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The map label for this gene is glnA [H]

Identifier: 222526882

GI number: 222526882

Start: 4561121

End: 4562455

Strand: Direct

Name: glnA [H]

Synonym: Chy400_3659

Alternate gene names: 222526882

Gene position: 4561121-4562455 (Clockwise)

Preceding gene: 222526881

Following gene: 222526887

Centisome position: 86.57

GC content: 59.18

Gene sequence:

>1335_bases
ATGAGTGATGATCCGCGCACCGCGCTCCTGCATCGCGTCGAGGCCGATCAGGTGGCATTCATTAATCTGCAATTCACCGA
CGTGCTGGGTATCGGGAAGACGGTGACCATTCCGGTTGAGGAGCTGCCCGATGCGCTTGACCACGGCGTCTGGTTTGATG
GCTCTTCTATCGAAGGCTTTGCCCGGATGGTCGAGAGTGATATGTATCTTGTGCCCGACATCTCGACCTACGCCGTCGTG
CCATGGGATCAGCATCAGGGTGTTACCACCGCCCGCTTGATCTGCTCGGTCTATACTCCCGATGGCAAACCGTTTGCCGG
CGATCCGCGTAATGTGCTGCGACGGATGCTCGATCAGGCTGCCGCCGCCGGTTATCGGTTTATGGTTGCGCCTGAACTAG
AGTTTTTTCTCTTCAAGACCGATCCGAACGGCAACATTCTTGAACCACACGATAAGGCCAGCTATTTCGATATATCCACC
GATCTGGCGACGCATATTCGCCGCCAGATGGCCCGTACCTTGCAAGCGATGGGGATTGCCGTTGAAGCGGTGCACCACGA
GGTGGCGCCCGGCCAGCACGAGATCGATCTGCGCTACGCCGATGCCTTGCAGTCGGCAGATCACCTGGTGACGGCACGGG
TGGCGCTGAAGGCGGTTGCCCAAATGAATGGTCTCCACGCAACCTTTATGCCCAAGCCTATCGCCGGTGTCAATGGCAGC
GGGATGCATGTCCATCAGAGTCTGCTCGACCGCGATACGGGGAAGAATCTCTTCGCCGATCCAGATGATCCCTACGGCCT
TTCGGCGTTGGCTCGCCACTTTATCGCCGGTCTGCTGGCGCACGCCCGTGGGATGTGCGCCCTGCTTGCCCCGCTAGTCA
ATTCGTACAAGCGCCTGGTTCCTGGATTTGAGGCGCCGGTCTATATCTCGTGGGGTCGTACCAACCGCTCGGCACTGGTA
CGTGTGCCACGCATTACTGCCGGACGCTACCAGGCAACCCGGATTGAACTCCGCTGCCCCGATCCAGCCTGTAATCCCTA
TCTGGCCTACACCGCGATGCTGGCCGCCGGTCTCGATGGGATTCGGCGCAAGTTGCCGTTGCGCGATGCAACCGAAGAAG
ACCTCTTCCACGTCGATCCGCGTGCCCGTGGGCTGACCACGCTGCCAACGTCACTAGGTTCGGCACTTGACGCCCTGCGC
GAAGATGAAGTTATCCTCGAAGCCATTGGCCCCTCAATCGCCGAGCGGTTTCTTGATGCCCGTCAGCAGGAGTGGGAAAG
CTACCGCGCCTATGTCTCGCAGTGGGAGATTGAGCGCTATCTGGCAATTTTCTAG

Upstream 100 bases:

>100_bases
GAACCCAATTGCCTCCCCCGCTCCGTCGCCATCCTGGCCCGTAGTATGGTATAGTACAGAAAATACCTGTACGACGAAAC
ACAAGTAAGGAGCATCGCTC

Downstream 100 bases:

>100_bases
GATGGGTATGCAATGAGACGGAGCGCACGACCATGCGCTCCGTTTTTGTGTGGTCGTCAGATCACAATCGAGCTGACGGC
AATAAAATGGCAGAGGCTAC

Product: glutamine synthetase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSDDPRTALLHRVEADQVAFINLQFTDVLGIGKTVTIPVEELPDALDHGVWFDGSSIEGFARMVESDMYLVPDISTYAVV
PWDQHQGVTTARLICSVYTPDGKPFAGDPRNVLRRMLDQAAAAGYRFMVAPELEFFLFKTDPNGNILEPHDKASYFDIST
DLATHIRRQMARTLQAMGIAVEAVHHEVAPGQHEIDLRYADALQSADHLVTARVALKAVAQMNGLHATFMPKPIAGVNGS
GMHVHQSLLDRDTGKNLFADPDDPYGLSALARHFIAGLLAHARGMCALLAPLVNSYKRLVPGFEAPVYISWGRTNRSALV
RVPRITAGRYQATRIELRCPDPACNPYLAYTAMLAAGLDGIRRKLPLRDATEEDLFHVDPRARGLTTLPTSLGSALDALR
EDEVILEAIGPSIAERFLDARQQEWESYRAYVSQWEIERYLAIF

Sequences:

