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The map label for this gene is acsA1 [H]

Identifier: 222526881

GI number: 222526881

Start: 4558551

End: 4560542

Strand: Direct

Name: acsA1 [H]

Synonym: Chy400_3658

Alternate gene names: 222526881

Gene position: 4558551-4560542 (Clockwise)

Preceding gene: 222526880

Following gene: 222526882

Centisome position: 86.52

GC content: 60.14

Gene sequence:

>1992_bases
ATGACCACCGCAGCAAAGTTGTTGTGGGAGCCGCCGGAAGACCTGCGCGCCAATTGCACGATGCGTGCGTTTATGCGTTG
GTTAGAGCAGCGCAGCGGCTTACACTTCGCTGATTACCACCAGTTGTACCGCTGGTCGGTCGATCGCCTTGAGGACTTCT
GGGCAGCATTGTGGGAGTATTACCAGATCAAGGCTCATACACCCTACACGACGGTGCTGTCGAGTCGCGAGATGCCCGGC
GCACGCTGGTTCACCGGTGCCCATCTCAATTACGCCGAACACGTGTTTCGGCACGCTACCGATCAGCGACCGGCAGTGAT
ATTCGCTTCCGAACGCCAGGAACCAACACCTCTGAGCTGGGCGAGTCTGCGTGCGCAAACGGCGGCCCTGGCACAGACCC
TACGCGAGGCTGGAGTCGGGCCGGGGGATCGCGTCGTGGCCTATGTGCCGAATACGCCACACGCCCTCATCGGTTGTCTG
GCAACCGCGAGTCTGGGCGCGATCTGGTCGAGCTGTTCACCCGATTTCGGCAGCCCCAGCGTCATCGACCGCTTCAGTCA
AATTGCGCCGAAGGTCTTGATCGCGGTTGACGGCTATCAGTATGGTGGCAAGGCATTTGACCGCCGGGCCGAAGTTGCGG
CGATTCAGGCGGCCCTGCCCGATCTGGAGCTGACCATCTTTATCCCCTACCTCGATCCAACCGCCGAAGCAAGCGGCCTG
CGAGGGCGCGTCATCCGCTGGGAAGACGCCTTACGGCAGGAAGCCGAACTTCACTTCACCCCGGTGGAATTCAACGACCC
GCTGTGGGTGCTCTATTCATCGGGCACAACCGGCCTCCCCAAACCGATTGTCCATAGCCAGGGCGGTATCCTGCTCGAAC
ATATCAAATCCCTCGATCTCCACTTCGACATGCGAGCGGGTGATACCTTCTTCTGGTACACCACCACCGGCTGGATGATG
TGGAACTTCTTGATCGGTGGCCTGCTGATCGGCGCGATACCGATCCTCTACGATGGCAGCCCGGCCTATCCAGATATGGG
CGTCCTATGGCGCCTGGCCGAACAGACACGGATTCGTTACTTCGGCACCAGCGCCGGCTACATCACCGCCCTGATGAAAA
GTGGGGTCGAACCGGGCACTCAGTTCGATCTGAGCAGCATCAAAGCCATCGGCTCGACCGGCTCACCCTTACCGCCAGAA
GGGTTTGATTGGGTCTACGAACACATCAAACACGACGTCTGGCTGGTCTCGTACAGTGGCGGCACCGACGTATGCAGCGG
CTTTGTCGGTGGTTGTCCTCTCCTGCCGGTGTACAGCGGCGAAATTCAATGCCGCATCCTGGGCTGCCGGGCAGAAGCCT
ACGATACCGATGGGCAGAGTGTTACCGGCGTCATGGGCGAACTGGTCATCACCGCGCCAATGCCGTCGATGCCGATCTAC
TTCTGGAACGATCCCGACAACGCTCGTTACAAGGCCAGCTACTTCGAGCACTATCCCGGCGTCTGGCGGCATGGCGACTG
GATCGTGATTAACGAGCGGGGTGGCGTGATTATTTATGGCCGTTCGGACAGCACGATCAACCGCCAGGGTGTGCGCATGG
GCACCAGCGAAATCTACCGGGCGGTAGAGACGATCCCCGAAGTGCTCGACAGTCTGGTCATCGATCTCGAAGGATTGGGT
GGACGGTCATACATGCCGCTCTTCGTCGTCTTGCGCGAAGGGGTCACCCTCGACGACGATCTGCGTCAACGGATTAAGCA
GGTCATTCGCCAGACCCTCTCGCCCCGCCACGTTCCCGACGACATCTTCCAGATTCCGGCGGTTCCCCTGACCCTGAGCG
GGAAGAAGCTGGAGGTGCCGGTGAAGAAGATTCTGATGGGTGTACCGGTCGAGCGGGCGGCCAATCCCGACTCGTTGCGC
AACCCCGAATCGCTTCAGTTCTTCGTTGAACTGGCACGCACGCTGGCGAATAGCGACAAAGTGGCCGGGTGA

