AAFVAEIDH
HEEEEECCC
>Mature Secondary Structure 
AYNTPSDLRYSKTHEWVRQEGDEVVIGITDYAQHALGDVVYIELPAVGSTITVGESFGI
CCCCCCCCEECHHHHHHHCCCCEEEEEEHHHHHHHHCCEEEEEECCCCCEEEECCCCCC
VESVKASSDLYAPVSGEVVAVNTALESDQAPINSDPYGAGWMLRVRPSGESAELLDAEAY
HHHHCCCCCEECCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCEECHHHH
AAFVAEIDH
HEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA