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The map label for this gene is purD [H]

Identifier: 222526768

GI number: 222526768

Start: 4411928

End: 4413232

Strand: Direct

Name: purD [H]

Synonym: Chy400_3542

Alternate gene names: 222526768

Gene position: 4411928-4413232 (Clockwise)

Preceding gene: 222526767

Following gene: 222526769

Centisome position: 83.73

GC content: 56.4

Gene sequence:

>1305_bases
ATGAAGATTCTGGTTCTCGGTAATGATGGACGCGCTCACGCGCTGGTTTGGAAACTCTTTGCCAGCCCGACGGCTGACGT
CCTGGTGGCGCCGGGCAACGGTGGTGCGTGCCAGATTGCCCCCCAGGTTGATCTTGATCCACTCCAACCGGCGCATGTAG
CGCAATGGGCATTTAACGAACAGATTGATCTCATCGTTCCTGCCGGAAGTGAACCGCTCTGGGCCGGTTTGGTTGATGAG
GTCGTGAGTATGCATATTGGCGTATGTGGTGCTTCACAGCGCAGCACACGCATCGAATGGAGTCGCTGTTACGCCAAGGA
ATTGCTCTTGCGCTATCAGCTCCCAACACCGAACGGACGCTGTTTTGCTAGCTTACCGATGGCGGAAAAATATCTGGCTG
CTCAGCAATTACCGGTGGTGCTGCGTGGCGATCACCCGGCTACCGGTGAAGGTGTCTACTTCGACCGCTACACAGCCCTT
GAGGCGTTGCGGTCATTCTTTGTTACCCGCCCGGTGGAAGGGAGCAGTCACGGGGTTGTGATTGAAGAATATGTGGCAGG
GCCACTGGTCAGCTTTTCGGCTATTACCGATGGCAGTCATCTGGTATCGCTGTTACCGGCTCGTATCTACGAAGGGATGG
GACCACAGCCAGATAGCCCGCCCGCACCCGGTATGGGGGCAATTACTGGTAATTCAACCTATGCCCAGCGCTTGACGAGC
TATCTTGAACGACACATCATGCAGCCCCTTATCGCGGCAATTGCGCGCGAACAATTGCCCTACTGGGGGATTATCGGCGT
TGATTGCATTATTGGTGATCACGGCCCACGCATCGTTGGCCTGCGTTGCAGTCTTCGCGATATGGAGGCACAGGCTGTTT
TGCCACGCCTGATTGATGATCTGGTGCCATACTACGAAGCCGCCATTGCCCGTAGTTTACAGCGTCTACCGCCACTGCGT
TGGCGCGATGAGGCGAGTGTAGCGCTGGCCCTGGTTACGCAAGGCTACCCTCATCATTATCCGATCAATGCAGCAGTTGA
GGGTATTGGCGATGTGGACGAAGGTGTACTGGTCTTTCACGACCAGACCGAGTCGCCGTTTATCTTGCGCTACGATCCGT
CTCGTCGTGAGCGCCTGTTGGGTAGTCAGAGCGGTAGTCTCTACTTGACCGGTGGTCATGTGGTAACCGTAGTAGCATTG
GGGGCAACGCTGGCCGGTGCACGAGGTCGTGCACTCATTAACGCCGAACGCATTCGCTTTCCCGGTCGGACGTATCGCGA
CGATGTAGGGGCTGGTGAGCGTTGA

Upstream 100 bases:

>100_bases
CGTCAGGTGTTACCAGCCCAAAGGTTGTCTAACTCGTCTCGCTGACTCGTTGGGATATGTGCCACGGAACAGTCCTGGAC
GAGTGAAGGAATGCGCCGGT

Downstream 100 bases:

>100_bases
TCGCACTAAGGGTTCCTGCTTTGATCTATGAGGATAATTGTCGATGAACATCTCGATTGCTGCTACTGATGACGAAATTG
CCGCCTGCTATCCGATCATG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MKILVLGNDGRAHALVWKLFASPTADVLVAPGNGGACQIAPQVDLDPLQPAHVAQWAFNEQIDLIVPAGSEPLWAGLVDE
VVSMHIGVCGASQRSTRIEWSRCYAKELLLRYQLPTPNGRCFASLPMAEKYLAAQQLPVVLRGDHPATGEGVYFDRYTAL
EALRSFFVTRPVEGSSHGVVIEEYVAGPLVSFSAITDGSHLVSLLPARIYEGMGPQPDSPPAPGMGAITGNSTYAQRLTS
YLERHIMQPLIAAIAREQLPYWGIIGVDCIIGDHGPRIVGLRCSLRDMEAQAVLPRLIDDLVPYYEAAIARSLQRLPPLR
WRDEASVALALVTQGYPHHYPINAAVEGIGDVDEGVLVFHDQTESPFILRYDPSRRERLLGSQSGSLYLTGGHVVTVVAL
GATLAGARGRALINAERIRFPGRTYRDDVGAGER

Sequences:

>Translated_434_residues
MKILVLGNDGRAHALVWKLFASPTADVLVAPGNGGACQIAPQVDLDPLQPAHVAQWAFNEQIDLIVPAGSEPLWAGLVDE
VVSMHIGVCGASQRSTRIEWSRCYAKELLLRYQLPTPNGRCFASLPMAEKYLAAQQLPVVLRGDHPATGEGVYFDRYTAL
EALRSFFVTRPVEGSSHGVVIEEYVAGPLVSFSAITDGSHLVSLLPARIYEGMGPQPDSPPAPGMGAITGNSTYAQRLTS
YLERHIMQPLIAAIAREQLPYWGIIGVDCIIGDHGPRIVGLRCSLRDMEAQAVLPRLIDDLVPYYEAAIARSLQRLPPLR
WRDEASVALALVTQGYPHHYPINAAVEGIGDVDEGVLVFHDQTESPFILRYDPSRRERLLGSQSGSLYLTGGHVVTVVAL
GATLAGARGRALINAERIRFPGRTYRDDVGAGER
>Mature_434_residues
MKILVLGNDGRAHALVWKLFASPTADVLVAPGNGGACQIAPQVDLDPLQPAHVAQWAFNEQIDLIVPAGSEPLWAGLVDE
VVSMHIGVCGASQRSTRIEWSRCYAKELLLRYQLPTPNGRCFASLPMAEKYLAAQQLPVVLRGDHPATGEGVYFDRYTAL
EALRSFFVTRPVEGSSHGVVIEEYVAGPLVSFSAITDGSHLVSLLPARIYEGMGPQPDSPPAPGMGAITGNSTYAQRLTS
YLERHIMQPLIAAIAREQLPYWGIIGVDCIIGDHGPRIVGLRCSLRDMEAQAVLPRLIDDLVPYYEAAIARSLQRLPPLR
WRDEASVALALVTQGYPHHYPINAAVEGIGDVDEGVLVFHDQTESPFILRYDPSRRERLLGSQSGSLYLTGGHVVTVVAL
GATLAGARGRALINAERIRFPGRTYRDDVGAGER

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=436, Percent_Identity=25.9174311926606, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI4503915, Length=436, Percent_Identity=25.9174311926606, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI209869995, Length=436, Percent_Identity=25.9174311926606, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI209869993, Length=436, Percent_Identity=25.9174311926606, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1790438, Length=438, Percent_Identity=28.5388127853881, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17567511, Length=438, Percent_Identity=26.2557077625571, Blast_Score=137, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6321203, Length=449, Percent_Identity=26.0579064587973, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI62471619, Length=441, Percent_Identity=27.6643990929705, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24582400, Length=441, Percent_Identity=27.6643990929705, Blast_Score=144, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 47137; Mature: 47137

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVLGNDGRAHALVWKLFASPTADVLVAPGNGGACQIAPQVDLDPLQPAHVAQWAFNE
CEEEEECCCCCCHHHEEHHHCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHCCC
QIDLIVPAGSEPLWAGLVDEVVSMHIGVCGASQRSTRIEWSRCYAKELLLRYQLPTPNGR
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCC
CFASLPMAEKYLAAQQLPVVLRGDHPATGEGVYFDRYTALEALRSFFVTRPVEGSSHGVV
EEEECCHHHHHHHHHHCCEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCEE
IEEYVAGPLVSFSAITDGSHLVSLLPARIYEGMGPQPDSPPAPGMGAITGNSTYAQRLTS
EHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHH
YLERHIMQPLIAAIAREQLPYWGIIGVDCIIGDHGPRIVGLRCSLRDMEAQAVLPRLIDD
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHH
LVPYYEAAIARSLQRLPPLRWRDEASVALALVTQGYPHHYPINAAVEGIGDVDEGVLVFH
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCCCCEEEEE
DQTESPFILRYDPSRRERLLGSQSGSLYLTGGHVVTVVALGATLAGARGRALINAERIRF
CCCCCCEEEEECCCHHHHHHCCCCCCEEEECCCEEEEEEHHHHHCCCCCCEEEEHHHEEC
PGRTYRDDVGAGER
CCCCCCCCCCCCCC
>Mature Secondary Structure
MKILVLGNDGRAHALVWKLFASPTADVLVAPGNGGACQIAPQVDLDPLQPAHVAQWAFNE
CEEEEECCCCCCHHHEEHHHCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHCCC
QIDLIVPAGSEPLWAGLVDEVVSMHIGVCGASQRSTRIEWSRCYAKELLLRYQLPTPNGR
CEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCC
CFASLPMAEKYLAAQQLPVVLRGDHPATGEGVYFDRYTALEALRSFFVTRPVEGSSHGVV
EEEECCHHHHHHHHHHCCEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCEE
IEEYVAGPLVSFSAITDGSHLVSLLPARIYEGMGPQPDSPPAPGMGAITGNSTYAQRLTS
EHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCHHHHHHHHH
YLERHIMQPLIAAIAREQLPYWGIIGVDCIIGDHGPRIVGLRCSLRDMEAQAVLPRLIDD
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHH
LVPYYEAAIARSLQRLPPLRWRDEASVALALVTQGYPHHYPINAAVEGIGDVDEGVLVFH
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCCCCEEEEE
DQTESPFILRYDPSRRERLLGSQSGSLYLTGGHVVTVVALGATLAGARGRALINAERIRF
CCCCCCEEEEECCCHHHHHHCCCCCCEEEECCCEEEEEEHHHHHCCCCCCEEEEHHHEEC
PGRTYRDDVGAGER
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]