Upstream 100 bases:

>100_bases
ATCTTCTATCTCCTATCTCCTACCTCCTACATTTGACTCCTCCCTGCTCCTTCCACTACAATCGCTACAAACGCCAGTCA
ATACCAAACGGAGGCCAATG

Downstream 100 bases:

>100_bases
GCAATATTCACCACCGAGACACAGAGGGCACAGAGGATTTAGACATTCTCTATGCCCTCTGTACATCTATGTTTTGTCTA
TGTTCACCATTACGTCAGTG

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 663; Mature: 662

Protein sequence:

>663_residues
MTTAAKLLWEPPEDLRANCTMRAFMRWLEQRSGLHFADYHQLYRWSVDRLEDFWAALWEYYQIKAHTPYTTVLSSREMPG
ARWFTGAHLNYAEHVFRHATDQRPAVIFASERQEPTPLSWASLRAQTAALAQTLREAGVGPGDRVVAYVPNTPHALIGCL
ATASLGAIWSSCSPDFGSPSVIDRFSQIAPKVLIAVDGYQYGGKAFDRRAEVAAIQAALPDLELTIFIPYLDPTAEASGL
RGRVIRWEDALRQEAELHFTPVEFNDPLWVLYSSGTTGLPKPIVHSQGGILLEHIKSLDLHFDMRAGDTFFWYTTTGWMM
WNFLIGGLLIGAIPILYDGSPAYPDMGVLWRLAEQTRIRYFGTSAGYITALMKSGVEPGTQFDLSSIKAIGSTGSPLPPE
GFDWVYEHIKHDVWLVSYSGGTDVCSGFVGGCPLLPVYSGEIQCRILGCRAEAYDTDGQSVTGVMGELVITAPMPSMPIY
FWNDPDNARYKASYFEHYPGVWRHGDWIVINERGGVIIYGRSDSTINRQGVRMGTSEIYRAVETIPEVLDSLVIDLEGLG
GRSYMPLFVVLREGVTLDDDLRQRIKQVIRQTLSPRHVPDDIFQIPAVPLTLSGKKLEVPVKKILMGVPVERAANPDSLR
NPESLQFFVELARTLANSDKVAG

Sequences:

>Translated_663_residues
MTTAAKLLWEPPEDLRANCTMRAFMRWLEQRSGLHFADYHQLYRWSVDRLEDFWAALWEYYQIKAHTPYTTVLSSREMPG
ARWFTGAHLNYAEHVFRHATDQRPAVIFASERQEPTPLSWASLRAQTAALAQTLREAGVGPGDRVVAYVPNTPHALIGCL
ATASLGAIWSSCSPDFGSPSVIDRFSQIAPKVLIAVDGYQYGGKAFDRRAEVAAIQAALPDLELTIFIPYLDPTAEASGL
RGRVIRWEDALRQEAELHFTPVEFNDPLWVLYSSGTTGLPKPIVHSQGGILLEHIKSLDLHFDMRAGDTFFWYTTTGWMM
WNFLIGGLLIGAIPILYDGSPAYPDMGVLWRLAEQTRIRYFGTSAGYITALMKSGVEPGTQFDLSSIKAIGSTGSPLPPE
GFDWVYEHIKHDVWLVSYSGGTDVCSGFVGGCPLLPVYSGEIQCRILGCRAEAYDTDGQSVTGVMGELVITAPMPSMPIY
FWNDPDNARYKASYFEHYPGVWRHGDWIVINERGGVIIYGRSDSTINRQGVRMGTSEIYRAVETIPEVLDSLVIDLEGLG
GRSYMPLFVVLREGVTLDDDLRQRIKQVIRQTLSPRHVPDDIFQIPAVPLTLSGKKLEVPVKKILMGVPVERAANPDSLR
NPESLQFFVELARTLANSDKVAG
>Mature_662_residues
TTAAKLLWEPPEDLRANCTMRAFMRWLEQRSGLHFADYHQLYRWSVDRLEDFWAALWEYYQIKAHTPYTTVLSSREMPGA
RWFTGAHLNYAEHVFRHATDQRPAVIFASERQEPTPLSWASLRAQTAALAQTLREAGVGPGDRVVAYVPNTPHALIGCLA
TASLGAIWSSCSPDFGSPSVIDRFSQIAPKVLIAVDGYQYGGKAFDRRAEVAAIQAALPDLELTIFIPYLDPTAEASGLR
GRVIRWEDALRQEAELHFTPVEFNDPLWVLYSSGTTGLPKPIVHSQGGILLEHIKSLDLHFDMRAGDTFFWYTTTGWMMW
NFLIGGLLIGAIPILYDGSPAYPDMGVLWRLAEQTRIRYFGTSAGYITALMKSGVEPGTQFDLSSIKAIGSTGSPLPPEG
FDWVYEHIKHDVWLVSYSGGTDVCSGFVGGCPLLPVYSGEIQCRILGCRAEAYDTDGQSVTGVMGELVITAPMPSMPIYF
WNDPDNARYKASYFEHYPGVWRHGDWIVINERGGVIIYGRSDSTINRQGVRMGTSEIYRAVETIPEVLDSLVIDLEGLGG
RSYMPLFVVLREGVTLDDDLRQRIKQVIRQTLSPRHVPDDIFQIPAVPLTLSGKKLEVPVKKILMGVPVERAANPDSLRN
PESLQFFVELARTLANSDKVAG

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=660, Percent_Identity=40.4545454545455, Blast_Score=500, Evalue=1e-141,
Organism=Homo sapiens, GI28416953, Length=579, Percent_Identity=28.6701208981002, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI8923896, Length=661, Percent_Identity=26.0211800302572, Blast_Score=202, Evalue=8e-52,
Organism=Homo sapiens, GI253970489, Length=674, Percent_Identity=25.3709198813056, Blast_Score=198, Evalue=2e-50,
Organism=Homo sapiens, GI13375727, Length=629, Percent_Identity=26.7090620031796, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1790505, Length=649, Percent_Identity=25.2696456086287, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI1786529, Length=617, Percent_Identity=26.904376012966, Blast_Score=178, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17569507, Length=664, Percent_Identity=37.1987951807229, Blast_Score=436, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71982997, Length=635, Percent_Identity=26.6141732283465, Blast_Score=176, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71983001, Length=635, Percent_Identity=26.6141732283465, Blast_Score=176, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6323182, Length=637, Percent_Identity=28.2574568288854, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6319264, Length=649, Percent_Identity=27.4268104776579, Blast_Score=203, Evalue=6e-53,
Organism=Drosophila melanogaster, GI24667955, Length=623, Percent_Identity=25.5216693418941, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI62472339, Length=530, Percent_Identity=27.1698113207547, Blast_Score=193, Evalue=3e-49,
Organism=Drosophila melanogaster, GI21355225, Length=654, Percent_Identity=25.8409785932722, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24667959, Length=491, Percent_Identity=27.0875763747454, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 73900; Mature: 73769

