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The map label for this gene is dhaK [H]

Identifier: 222526780

GI number: 222526780

Start: 4427547

End: 4428545

Strand: Reverse

Name: dhaK [H]

Synonym: Chy400_3554

Alternate gene names: 222526780

Gene position: 4428545-4427547 (Counterclockwise)

Preceding gene: 222526781

Following gene: 222526779

Centisome position: 84.05

GC content: 57.86

Gene sequence:

>999_bases
GTGAAGAAGTTGATCAACGCACCGGAGAATGTCGTAAAAGAGGCATTAGCCGGAATGGCAATTGCCCATGCCGACCTGAT
TCAGGTTCACTACGATCCTGATTACATCGTGCGCGTCGGCGCACCACACAACAAGGTCGGTGTCATCTCCGGTGGCGGCT
CAGGCCACGAACCGATGCACGGTGGTTTTGTCGGCTACGGTATGCTCGATGCTGCCTGTCCCGGTGCCGTCTTTACCTCG
CCGGTGCCGGATCAGATGCTGGCGGCAACCAAGGCGGTGAACGGCGGGAAGGGTGTTTTGCACATCGTCAAAAACTACAC
CGGTGACGTGATGAACTTCGAGATGGCGGCTGAACTGGCCGCTGCCGAGGGCATTGAAGTTGCCAGTGTGGTGACAAACG
ATGACGTGGCGGTCGAAAACAGCACCTGGACGGCAGGTCGTCGTGGCGTGGGTGTGACTGTTCTGCTCGAGAAGATCGTT
GGGGCTGCGGCTGAGGCCGGTGCCGATCTGGCGACCTGTAAGGCAATTGCCGAGCGAGTCAATGCCAGTGGCCGCAGCAT
GGGGATGGCGCTAACACCCTGTACCGTACCCCAGGCCGGGAAGCCGGGTTTCGAGCTGGCCGATGATGAGATGGAAGTTG
GTATCGGCATTCACGGCGAACCAGGCCGGCGCCGCGAGAAGTTGGCTCCAGCACGGGATATTGCTGAGATGCTGGCTGGC
CCGATCCTGGAAGACCTGCCCTTCAAGAGCGGTGATGCGGTGCTGGCGTTTGTCAACGGTATGGGTGGCACACCGCTGAT
TGAGCTGTACGTGATGTACAATGAATTGGCACGTATCCTGAAGGATCGCAATATTACGATTGCTCGTTCACTGGTGGGAA
GCTATATCACATCGCTTGAGATGGCCGGTGTCAGCTTTACGCTTCTCCGCCTGGACGATGAGATGATCAAACTTTGGGAT
GCACCGGTGCATACACCGGCGTTACGTTGGGGCATGTAA

Upstream 100 bases:

>100_bases
GGCGGTTCCATTGCAGCTCTATTGGCGAATGCGTTAGGTATGTGATGTGCTACGCATCGTTGCGAACGGTTAGCAGTCAT
CGCTTCAAGGGAGGAAGCCT

Downstream 100 bases:

>100_bases
GGGCAATTAGCGGTATTGTGGCGGGGTGATTACCCCGCCACCAGATTGGACAAAGAGGGGCCATGCAGATCACCGCCGAT
CATGTCACAAGTTTTCTCCA

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKKLINAPENVVKEALAGMAIAHADLIQVHYDPDYIVRVGAPHNKVGVISGGGSGHEPMHGGFVGYGMLDAACPGAVFTS
PVPDQMLAATKAVNGGKGVLHIVKNYTGDVMNFEMAAELAAAEGIEVASVVTNDDVAVENSTWTAGRRGVGVTVLLEKIV
GAAAEAGADLATCKAIAERVNASGRSMGMALTPCTVPQAGKPGFELADDEMEVGIGIHGEPGRRREKLAPARDIAEMLAG
PILEDLPFKSGDAVLAFVNGMGGTPLIELYVMYNELARILKDRNITIARSLVGSYITSLEMAGVSFTLLRLDDEMIKLWD
APVHTPALRWGM

Sequences:

>Translated_332_residues
MKKLINAPENVVKEALAGMAIAHADLIQVHYDPDYIVRVGAPHNKVGVISGGGSGHEPMHGGFVGYGMLDAACPGAVFTS
PVPDQMLAATKAVNGGKGVLHIVKNYTGDVMNFEMAAELAAAEGIEVASVVTNDDVAVENSTWTAGRRGVGVTVLLEKIV
GAAAEAGADLATCKAIAERVNASGRSMGMALTPCTVPQAGKPGFELADDEMEVGIGIHGEPGRRREKLAPARDIAEMLAG
PILEDLPFKSGDAVLAFVNGMGGTPLIELYVMYNELARILKDRNITIARSLVGSYITSLEMAGVSFTLLRLDDEMIKLWD
APVHTPALRWGM
>Mature_332_residues
MKKLINAPENVVKEALAGMAIAHADLIQVHYDPDYIVRVGAPHNKVGVISGGGSGHEPMHGGFVGYGMLDAACPGAVFTS
PVPDQMLAATKAVNGGKGVLHIVKNYTGDVMNFEMAAELAAAEGIEVASVVTNDDVAVENSTWTAGRRGVGVTVLLEKIV
GAAAEAGADLATCKAIAERVNASGRSMGMALTPCTVPQAGKPGFELADDEMEVGIGIHGEPGRRREKLAPARDIAEMLAG
PILEDLPFKSGDAVLAFVNGMGGTPLIELYVMYNELARILKDRNITIARSLVGSYITSLEMAGVSFTLLRLDDEMIKLWD
APVHTPALRWGM

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=346, Percent_Identity=42.485549132948, Blast_Score=234, Evalue=6e-62,
Organism=Escherichia coli, GI87081857, Length=356, Percent_Identity=50.561797752809, Blast_Score=356, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17565018, Length=327, Percent_Identity=37.0030581039755, Blast_Score=207, Evalue=6e-54,
Organism=Saccharomyces cerevisiae, GI6321055, Length=346, Percent_Identity=38.150289017341, Blast_Score=192, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323570, Length=314, Percent_Identity=37.2611464968153, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 34901; Mature: 34901

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLINAPENVVKEALAGMAIAHADLIQVHYDPDYIVRVGAPHNKVGVISGGGSGHEPMH
CCCCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCEEEEECCCCCCEEEEECCCCCCCCCC
GGFVGYGMLDAACPGAVFTSPVPDQMLAATKAVNGGKGVLHIVKNYTGDVMNFEMAAELA
CCEECCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHEEHHCCCCHHHHHHHHHHH
AAEGIEVASVVTNDDVAVENSTWTAGRRGVGVTVLLEKIVGAAAEAGADLATCKAIAERV
HHCCCEEEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NASGRSMGMALTPCTVPQAGKPGFELADDEMEVGIGIHGEPGRRREKLAPARDIAEMLAG
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHHH
PILEDLPFKSGDAVLAFVNGMGGTPLIELYVMYNELARILKDRNITIARSLVGSYITSLE
HHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MAGVSFTLLRLDDEMIKLWDAPVHTPALRWGM
HCCCEEEEEECCHHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure
MKKLINAPENVVKEALAGMAIAHADLIQVHYDPDYIVRVGAPHNKVGVISGGGSGHEPMH
CCCCCCCCHHHHHHHHHHHHHEEEEEEEEEECCCEEEEECCCCCCEEEEECCCCCCCCCC
GGFVGYGMLDAACPGAVFTSPVPDQMLAATKAVNGGKGVLHIVKNYTGDVMNFEMAAELA
CCEECCCHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHEEHHCCCCHHHHHHHHHHH
AAEGIEVASVVTNDDVAVENSTWTAGRRGVGVTVLLEKIVGAAAEAGADLATCKAIAERV
HHCCCEEEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NASGRSMGMALTPCTVPQAGKPGFELADDEMEVGIGIHGEPGRRREKLAPARDIAEMLAG
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHHH
PILEDLPFKSGDAVLAFVNGMGGTPLIELYVMYNELARILKDRNITIARSLVGSYITSLE
HHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MAGVSFTLLRLDDEMIKLWDAPVHTPALRWGM
HCCCEEEEEECCHHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]