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The map label for this gene is rep [C]

Identifier: 222526592

GI number: 222526592

Start: 4187512

End: 4189620

Strand: Reverse

Name: rep [C]

Synonym: Chy400_3360

Alternate gene names: 222526592

Gene position: 4189620-4187512 (Counterclockwise)

Preceding gene: 222526593

Following gene: 222526585

Centisome position: 79.52

GC content: 53.25

Gene sequence:

>2109_bases
ATGACCAAATATCAGCTTATTACACCACCAACCTTTTTCAACGAACTTCTTGGGCTACCGGGCAAAATCCAGAAACTGGT
CACCCAGAAGGTCAAAATCCTTGAGCATGATCCTGTTTCAGCCCAGGGTGATGCCAAAAAGCTCAAGAATCGTGAACCTC
CACTTTATCGAATTAGAATTGGCGACTACCGCCTGATTTATACATTTGGTGCGGGATGGGTCAAGCTGGTTGCTATCCGT
AAACGTGATGATCAGACGTATCAGCGCGATTTTGGTCACGTTGATCTGCCCGATACCGATCTCTCTGAGGCTGTTGCAAT
AGCTTCGGCGAGCGAACCGCTCACTGCGGAGCCTGAACCAACACCACCTCTCCCCCGACAGCTCTCGCCTGATGAAACCA
TAACAGGTAGTCCATCGCAACCACTGCCATACCAGTTGACTGCCGATAATTTACGGCGCTGGTTGATCCCAGAGCAGTAT
TGGGACACACTCATACAGATACAAACCGAAGACGATTTAATCAATGCTCAGGTACCGTCACAGGTCATTGACCGGCTACT
CGATATTTTGTATCCACGGAGCGTTGACGAGATTTTGCAACAACCGCAATACCTGATCGCTGAAGCAGACGATCTCGACC
GGTTTGTTGCCGGTGAACTGACCGATTTTCTGCTGGTTCTTGACCCTGAACAGCAACGGCTGGTCACAGCATCACTCGAA
AGTCCCGTTCTGGTACGGGGTGGACCGGGAACCGGCAAATCAATCGTTGCCCTCTACCGCGTGCAACGCCTTGTTCAGCA
AGGCATGACTCCAGTCTTATTTACAACCTTCACCAACACATTAACCGCCTATTCGGAACAATTACTGGAGCGTCTGCTTG
AAGGTGATCTGACCAGCAACGGGGTAGAAGTCAATACTGTCGATGCGCTAATGTTTCGCCAGTATGCCGCCACCTACGGC
ACACCGCGGATTGCCACTGAAGAGCAGATGATCCAGATGTTGCGTGAGGCACTGCACACCGCGATCCTACCGGCGACAAA
CGAGCACGAACGACTGCGACGGCGTGAGGTGCTACAGACCCTTGGTGAAGCCTATCTGCTCGATGAATTTACCAGTGTGA
TTGAGCATTGGGGCTTGCAGAGTCGTGATGAATACCTGACCAGACCAAGGACAGGCCGTAAGCTTCCGTTACGACCCCCA
ATTCGGGAAGCGATCTGGGCAGTATACGAGACCTGGAAGAGCATGCTCCAGCACCAGCAATTGACGACTTGGGGACAATT
TCGCCAGCGTGCACTCGAACTGGCATTACGGCAATCTCAGCGACCGTATCGCGCCATTGTTATCGACGAAGCACAAGACC
TGTCGCCGGTCGCATTGCGTTACCTGCTGGCCCGTGTTGAGTCGCCCCGTGCGGTCTACCTGACTGCCGACGCCGGTCAG
TCGCTCTATCAACGTGGTTTTTCGTGGCAACAGATCGATAGCGAATTGAATGTTCGTGGTCGCAGCTTTGTTTTGCGGCG
CAACTATCGCAACACAGCCGAGATTGCCACTGCCTGCACATCCATTTTGGGCACGACTGAAAGTGATCAGGAAGTAAGCG
CCGTTCATCACGGCTCTCTGCCGGTAGTGCGTTTTTGTCGTAGTGTTGCAGAAGAAGCCCAGGCGATCCGTAGCTTCTTC
ATTGCTGCCGCTCGCCGCTGGCGACTGCCTGCACACGCCGGTGCAGTGCTTTGCCATAGCAAACAAGTGGCCCAGCAAAT
CTGCGACCAGTTGCAACAACTTGGGATGTCGGCGGAATTTATGGAAAAGCGGCAGATCGACTTGCGAAAGCCGGTTGTCA
AAGTATTGACGATCCACTCAGCAAAGGGATTGGAGTTTCCGTTTGTGGCGGTGATGCGATTAGCGCAGGATCATCTGCCG
CACAACTACGATCATCTACCGGTGGAAGAGCGCGACGCAATGATTGACCAGGAGCGGCGGCTGTTTTACGTCGGCTGTTC
GCGAGCCATGCGTGCTTTACTGGTCTGCGCCGATGCCGATCAGCCATCATCGTTTGTGCTGGAATTGCCGACAACGCTCT
GGCAGCGCGAGGGTGAGATGAGTAGATGA

