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The map label for this gene is glpF [H]

Identifier: 222526781

GI number: 222526781

Start: 4428601

End: 4429335

Strand: Reverse

Name: glpF [H]

Synonym: Chy400_3555

Alternate gene names: 222526781

Gene position: 4429335-4428601 (Counterclockwise)

Preceding gene: 222526782

Following gene: 222526780

Centisome position: 84.06

GC content: 57.69

Gene sequence:

>735_bases
ATGCCTGCGTCCTTTCTGGGCGAAGTTTTCGGCACAATGGTGCTGATCCTGTTTGGCAATGGTGTTGTTGCCAACGTTCT
GCTGACGCAATCGAAGGGGAATGGTGGTGGCTGGATCGTGATTACCACGGGCTGGGCCTTTGCCGTGATGAGTGGTGTGT
TTACCGCAGCCGCGTTGGGTAGCCCAGGAGCCGATCTCAATCCGGCAGTCACGATTGCCGTGATGATCCGCGGTGGCTAC
GATGTTGGTACTGCGGTGATGCACATTATTGCCCAGTTCATCGGTGCCTTCATTGGGGCAACCCTGGTCTGGCTGACCTA
CCTCTCGCACTGGGAAGTTACCAAAGACCCCGGTCTGAAGCTGGCCTGTTTCAGCACCGGCCCGGCAATCAACAATCCGG
TCAACAACATCATCACCGAAGTGATTGGCACCTTCGCTCTGGTCTTTATCATCTTCGCCATCTTCAGCGGCGATGGCGCT
ACGGGTGGTAGCCCGGCCAGTGGTCTCGGCCCGTACCTGGTTGCGGCACTGGTGTGGGGCATTGGTCTCTCACTCGGTGG
ACCAACCGGCTACGCAATTAACCCGGCCCGCGATCTTGGCCCGCGCATTGCCCACTTTGTGTTGCCTATCGCCGGCAAGG
GCGACAGCAACTGGGGTTACTCGTGGGTACCGGTTGTTGGTCCAATCATTGGCGGTTCCATTGCAGCTCTATTGGCGAAT
GCGTTAGGTATGTGA

Upstream 100 bases:

>100_bases
AAGAATCGGCGCGCATCACGTTCTCTGTTTGAACGCTGGATCAAGGGTGATTCGTAGGAACTGGTTCCGCCTGTTTTGTG
TTGCAAGGAGGAGACCCTCT

Downstream 100 bases:

>100_bases
TGTGCTACGCATCGTTGCGAACGGTTAGCAGTCATCGCTTCAAGGGAGGAAGCCTGTGAAGAAGTTGATCAACGCACCGG
AGAATGTCGTAAAAGAGGCA

Product: major intrinsic protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MPASFLGEVFGTMVLILFGNGVVANVLLTQSKGNGGGWIVITTGWAFAVMSGVFTAAALGSPGADLNPAVTIAVMIRGGY
DVGTAVMHIIAQFIGAFIGATLVWLTYLSHWEVTKDPGLKLACFSTGPAINNPVNNIITEVIGTFALVFIIFAIFSGDGA
TGGSPASGLGPYLVAALVWGIGLSLGGPTGYAINPARDLGPRIAHFVLPIAGKGDSNWGYSWVPVVGPIIGGSIAALLAN
ALGM

Sequences:

>Translated_244_residues
MPASFLGEVFGTMVLILFGNGVVANVLLTQSKGNGGGWIVITTGWAFAVMSGVFTAAALGSPGADLNPAVTIAVMIRGGY
DVGTAVMHIIAQFIGAFIGATLVWLTYLSHWEVTKDPGLKLACFSTGPAINNPVNNIITEVIGTFALVFIIFAIFSGDGA
TGGSPASGLGPYLVAALVWGIGLSLGGPTGYAINPARDLGPRIAHFVLPIAGKGDSNWGYSWVPVVGPIIGGSIAALLAN
ALGM
>Mature_243_residues
PASFLGEVFGTMVLILFGNGVVANVLLTQSKGNGGGWIVITTGWAFAVMSGVFTAAALGSPGADLNPAVTIAVMIRGGYD
VGTAVMHIIAQFIGAFIGATLVWLTYLSHWEVTKDPGLKLACFSTGPAINNPVNNIITEVIGTFALVFIIFAIFSGDGAT
GGSPASGLGPYLVAALVWGIGLSLGGPTGYAINPARDLGPRIAHFVLPIAGKGDSNWGYSWVPVVGPIIGGSIAALLANA
LGM

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=249, Percent_Identity=34.9397590361446, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI157266307, Length=251, Percent_Identity=35.8565737051793, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI22538420, Length=242, Percent_Identity=31.404958677686, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4502187, Length=251, Percent_Identity=30.6772908366534, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1790362, Length=251, Percent_Identity=34.2629482071713, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17544068, Length=249, Percent_Identity=33.7349397590361, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71994009, Length=236, Percent_Identity=30.9322033898305, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17531431, Length=238, Percent_Identity=31.5126050420168, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17531429, Length=238, Percent_Identity=31.5126050420168, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71992966, Length=247, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17533613, Length=251, Percent_Identity=26.6932270916335, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI32564052, Length=251, Percent_Identity=26.6932270916335, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=204, Percent_Identity=34.3137254901961, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322985, Length=280, Percent_Identity=27.5, Blast_Score=76, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24652747, Length=238, Percent_Identity=31.0924369747899, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI45551084, Length=238, Percent_Identity=31.0924369747899, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24774; Mature: 24643

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASFLGEVFGTMVLILFGNGVVANVLLTQSKGNGGGWIVITTGWAFAVMSGVFTAAALG
CCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCC
SPGADLNPAVTIAVMIRGGYDVGTAVMHIIAQFIGAFIGATLVWLTYLSHWEVTKDPGLK
CCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
LACFSTGPAINNPVNNIITEVIGTFALVFIIFAIFSGDGATGGSPASGLGPYLVAALVWG
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
IGLSLGGPTGYAINPARDLGPRIAHFVLPIAGKGDSNWGYSWVPVVGPIIGGSIAALLAN
HHHCCCCCCCCEECCHHHHHHHHHHHHEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
ALGM
HHCC
>Mature Secondary Structure 
PASFLGEVFGTMVLILFGNGVVANVLLTQSKGNGGGWIVITTGWAFAVMSGVFTAAALG
CHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHCC
SPGADLNPAVTIAVMIRGGYDVGTAVMHIIAQFIGAFIGATLVWLTYLSHWEVTKDPGLK
CCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
LACFSTGPAINNPVNNIITEVIGTFALVFIIFAIFSGDGATGGSPASGLGPYLVAALVWG
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
IGLSLGGPTGYAINPARDLGPRIAHFVLPIAGKGDSNWGYSWVPVVGPIIGGSIAALLAN
HHHCCCCCCCCEECCHHHHHHHHHHHHEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
ALGM
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]