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The map label for this gene is sseA [H]

Identifier: 222526978

GI number: 222526978

Start: 4677905

End: 4678756

Strand: Direct

Name: sseA [H]

Synonym: Chy400_3756

Alternate gene names: 222526978

Gene position: 4677905-4678756 (Clockwise)

Preceding gene: 222526977

Following gene: 222526979

Centisome position: 88.78

GC content: 56.22

Gene sequence:

>852_bases
ATGAGCGGTTACGCCCATCCCGAAGCACTGGTCGAGACCCAGTGGGTCGCCGATCACCTCAATGATCCCAATGTTCGCAT
CGTCGAGAGCGATGAAGATCTGTTGCTGTACGATACCGGTCACATTCCCGGCGCGGTGAAGATTGACTGGGTGCAAGACT
TGAACGATCCGGTTGTTCGTGATTATCTTGACTCTGATCGGTTTGCCGCACTGATGCGGGTAAAGGGCATCAGTAACGAT
ACTACGGTCGTTCTGTACGGTGATAAGCACAACTGGTGGGCAACCTATGCCTTTTGGGTCTTTAAACTCTTCGGTCATCG
TGATGTGCGCATTATGAACGGTGGGCGGGCGAAGTGGATTGCTGAGGGTCGTCCGTTGACGCGCGAAGTTCCCTCGTATC
CACCAGGAAATTATGTCGCGCCACCGCGCAATGACGCTGAAATTCGGGCATTTCGCGATCAGGTGCTGGAGTTTGTCCGT
CAGCGCAAAGGTGTTCTGGTCGATGTCCGTAGCCCGCAAGAGTATACCGGCGAACGCACCCATATGCCCGATTATCCGCA
AGAGGGCACGCTACGCGGCGGCCACATTCCCACCGCGGTCAACATTCCCTGGGCCAGGGCGGTGAATGAGGATAGCACCT
TCAAGAGCGTCGAGGAGCTACGCGAGTTGTACGCCGCTCAGGGTGTTACCCCCGACAAAGAGGTTATCGCCTACTGCCGG
ATTGGTGAGCGGAGCAGCCATACCTGGTTTGTGCTGACCTACTTGCTGGGCTTCCCCAATGTCCGCAATTACGATGGGAG
CTGGACGGAGTGGGGCAACAGCGTTGGTCTGCCCATCGAGAAGAATGTGTAG

Upstream 100 bases:

>100_bases
GTTTTCTGATTAACAACTTGACTAATGGAGTAAAACACGCTACAATGGCAGTGTCGGTAGGTCGCTACAGGCACTGCTAT
TTGCTAAGGTGGAGGTTTCT

Downstream 100 bases:

>100_bases
AGCGTAGCGTAAACAGCTCTACTAAAGATCGTCTCAGCGGGTGAGGGGTGACTCATGTGTCGCCCCTGCAATGTCTCAGG
TAGGTGTAAGGGTCGCACAA

Product: Rhodanese domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSGYAHPEALVETQWVADHLNDPNVRIVESDEDLLLYDTGHIPGAVKIDWVQDLNDPVVRDYLDSDRFAALMRVKGISND
TTVVLYGDKHNWWATYAFWVFKLFGHRDVRIMNGGRAKWIAEGRPLTREVPSYPPGNYVAPPRNDAEIRAFRDQVLEFVR
QRKGVLVDVRSPQEYTGERTHMPDYPQEGTLRGGHIPTAVNIPWARAVNEDSTFKSVEELRELYAAQGVTPDKEVIAYCR
IGERSSHTWFVLTYLLGFPNVRNYDGSWTEWGNSVGLPIEKNV

Sequences:

>Translated_283_residues
MSGYAHPEALVETQWVADHLNDPNVRIVESDEDLLLYDTGHIPGAVKIDWVQDLNDPVVRDYLDSDRFAALMRVKGISND
TTVVLYGDKHNWWATYAFWVFKLFGHRDVRIMNGGRAKWIAEGRPLTREVPSYPPGNYVAPPRNDAEIRAFRDQVLEFVR
QRKGVLVDVRSPQEYTGERTHMPDYPQEGTLRGGHIPTAVNIPWARAVNEDSTFKSVEELRELYAAQGVTPDKEVIAYCR
IGERSSHTWFVLTYLLGFPNVRNYDGSWTEWGNSVGLPIEKNV
>Mature_282_residues
SGYAHPEALVETQWVADHLNDPNVRIVESDEDLLLYDTGHIPGAVKIDWVQDLNDPVVRDYLDSDRFAALMRVKGISNDT
TVVLYGDKHNWWATYAFWVFKLFGHRDVRIMNGGRAKWIAEGRPLTREVPSYPPGNYVAPPRNDAEIRAFRDQVLEFVRQ
RKGVLVDVRSPQEYTGERTHMPDYPQEGTLRGGHIPTAVNIPWARAVNEDSTFKSVEELRELYAAQGVTPDKEVIAYCRI
GERSSHTWFVLTYLLGFPNVRNYDGSWTEWGNSVGLPIEKNV

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=278, Percent_Identity=33.8129496402878, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI194473668, Length=284, Percent_Identity=30.2816901408451, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI61835204, Length=284, Percent_Identity=30.2816901408451, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI194473681, Length=284, Percent_Identity=30.2816901408451, Blast_Score=111, Evalue=6e-25,
Organism=Escherichia coli, GI87082121, Length=282, Percent_Identity=31.2056737588652, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI87081967, Length=289, Percent_Identity=27.681660899654, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17561888, Length=298, Percent_Identity=25.1677852348993, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI115534702, Length=218, Percent_Identity=31.1926605504587, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71997283, Length=255, Percent_Identity=24.7058823529412, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 32360; Mature: 32228

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGYAHPEALVETQWVADHLNDPNVRIVESDEDLLLYDTGHIPGAVKIDWVQDLNDPVVR
CCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCEEEEEHHHCCCCHHHH
DYLDSDRFAALMRVKGISNDTTVVLYGDKHNWWATYAFWVFKLFGHRDVRIMNGGRAKWI
HHHCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEE
AEGRPLTREVPSYPPGNYVAPPRNDAEIRAFRDQVLEFVRQRKGVLVDVRSPQEYTGERT
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCC
HMPDYPQEGTLRGGHIPTAVNIPWARAVNEDSTFKSVEELRELYAAQGVTPDKEVIAYCR
CCCCCCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEEE
IGERSSHTWFVLTYLLGFPNVRNYDGSWTEWGNSVGLPIEKNV
CCCCCCCCEEEEEHHHCCCCCCCCCCCHHHCCCCCCCEECCCC
>Mature Secondary Structure 
SGYAHPEALVETQWVADHLNDPNVRIVESDEDLLLYDTGHIPGAVKIDWVQDLNDPVVR
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCEEEEEHHHCCCCHHHH
DYLDSDRFAALMRVKGISNDTTVVLYGDKHNWWATYAFWVFKLFGHRDVRIMNGGRAKWI
HHHCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEE
AEGRPLTREVPSYPPGNYVAPPRNDAEIRAFRDQVLEFVRQRKGVLVDVRSPQEYTGERT
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCC
HMPDYPQEGTLRGGHIPTAVNIPWARAVNEDSTFKSVEELRELYAAQGVTPDKEVIAYCR
CCCCCCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEEE
IGERSSHTWFVLTYLLGFPNVRNYDGSWTEWGNSVGLPIEKNV
CCCCCCCCEEEEEHHHCCCCCCCCCCCHHHCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]