Upstream 100 bases:

>100_bases
TTCCTTTGCAGGTCTCATTCGAGATGCTATCATAAGAACAGTTTTCAGATCAGGCTTGTTTCTAAGCGTAATCTCTTTGT
CCAACCATCAGGATCGTTCG

Downstream 100 bases:

>100_bases
CAGATAACGTCATTCACCGGCAGAGCTTGACACCTGGAAAGTGAAAGCGGGAACACTGGGCTGACCCGGCGCGTTCCCGC
TCCTGCTGAGTTCGCCAGTG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: DNA Helicase; Helicase; UvrD/Rep Helicase; Plasmid Stabilization System; LexA Repressor; Superfamily I DNA/RNA Helicase-Like Protein; UvrD/REP Helicase Domain Protein; NERD Domain Protein; DNA Helicase II; Superfamily I DNA/RNA Helicase; Superfamily I DNA And RNA Helicase; Superfamily Protein I DNA/RNA Helicase; Nerd Domain Protein; UvrD/REP Type DNA Helicase; UvrD/REP Family ATP-Dependent DNA Helicase; Helicase Protein; NA Helicase.; DNA Helicase II-Like Protein; NERD Domain-Containing Protein; DNA/RNA Helicase Superfamily I; Peptidase; Superfamily I DNA And RNA Helicase-Like Protein; Uvrd/Rep Helicase

Number of amino acids: Translated: 702; Mature: 701

Protein sequence:

>702_residues
MTKYQLITPPTFFNELLGLPGKIQKLVTQKVKILEHDPVSAQGDAKKLKNREPPLYRIRIGDYRLIYTFGAGWVKLVAIR
KRDDQTYQRDFGHVDLPDTDLSEAVAIASASEPLTAEPEPTPPLPRQLSPDETITGSPSQPLPYQLTADNLRRWLIPEQY
WDTLIQIQTEDDLINAQVPSQVIDRLLDILYPRSVDEILQQPQYLIAEADDLDRFVAGELTDFLLVLDPEQQRLVTASLE
SPVLVRGGPGTGKSIVALYRVQRLVQQGMTPVLFTTFTNTLTAYSEQLLERLLEGDLTSNGVEVNTVDALMFRQYAATYG
TPRIATEEQMIQMLREALHTAILPATNEHERLRRREVLQTLGEAYLLDEFTSVIEHWGLQSRDEYLTRPRTGRKLPLRPP
IREAIWAVYETWKSMLQHQQLTTWGQFRQRALELALRQSQRPYRAIVIDEAQDLSPVALRYLLARVESPRAVYLTADAGQ
SLYQRGFSWQQIDSELNVRGRSFVLRRNYRNTAEIATACTSILGTTESDQEVSAVHHGSLPVVRFCRSVAEEAQAIRSFF
IAAARRWRLPAHAGAVLCHSKQVAQQICDQLQQLGMSAEFMEKRQIDLRKPVVKVLTIHSAKGLEFPFVAVMRLAQDHLP
HNYDHLPVEERDAMIDQERRLFYVGCSRAMRALLVCADADQPSSFVLELPTTLWQREGEMSR

