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The map label for this gene is ltrA [H]

Identifier: 222526871

GI number: 222526871

Start: 4538986

End: 4540290

Strand: Direct

Name: ltrA [H]

Synonym: Chy400_3647

Alternate gene names: 222526871

Gene position: 4538986-4540290 (Clockwise)

Preceding gene: 222526870

Following gene: 222526872

Centisome position: 86.15

GC content: 55.63

Gene sequence:

>1305_bases
ATGCTGGTCTTTCCACGCTATTCAGGCCCATCACTGATTGAACAGGTTTGCACGGTTGAAAATCTCACCAATGCCTGGCG
ACGTGTGCGCTCGAACATCAAAGTGTCGCGGCGTACTCGTAGTGCCGGGATTGATCAGATAACGCTGCACGATTTTGCCG
CCGACTGGCCGAATCAAATGGTTCGGCTGGCCGAGGAGCTACGTGATGGGAGCTATCGTCCATTACCGCCACGGCGGGTA
GCGATTGCCAAAGCGAGTGGCGGTGAACGGGCCATTGCGATTCTGACCATCCGTGATCGAATTGCGCAACGGGCCGTCCA
ACAGGTCTTAACGCCACTCTTTGAACCACTATTTCTCGACTGTTCGTATGGGTCGCGATTGGCAGTTGGTGTCCCGGAGG
CAATTGAACGAGTGGTTCGCTATACCGAACAAGGGTTGATCTGGGTTATTGATGGTGATATTCGTGCGTATTTTGACTCT
ATCGATCACGGGATTCTCCTAGGCCTGTTACGACAACGGATTGATGAACCGGCGATCCTGCATCTCATCGCTCAATGGCT
GGCCGTTGGCTCTGTCCATACTGAGACCCCAGACGAGACCCTGCCTGATAGTCCTCTCGTTGCGCTGCTGCGTCGGAGTG
GCGAATTGATCCATGAGGCATTAAACGCCCCATCCGATCCACTACCGACAGCGTATGACTATCCCGATCTCTCCAGGCCG
GCGTCGCCTCATTCCGGTATACCAACCGGTCTATTTGCTGCACTCAGCCTCGCTCAACCGGCATTCGAGATCGCTCGCCA
ACTGACCCCTCTACTGAAGCGGATCGGTGCCCAACGCCTGGCAGTAGGCGGCGCATTGGCTGTGGGGACAGTTCTGTTGA
GCGAGCTTGTCCATCGAGCACAGGCCAGCCATGATCGGCGTGGCACCTTGCAGGGTGGACCACTCTCGCCGTTGCTGGCA
AACATCTACCTCCATCCATTTGACCTGGCGATGACCGCGCACGGAGCCAGGATGGTACGCTTCGTTGACGACTTTGTCGT
GATGTGTCCTGATCGCACGACTGCTGAACACACCCTCGTTCTGGTAGAACGCCAACTGGCCACGTTGCGCTTAACGCTCA
ACCCCCAGAAGACCCGGATTGTGGCTTACGCTGGTGGAATTGAATTTCTCGGTCAGGCATTAGCACCGCGACGTCGGCAA
GGTTTTCTCCATGGAATCAGCGATTTTCATAGCGCTGAACAGCGGTTACGCGAACACGTTGAACGACTACGCAGACCAGA
CAAACCACCGACATCTGATCGATAA

Upstream 100 bases:

>100_bases
TACTAGCCACGAAAACGACCGTAGCTCTGCGATCCGGGGCTGGCATGTATCAGACACCGGGCAGCAGAATACCACAATAT
GAAAGTGAAGGAGTACAACC

Downstream 100 bases:

>100_bases
GACGGATATTCTGCCAATCAGGAGGTAGTGGATGGCTACGTTATACATTCAGGAACAGGGGACGACCGTGCGCAAACGGG
ACAATCAGATCATCATCATC

Product: RNA-directed DNA polymerase

Products: NA

Alternate protein names: Reverse-transcriptase; RNA maturase; DNA endonuclease [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MLVFPRYSGPSLIEQVCTVENLTNAWRRVRSNIKVSRRTRSAGIDQITLHDFAADWPNQMVRLAEELRDGSYRPLPPRRV
AIAKASGGERAIAILTIRDRIAQRAVQQVLTPLFEPLFLDCSYGSRLAVGVPEAIERVVRYTEQGLIWVIDGDIRAYFDS
IDHGILLGLLRQRIDEPAILHLIAQWLAVGSVHTETPDETLPDSPLVALLRRSGELIHEALNAPSDPLPTAYDYPDLSRP
ASPHSGIPTGLFAALSLAQPAFEIARQLTPLLKRIGAQRLAVGGALAVGTVLLSELVHRAQASHDRRGTLQGGPLSPLLA
NIYLHPFDLAMTAHGARMVRFVDDFVVMCPDRTTAEHTLVLVERQLATLRLTLNPQKTRIVAYAGGIEFLGQALAPRRRQ
GFLHGISDFHSAEQRLREHVERLRRPDKPPTSDR

Sequences:

>Translated_434_residues
MLVFPRYSGPSLIEQVCTVENLTNAWRRVRSNIKVSRRTRSAGIDQITLHDFAADWPNQMVRLAEELRDGSYRPLPPRRV
AIAKASGGERAIAILTIRDRIAQRAVQQVLTPLFEPLFLDCSYGSRLAVGVPEAIERVVRYTEQGLIWVIDGDIRAYFDS
IDHGILLGLLRQRIDEPAILHLIAQWLAVGSVHTETPDETLPDSPLVALLRRSGELIHEALNAPSDPLPTAYDYPDLSRP
ASPHSGIPTGLFAALSLAQPAFEIARQLTPLLKRIGAQRLAVGGALAVGTVLLSELVHRAQASHDRRGTLQGGPLSPLLA
NIYLHPFDLAMTAHGARMVRFVDDFVVMCPDRTTAEHTLVLVERQLATLRLTLNPQKTRIVAYAGGIEFLGQALAPRRRQ
GFLHGISDFHSAEQRLREHVERLRRPDKPPTSDR
>Mature_434_residues
MLVFPRYSGPSLIEQVCTVENLTNAWRRVRSNIKVSRRTRSAGIDQITLHDFAADWPNQMVRLAEELRDGSYRPLPPRRV
AIAKASGGERAIAILTIRDRIAQRAVQQVLTPLFEPLFLDCSYGSRLAVGVPEAIERVVRYTEQGLIWVIDGDIRAYFDS
IDHGILLGLLRQRIDEPAILHLIAQWLAVGSVHTETPDETLPDSPLVALLRRSGELIHEALNAPSDPLPTAYDYPDLSRP
ASPHSGIPTGLFAALSLAQPAFEIARQLTPLLKRIGAQRLAVGGALAVGTVLLSELVHRAQASHDRRGTLQGGPLSPLLA
NIYLHPFDLAMTAHGARMVRFVDDFVVMCPDRTTAEHTLVLVERQLATLRLTLNPQKTRIVAYAGGIEFLGQALAPRRRQ
GFLHGISDFHSAEQRLREHVERLRRPDKPPTSDR

Specific function: Multifunctional protein that promotes group II intron splicing and mobility by acting both on RNA and DNA. It has three activities:reverse transcriptase (RT) for intron duplication, maturase to promote splicing, and DNA endonuclease for site- specific cle

COG id: COG3344

COG function: function code L; Retron-type reverse transcriptase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 reverse transcriptase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6226521, Length=162, Percent_Identity=28.3950617283951, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6226520, Length=151, Percent_Identity=27.8145695364238, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR002866
- InterPro:   IPR000477 [H]

Pfam domain/function: PF01348 Intron_maturas2; PF00078 RVT_1 [H]

EC number: =2.7.7.49 [H]

Molecular weight: Translated: 48181; Mature: 48181

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50878 RT_POL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVFPRYSGPSLIEQVCTVENLTNAWRRVRSNIKVSRRTRSAGIDQITLHDFAADWPNQM
CEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEHHHHHHCCHHHH
VRLAEELRDGSYRPLPPRRVAIAKASGGERAIAILTIRDRIAQRAVQQVLTPLFEPLFLD
HHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEE
CSYGSRLAVGVPEAIERVVRYTEQGLIWVIDGDIRAYFDSIDHGILLGLLRQRIDEPAIL
CCCCCEEEECCHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHH
HLIAQWLAVGSVHTETPDETLPDSPLVALLRRSGELIHEALNAPSDPLPTAYDYPDLSRP
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
ASPHSGIPTGLFAALSLAQPAFEIARQLTPLLKRIGAQRLAVGGALAVGTVLLSELVHRA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
QASHDRRGTLQGGPLSPLLANIYLHPFDLAMTAHGARMVRFVDDFVVMCPDRTTAEHTLV
HHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHH
LVERQLATLRLTLNPQKTRIVAYAGGIEFLGQALAPRRRQGFLHGISDFHSAEQRLREHV
HEEHHHEEEEEEECCCCEEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ERLRRPDKPPTSDR
HHHHCCCCCCCCCC
>Mature Secondary Structure
MLVFPRYSGPSLIEQVCTVENLTNAWRRVRSNIKVSRRTRSAGIDQITLHDFAADWPNQM
CEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEHHHHHHCCHHHH
VRLAEELRDGSYRPLPPRRVAIAKASGGERAIAILTIRDRIAQRAVQQVLTPLFEPLFLD
HHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHEEE
CSYGSRLAVGVPEAIERVVRYTEQGLIWVIDGDIRAYFDSIDHGILLGLLRQRIDEPAIL
CCCCCEEEECCHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHH
HLIAQWLAVGSVHTETPDETLPDSPLVALLRRSGELIHEALNAPSDPLPTAYDYPDLSRP
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
ASPHSGIPTGLFAALSLAQPAFEIARQLTPLLKRIGAQRLAVGGALAVGTVLLSELVHRA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
QASHDRRGTLQGGPLSPLLANIYLHPFDLAMTAHGARMVRFVDDFVVMCPDRTTAEHTLV
HHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHH
LVERQLATLRLTLNPQKTRIVAYAGGIEFLGQALAPRRRQGFLHGISDFHSAEQRLREHV
HEEHHHEEEEEEECCCCEEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
ERLRRPDKPPTSDR
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8655550; 11157264; 10488339; 9353259; 10413481; 11731786 [H]