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAAKLLWEPPEDLRANCTMRAFMRWLEQRSGLHFADYHQLYRWSVDRLEDFWAALWEY
CCCCHHHCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YQIKAHTPYTTVLSSREMPGARWFTGAHLNYAEHVFRHATDQRPAVIFASERQEPTPLSW
HEEEECCCHHHHHCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCH
ASLRAQTAALAQTLREAGVGPGDRVVAYVPNTPHALIGCLATASLGAIWSSCSPDFGSPS
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCH
VIDRFSQIAPKVLIAVDGYQYGGKAFDRRAEVAAIQAALPDLELTIFIPYLDPTAEASGL
HHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
RGRVIRWEDALRQEAELHFTPVEFNDPLWVLYSSGTTGLPKPIVHSQGGILLEHIKSLDL
CCCEEEHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCE
HFDMRAGDTFFWYTTTGWMMWNFLIGGLLIGAIPILYDGSPAYPDMGVLWRLAEQTRIRY
EEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCEEEE
FGTSAGYITALMKSGVEPGTQFDLSSIKAIGSTGSPLPPEGFDWVYEHIKHDVWLVSYSG
ECCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCCEEEEEECC
GTDVCSGFVGGCPLLPVYSGEIQCRILGCRAEAYDTDGQSVTGVMGELVITAPMPSMPIY
CHHHHHHHCCCCCCCEEECCCEEEEEEEEECCCCCCCCCCHHHHHCCEEEECCCCCCCEE
FWNDPDNARYKASYFEHYPGVWRHGDWIVINERGGVIIYGRSDSTINRQGVRMGTSEIYR
EECCCCCCCEEHHHHHHCCCCEECCCEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHHH
AVETIPEVLDSLVIDLEGLGGRSYMPLFVVLREGVTLDDDLRQRIKQVIRQTLSPRHVPD
HHHHHHHHHHHHHEEHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCH
DIFQIPAVPLTLSGKKLEVPVKKILMGVPVERAANPDSLRNPESLQFFVELARTLANSDK
HHHHCCCCEEEECCCEEECCHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC
VAG
CCC
>Mature Secondary Structure 
TTAAKLLWEPPEDLRANCTMRAFMRWLEQRSGLHFADYHQLYRWSVDRLEDFWAALWEY
CCCHHHCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YQIKAHTPYTTVLSSREMPGARWFTGAHLNYAEHVFRHATDQRPAVIFASERQEPTPLSW
HEEEECCCHHHHHCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCH
ASLRAQTAALAQTLREAGVGPGDRVVAYVPNTPHALIGCLATASLGAIWSSCSPDFGSPS
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCH
VIDRFSQIAPKVLIAVDGYQYGGKAFDRRAEVAAIQAALPDLELTIFIPYLDPTAEASGL
HHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
RGRVIRWEDALRQEAELHFTPVEFNDPLWVLYSSGTTGLPKPIVHSQGGILLEHIKSLDL
CCCEEEHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCE
HFDMRAGDTFFWYTTTGWMMWNFLIGGLLIGAIPILYDGSPAYPDMGVLWRLAEQTRIRY
EEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCEEEE
FGTSAGYITALMKSGVEPGTQFDLSSIKAIGSTGSPLPPEGFDWVYEHIKHDVWLVSYSG
ECCCHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCCEEEEEECC
GTDVCSGFVGGCPLLPVYSGEIQCRILGCRAEAYDTDGQSVTGVMGELVITAPMPSMPIY
CHHHHHHHCCCCCCCEEECCCEEEEEEEEECCCCCCCCCCHHHHHCCEEEECCCCCCCEE
FWNDPDNARYKASYFEHYPGVWRHGDWIVINERGGVIIYGRSDSTINRQGVRMGTSEIYR
EECCCCCCCEEHHHHHHCCCCEECCCEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHHH
AVETIPEVLDSLVIDLEGLGGRSYMPLFVVLREGVTLDDDLRQRIKQVIRQTLSPRHVPD
HHHHHHHHHHHHHEEHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCH
DIFQIPAVPLTLSGKKLEVPVKKILMGVPVERAANPDSLRNPESLQFFVELARTLANSDK
HHHHCCCCEEEECCCEEECCHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC
VAG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]