Sequences:

>Translated_702_residues
MTKYQLITPPTFFNELLGLPGKIQKLVTQKVKILEHDPVSAQGDAKKLKNREPPLYRIRIGDYRLIYTFGAGWVKLVAIR
KRDDQTYQRDFGHVDLPDTDLSEAVAIASASEPLTAEPEPTPPLPRQLSPDETITGSPSQPLPYQLTADNLRRWLIPEQY
WDTLIQIQTEDDLINAQVPSQVIDRLLDILYPRSVDEILQQPQYLIAEADDLDRFVAGELTDFLLVLDPEQQRLVTASLE
SPVLVRGGPGTGKSIVALYRVQRLVQQGMTPVLFTTFTNTLTAYSEQLLERLLEGDLTSNGVEVNTVDALMFRQYAATYG
TPRIATEEQMIQMLREALHTAILPATNEHERLRRREVLQTLGEAYLLDEFTSVIEHWGLQSRDEYLTRPRTGRKLPLRPP
IREAIWAVYETWKSMLQHQQLTTWGQFRQRALELALRQSQRPYRAIVIDEAQDLSPVALRYLLARVESPRAVYLTADAGQ
SLYQRGFSWQQIDSELNVRGRSFVLRRNYRNTAEIATACTSILGTTESDQEVSAVHHGSLPVVRFCRSVAEEAQAIRSFF
IAAARRWRLPAHAGAVLCHSKQVAQQICDQLQQLGMSAEFMEKRQIDLRKPVVKVLTIHSAKGLEFPFVAVMRLAQDHLP
HNYDHLPVEERDAMIDQERRLFYVGCSRAMRALLVCADADQPSSFVLELPTTLWQREGEMSR
>Mature_701_residues
TKYQLITPPTFFNELLGLPGKIQKLVTQKVKILEHDPVSAQGDAKKLKNREPPLYRIRIGDYRLIYTFGAGWVKLVAIRK
RDDQTYQRDFGHVDLPDTDLSEAVAIASASEPLTAEPEPTPPLPRQLSPDETITGSPSQPLPYQLTADNLRRWLIPEQYW
DTLIQIQTEDDLINAQVPSQVIDRLLDILYPRSVDEILQQPQYLIAEADDLDRFVAGELTDFLLVLDPEQQRLVTASLES
PVLVRGGPGTGKSIVALYRVQRLVQQGMTPVLFTTFTNTLTAYSEQLLERLLEGDLTSNGVEVNTVDALMFRQYAATYGT
PRIATEEQMIQMLREALHTAILPATNEHERLRRREVLQTLGEAYLLDEFTSVIEHWGLQSRDEYLTRPRTGRKLPLRPPI
REAIWAVYETWKSMLQHQQLTTWGQFRQRALELALRQSQRPYRAIVIDEAQDLSPVALRYLLARVESPRAVYLTADAGQS
LYQRGFSWQQIDSELNVRGRSFVLRRNYRNTAEIATACTSILGTTESDQEVSAVHHGSLPVVRFCRSVAEEAQAIRSFFI
AAARRWRLPAHAGAVLCHSKQVAQQICDQLQQLGMSAEFMEKRQIDLRKPVVKVLTIHSAKGLEFPFVAVMRLAQDHLPH
NYDHLPVEERDAMIDQERRLFYVGCSRAMRALLVCADADQPSSFVLELPTTLWQREGEMSR

Specific function: Rep Helicase Is A Single-Stranded DNA-Dependent ATPase Involved In DNA Replication; It Can Initiate Unwinding At A Nick In The DNA. It Binds To The Single-Stranded DNA And Acts In A Progressive Fashion Along The DNA In The 3' To 5' Direction. [C]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994965, Length=111, Percent_Identity=35.1351351351351, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.6.1.- [C]