>Translated_444_residues
MSDDPRTALLHRVEADQVAFINLQFTDVLGIGKTVTIPVEELPDALDHGVWFDGSSIEGFARMVESDMYLVPDISTYAVV
PWDQHQGVTTARLICSVYTPDGKPFAGDPRNVLRRMLDQAAAAGYRFMVAPELEFFLFKTDPNGNILEPHDKASYFDIST
DLATHIRRQMARTLQAMGIAVEAVHHEVAPGQHEIDLRYADALQSADHLVTARVALKAVAQMNGLHATFMPKPIAGVNGS
GMHVHQSLLDRDTGKNLFADPDDPYGLSALARHFIAGLLAHARGMCALLAPLVNSYKRLVPGFEAPVYISWGRTNRSALV
RVPRITAGRYQATRIELRCPDPACNPYLAYTAMLAAGLDGIRRKLPLRDATEEDLFHVDPRARGLTTLPTSLGSALDALR
EDEVILEAIGPSIAERFLDARQQEWESYRAYVSQWEIERYLAIF
>Mature_443_residues
SDDPRTALLHRVEADQVAFINLQFTDVLGIGKTVTIPVEELPDALDHGVWFDGSSIEGFARMVESDMYLVPDISTYAVVP
WDQHQGVTTARLICSVYTPDGKPFAGDPRNVLRRMLDQAAAAGYRFMVAPELEFFLFKTDPNGNILEPHDKASYFDISTD
LATHIRRQMARTLQAMGIAVEAVHHEVAPGQHEIDLRYADALQSADHLVTARVALKAVAQMNGLHATFMPKPIAGVNGSG
MHVHQSLLDRDTGKNLFADPDDPYGLSALARHFIAGLLAHARGMCALLAPLVNSYKRLVPGFEAPVYISWGRTNRSALVR
VPRITAGRYQATRIELRCPDPACNPYLAYTAMLAAGLDGIRRKLPLRDATEEDLFHVDPRARGLTTLPTSLGSALDALRE
DEVILEAIGPSIAERFLDARQQEWESYRAYVSQWEIERYLAIF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=432, Percent_Identity=28.7037037037037, Blast_Score=164, Evalue=1e-40,
Organism=Escherichia coli, GI1790301, Length=473, Percent_Identity=36.1522198731501, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI87081870, Length=446, Percent_Identity=28.47533632287, Blast_Score=152, Evalue=3e-38,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49216; Mature: 49084

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDPRTALLHRVEADQVAFINLQFTDVLGIGKTVTIPVEELPDALDHGVWFDGSSIEGF
CCCCHHHHHHHHCCCCCEEEEEEEEHHHHCCCCEEEEEHHHCCHHHHCCEEECCCCHHHH
ARMVESDMYLVPDISTYAVVPWDQHQGVTTARLICSVYTPDGKPFAGDPRNVLRRMLDQA
HHHHHCCCEEECCCCEEEEECCCCCCCCCHHEEEEEEECCCCCCCCCCHHHHHHHHHHHH
AAAGYRFMVAPELEFFLFKTDPNGNILEPHDKASYFDISTDLATHIRRQMARTLQAMGIA
HHCCEEEEEECCEEEEEEEECCCCCEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
VEAVHHEVAPGQHEIDLRYADALQSADHLVTARVALKAVAQMNGLHATFMPKPIAGVNGS
HHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
GMHVHQSLLDRDTGKNLFADPDDPYGLSALARHFIAGLLAHARGMCALLAPLVNSYKRLV
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGFEAPVYISWGRTNRSALVRVPRITAGRYQATRIELRCPDPACNPYLAYTAMLAAGLDG
CCCCCCEEEEECCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
IRRKLPLRDATEEDLFHVDPRARGLTTLPTSLGSALDALREDEVILEAIGPSIAERFLDA
HHHCCCCCCCCCCCCEECCCCCCCCEECHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHH
RQQEWESYRAYVSQWEIERYLAIF
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDDPRTALLHRVEADQVAFINLQFTDVLGIGKTVTIPVEELPDALDHGVWFDGSSIEGF
CCCHHHHHHHHCCCCCEEEEEEEEHHHHCCCCEEEEEHHHCCHHHHCCEEECCCCHHHH
ARMVESDMYLVPDISTYAVVPWDQHQGVTTARLICSVYTPDGKPFAGDPRNVLRRMLDQA
HHHHHCCCEEECCCCEEEEECCCCCCCCCHHEEEEEEECCCCCCCCCCHHHHHHHHHHHH
AAAGYRFMVAPELEFFLFKTDPNGNILEPHDKASYFDISTDLATHIRRQMARTLQAMGIA
HHCCEEEEEECCEEEEEEEECCCCCEECCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHH
VEAVHHEVAPGQHEIDLRYADALQSADHLVTARVALKAVAQMNGLHATFMPKPIAGVNGS
HHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
GMHVHQSLLDRDTGKNLFADPDDPYGLSALARHFIAGLLAHARGMCALLAPLVNSYKRLV
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PGFEAPVYISWGRTNRSALVRVPRITAGRYQATRIELRCPDPACNPYLAYTAMLAAGLDG
CCCCCCEEEEECCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
IRRKLPLRDATEEDLFHVDPRARGLTTLPTSLGSALDALREDEVILEAIGPSIAERFLDA
HHHCCCCCCCCCCCCEECCCCCCCCEECHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHH
RQQEWESYRAYVSQWEIERYLAIF
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11016950 [H]