Molecular weight: Translated: 80027; Mature: 79895

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYQLITPPTFFNELLGLPGKIQKLVTQKVKILEHDPVSAQGDAKKLKNREPPLYRIRI
CCCCEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEEEEE
GDYRLIYTFGAGWVKLVAIRKRDDQTYQRDFGHVDLPDTDLSEAVAIASASEPLTAEPEP
CCEEEEEEECCCCEEEEEECCCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
TPPLPRQLSPDETITGSPSQPLPYQLTADNLRRWLIPEQYWDTLIQIQTEDDLINAQVPS
CCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCHHHHHHHEEEECCCCCCCCCCHH
QVIDRLLDILYPRSVDEILQQPQYLIAEADDLDRFVAGELTDFLLVLDPEQQRLVTASLE
HHHHHHHHHHCCCCHHHHHCCCCEEECCCHHHHHHHHHHHHCEEEEECCCHHHEEEECCC
SPVLVRGGPGTGKSIVALYRVQRLVQQGMTPVLFTTFTNTLTAYSEQLLERLLEGDLTSN
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVEVNTVDALMFRQYAATYGTPRIATEEQMIQMLREALHTAILPATNEHERLRRREVLQT
CEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LGEAYLLDEFTSVIEHWGLQSRDEYLTRPRTGRKLPLRPPIREAIWAVYETWKSMLQHQQ
HHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LTTWGQFRQRALELALRQSQRPYRAIVIDEAQDLSPVALRYLLARVESPRAVYLTADAGQ
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCH
SLYQRGFSWQQIDSELNVRGRSFVLRRNYRNTAEIATACTSILGTTESDQEVSAVHHGSL
HHHHCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
PVVRFCRSVAEEAQAIRSFFIAAARRWRLPAHAGAVLCHSKQVAQQICDQLQQLGMSAEF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHCCHHHHHHHHHHHHHCCCCHHH
MEKRQIDLRKPVVKVLTIHSAKGLEFPFVAVMRLAQDHLPHNYDHLPVEERDAMIDQERR
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCC
LFYVGCSRAMRALLVCADADQPSSFVLELPTTLWQREGEMSR
EEEEEHHHHHHHHHHCCCCCCCCHHEEECCHHHHHCCCCCCC
>Mature Secondary Structure 
TKYQLITPPTFFNELLGLPGKIQKLVTQKVKILEHDPVSAQGDAKKLKNREPPLYRIRI
CCCEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCEEEEEE
GDYRLIYTFGAGWVKLVAIRKRDDQTYQRDFGHVDLPDTDLSEAVAIASASEPLTAEPEP
CCEEEEEEECCCCEEEEEECCCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
TPPLPRQLSPDETITGSPSQPLPYQLTADNLRRWLIPEQYWDTLIQIQTEDDLINAQVPS
CCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCHHHHHHHEEEECCCCCCCCCCHH
QVIDRLLDILYPRSVDEILQQPQYLIAEADDLDRFVAGELTDFLLVLDPEQQRLVTASLE
HHHHHHHHHHCCCCHHHHHCCCCEEECCCHHHHHHHHHHHHCEEEEECCCHHHEEEECCC
SPVLVRGGPGTGKSIVALYRVQRLVQQGMTPVLFTTFTNTLTAYSEQLLERLLEGDLTSN
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCC
GVEVNTVDALMFRQYAATYGTPRIATEEQMIQMLREALHTAILPATNEHERLRRREVLQT
CEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LGEAYLLDEFTSVIEHWGLQSRDEYLTRPRTGRKLPLRPPIREAIWAVYETWKSMLQHQQ
HHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LTTWGQFRQRALELALRQSQRPYRAIVIDEAQDLSPVALRYLLARVESPRAVYLTADAGQ
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCCCH
SLYQRGFSWQQIDSELNVRGRSFVLRRNYRNTAEIATACTSILGTTESDQEVSAVHHGSL
HHHHCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCC
PVVRFCRSVAEEAQAIRSFFIAAARRWRLPAHAGAVLCHSKQVAQQICDQLQQLGMSAEF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHHHCCHHHHHHHHHHHHHCCCCHHH
MEKRQIDLRKPVVKVLTIHSAKGLEFPFVAVMRLAQDHLPHNYDHLPVEERDAMIDQERR
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCC
LFYVGCSRAMRALLVCADADQPSSFVLELPTTLWQREGEMSR
EEEEEHHHHHHHHHHCCCCCCCCHHEEECCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA