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The map label for this gene is glgX [H]

Identifier: 222526587

GI number: 222526587

Start: 4178121

End: 4180283

Strand: Direct

Name: glgX [H]

Synonym: Chy400_3355

Alternate gene names: 222526587

Gene position: 4178121-4180283 (Clockwise)

Preceding gene: 222526586

Following gene: 222526588

Centisome position: 79.3

GC content: 54.92

Gene sequence:

>2163_bases
ATGCATATTACCGACATCTGGCCGGGTCTACCATATCCGCTTGGCGCGACGTGGGACGGTGAGGGGGTAAACTTCGCTAT
CTTCTCAGCCCATGCCACGCGCGTCGAGTTGTGTCTGTTTGATAGTCCAACGGCACCTCGTGAAGCGGTACGTATCGCCT
TGCCAGAACGTACGGATGATGTCTGGCACGGCTACATTCCCGGTTTGCGACCTGGTCAGCTCTACGGTTATCGCGTCCAC
GGCCCGTATCTGCCACAACAGGGGCATCGCTTTAATCCACATAAGCTGTTGATCGATCCTTACGCCCGTGCTCTCTGCGG
CGAATTGACCTGGGATAAGGCAAACTATGGCTACCGCGTTGATAGTCCATACGGTGATCTGACCATCGGTAAGCGGGATA
GCGCACCGTATATCCCACGTTCGGTAGTTATCGATCCCACCTTCGATTGGGGCGATGATCGCCATCCCAATATTCCTTTG
GCCGACTCGGTTATCTACGAGCTGCATGTCAAAGGCTTCACCAGGCTTCACCCAGAAGTACCCGAAAATCTGCGTGGGAC
CTACGCCGGTCTGGCATCGCCGGCAGTTATTGACTATCTCAAGCAATTAGGGGTGACAGCGGTTGAACTCTTGCCGGTCC
ATGCCTTCATTACCGATCAGTTCCTGGCTGATCGTGGTCTGACCAACTATTGGGGGTATCAGCCGATCAACTATTTCAGC
CCGGAGCCGCGCTATGCCAGTCAGTCCGATCCGCAGGCACAGGTGAATGAGTTCAAGGCAATGGTTAAGGCACTCCACGC
CGCCGGGATTGAAGTCATTATCGACGTGGTCTACAACCATACCGGTGAAGGTAATCACCTGGGGCCGACGCTGAGCTTTC
GCGGGATTGACAACGTGGCCTATTACCGCCTCGTTCCCGATCAACCGCGTTACTACCTTGACGACACCGGTACCGGCAAT
AGCCTTGACCTGAACCATCCACGCACGCTTCAGTTAGTGCTCGACTCATTGCGTTATTGGGTCACTGAGATGCATGTTGA
TGGGTTCCGGTTTGATCTTGCCCGTACCCTTGCCCGTGGCCCGGCCGGTTCTGACCTGCCCAGTCCCTTCTTCACGGCTG
TTCGCCAGGACCCGGTGCTCTCGCGGGTGAAACTGATCGCCGAACCGTGGGATGTTGGTTTTGATGGCTATTGGGTAGGT
CGCTTTCCGGCACCCTGGGCAGAATGGAATGGTCGTTACCGCGATACCGTGCGCTGCTTCTGGAAGGGTGATCCCGGTCA
GGCCGCCGATTTTGCCTCGCGTTTTATGGGATCGATGGATCTCTACCATCACAATGGCCGTCGTCCATATCATTCCATCA
ATTTCGTGACGGCGCACGATGGCTTCACCTTGCGCGATCTGGTGTCGTACAACGAAAAGCATAATGAAGCCAACGGTGAA
GATAATCGTGATGGCGATAGCCACAACAACTCGTGGAATTGTGGGGTAGAGGGGCCAACCGATGACCCCGACATTCGGGC
GTTGCGACTGCGACAAATGATGAACTTTATCGCTACGCTCTTTCTGTCACAGGGCACCCCGATGTTACTGGCCGGTGATG
AGCGTGGGCGAACCCAACAAGGAAATAACAACGCCTATTGTCAGGACAACCCGATCTCGTGGGTTGACTGGGAACCCGAC
TCAGAAGCCGAGGTGTTACTTGCGTTTACTCAACGCCTGATCGGCTTTCGTCGTGAACATCCGGTGTTGCGTCGCCGTCG
CTTCTTCGTTGGCCATCTCGGTAACGCCGAATACGATGTTGAGTGGCTCAGTCCTGATGGTCAGGAGGTTAGTCCAGAGT
TGTGGCAACATCCTGAATTGCGTTGTATTGGCGTCTTACTCAATGGTGCGGTTATCGATGATCGCAACGAACGCGGCGAA
TCGATGCGTGATGATGTGTTACTGGTATTGATCAATGCCGGCGATGAGCCGGTACCATTCATTCTGCCCGACTGGCCAGA
CGATGCAAGCTGGCATGTAGTTATCGATACGGCCCGGCCTGACCTTACCACACCGCTACCGGTAACCGATCAAATGTATC
AAATTCAACCGCGGGCACTGGCAGTCTTGATCGAAAAGGTGTCTGTTGCACCGCCGGTACAGATCGAGCCGGCAGCGAAA
TAG

Upstream 100 bases:

>100_bases
TCCGCTCATGATCATTGCTTAACGTCTATTGCAAGGCGGCATGTTGTTGATCGTTGCAGGTCAACACGGCTGCCTTTCTT
ATTGCAGGAGGATTGTGACA

Downstream 100 bases:

>100_bases
CTAATTTGCTTCCTGGTAGCACTTCCGTAAGACCAATTCATATACTAAAATACGTAGTATCCACACTGTGTGCGGCAGGT
ACCCTGCCGCACTTTCCTGG

Product: glycogen debranching protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MHITDIWPGLPYPLGATWDGEGVNFAIFSAHATRVELCLFDSPTAPREAVRIALPERTDDVWHGYIPGLRPGQLYGYRVH
GPYLPQQGHRFNPHKLLIDPYARALCGELTWDKANYGYRVDSPYGDLTIGKRDSAPYIPRSVVIDPTFDWGDDRHPNIPL
ADSVIYELHVKGFTRLHPEVPENLRGTYAGLASPAVIDYLKQLGVTAVELLPVHAFITDQFLADRGLTNYWGYQPINYFS
PEPRYASQSDPQAQVNEFKAMVKALHAAGIEVIIDVVYNHTGEGNHLGPTLSFRGIDNVAYYRLVPDQPRYYLDDTGTGN
SLDLNHPRTLQLVLDSLRYWVTEMHVDGFRFDLARTLARGPAGSDLPSPFFTAVRQDPVLSRVKLIAEPWDVGFDGYWVG
RFPAPWAEWNGRYRDTVRCFWKGDPGQAADFASRFMGSMDLYHHNGRRPYHSINFVTAHDGFTLRDLVSYNEKHNEANGE
DNRDGDSHNNSWNCGVEGPTDDPDIRALRLRQMMNFIATLFLSQGTPMLLAGDERGRTQQGNNNAYCQDNPISWVDWEPD
SEAEVLLAFTQRLIGFRREHPVLRRRRFFVGHLGNAEYDVEWLSPDGQEVSPELWQHPELRCIGVLLNGAVIDDRNERGE
SMRDDVLLVLINAGDEPVPFILPDWPDDASWHVVIDTARPDLTTPLPVTDQMYQIQPRALAVLIEKVSVAPPVQIEPAAK

Sequences:

>Translated_720_residues
MHITDIWPGLPYPLGATWDGEGVNFAIFSAHATRVELCLFDSPTAPREAVRIALPERTDDVWHGYIPGLRPGQLYGYRVH
GPYLPQQGHRFNPHKLLIDPYARALCGELTWDKANYGYRVDSPYGDLTIGKRDSAPYIPRSVVIDPTFDWGDDRHPNIPL
ADSVIYELHVKGFTRLHPEVPENLRGTYAGLASPAVIDYLKQLGVTAVELLPVHAFITDQFLADRGLTNYWGYQPINYFS
PEPRYASQSDPQAQVNEFKAMVKALHAAGIEVIIDVVYNHTGEGNHLGPTLSFRGIDNVAYYRLVPDQPRYYLDDTGTGN
SLDLNHPRTLQLVLDSLRYWVTEMHVDGFRFDLARTLARGPAGSDLPSPFFTAVRQDPVLSRVKLIAEPWDVGFDGYWVG
RFPAPWAEWNGRYRDTVRCFWKGDPGQAADFASRFMGSMDLYHHNGRRPYHSINFVTAHDGFTLRDLVSYNEKHNEANGE
DNRDGDSHNNSWNCGVEGPTDDPDIRALRLRQMMNFIATLFLSQGTPMLLAGDERGRTQQGNNNAYCQDNPISWVDWEPD
SEAEVLLAFTQRLIGFRREHPVLRRRRFFVGHLGNAEYDVEWLSPDGQEVSPELWQHPELRCIGVLLNGAVIDDRNERGE
SMRDDVLLVLINAGDEPVPFILPDWPDDASWHVVIDTARPDLTTPLPVTDQMYQIQPRALAVLIEKVSVAPPVQIEPAAK
>Mature_720_residues
MHITDIWPGLPYPLGATWDGEGVNFAIFSAHATRVELCLFDSPTAPREAVRIALPERTDDVWHGYIPGLRPGQLYGYRVH
GPYLPQQGHRFNPHKLLIDPYARALCGELTWDKANYGYRVDSPYGDLTIGKRDSAPYIPRSVVIDPTFDWGDDRHPNIPL
ADSVIYELHVKGFTRLHPEVPENLRGTYAGLASPAVIDYLKQLGVTAVELLPVHAFITDQFLADRGLTNYWGYQPINYFS
PEPRYASQSDPQAQVNEFKAMVKALHAAGIEVIIDVVYNHTGEGNHLGPTLSFRGIDNVAYYRLVPDQPRYYLDDTGTGN
SLDLNHPRTLQLVLDSLRYWVTEMHVDGFRFDLARTLARGPAGSDLPSPFFTAVRQDPVLSRVKLIAEPWDVGFDGYWVG
RFPAPWAEWNGRYRDTVRCFWKGDPGQAADFASRFMGSMDLYHHNGRRPYHSINFVTAHDGFTLRDLVSYNEKHNEANGE
DNRDGDSHNNSWNCGVEGPTDDPDIRALRLRQMMNFIATLFLSQGTPMLLAGDERGRTQQGNNNAYCQDNPISWVDWEPD
SEAEVLLAFTQRLIGFRREHPVLRRRRFFVGHLGNAEYDVEWLSPDGQEVSPELWQHPELRCIGVLLNGAVIDDRNERGE
SMRDDVLLVLINAGDEPVPFILPDWPDDASWHVVIDTARPDLTTPLPVTDQMYQIQPRALAVLIEKVSVAPPVQIEPAAK

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=701, Percent_Identity=46.2196861626248, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI1789839, Length=357, Percent_Identity=25.2100840336134, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 81451; Mature: 81451

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHITDIWPGLPYPLGATWDGEGVNFAIFSAHATRVELCLFDSPTAPREAVRIALPERTDD
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHEEEECCCCCCC
VWHGYIPGLRPGQLYGYRVHGPYLPQQGHRFNPHKLLIDPYARALCGELTWDKANYGYRV
HHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHCCCCCCCCCCCEEE
DSPYGDLTIGKRDSAPYIPRSVVIDPTFDWGDDRHPNIPLADSVIYELHVKGFTRLHPEV
CCCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHEEEEEEECCHHHCCCCC
PENLRGTYAGLASPAVIDYLKQLGVTAVELLPVHAFITDQFLADRGLTNYWGYQPINYFS
CHHHCCCHHCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PEPRYASQSDPQAQVNEFKAMVKALHAAGIEVIIDVVYNHTGEGNHLGPTLSFRGIDNVA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHEEEHHHCCCCCCCCCCCEEEECCCCCEE
YYRLVPDQPRYYLDDTGTGNSLDLNHPRTLQLVLDSLRYWVTEMHVDGFRFDLARTLARG
EEEECCCCCCEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
PAGSDLPSPFFTAVRQDPVLSRVKLIAEPWDVGFDGYWVGRFPAPWAEWNGRYRDTVRCF
CCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHCCCCEEEEEEEE
WKGDPGQAADFASRFMGSMDLYHHNGRRPYHSINFVTAHDGFTLRDLVSYNEKHNEANGE
ECCCCCCHHHHHHHHHCCHHHEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCC
DNRDGDSHNNSWNCGVEGPTDDPDIRALRLRQMMNFIATLFLSQGTPMLLAGDERGRTQQ
CCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
GNNNAYCQDNPISWVDWEPDSEAEVLLAFTQRLIGFRREHPVLRRRRFFVGHLGNAEYDV
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHEEECCCCEEEE
EWLSPDGQEVSPELWQHPELRCIGVLLNGAVIDDRNERGESMRDDVLLVLINAGDEPVPF
EEECCCCCCCCHHHHCCCCCEEEEEEECCEEEECCCCCCCCCCCCEEEEEECCCCCCCCE
ILPDWPDDASWHVVIDTARPDLTTPLPVTDQMYQIQPRALAVLIEKVSVAPPVQIEPAAK
ECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCEECCCCC
>Mature Secondary Structure
MHITDIWPGLPYPLGATWDGEGVNFAIFSAHATRVELCLFDSPTAPREAVRIALPERTDD
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHEEEECCCCCCC
VWHGYIPGLRPGQLYGYRVHGPYLPQQGHRFNPHKLLIDPYARALCGELTWDKANYGYRV
HHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHCCCCCCCCCCCEEE
DSPYGDLTIGKRDSAPYIPRSVVIDPTFDWGDDRHPNIPLADSVIYELHVKGFTRLHPEV
CCCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHEEEEEEECCHHHCCCCC
PENLRGTYAGLASPAVIDYLKQLGVTAVELLPVHAFITDQFLADRGLTNYWGYQPINYFS
CHHHCCCHHCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PEPRYASQSDPQAQVNEFKAMVKALHAAGIEVIIDVVYNHTGEGNHLGPTLSFRGIDNVA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHEEEHHHCCCCCCCCCCCEEEECCCCCEE
YYRLVPDQPRYYLDDTGTGNSLDLNHPRTLQLVLDSLRYWVTEMHVDGFRFDLARTLARG
EEEECCCCCCEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
PAGSDLPSPFFTAVRQDPVLSRVKLIAEPWDVGFDGYWVGRFPAPWAEWNGRYRDTVRCF
CCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHCCCCEEEEEEEE
WKGDPGQAADFASRFMGSMDLYHHNGRRPYHSINFVTAHDGFTLRDLVSYNEKHNEANGE
ECCCCCCHHHHHHHHHCCHHHEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCC
DNRDGDSHNNSWNCGVEGPTDDPDIRALRLRQMMNFIATLFLSQGTPMLLAGDERGRTQQ
CCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
GNNNAYCQDNPISWVDWEPDSEAEVLLAFTQRLIGFRREHPVLRRRRFFVGHLGNAEYDV
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHEEECCCCEEEE
EWLSPDGQEVSPELWQHPELRCIGVLLNGAVIDDRNERGESMRDDVLLVLINAGDEPVPF
EEECCCCCCCCHHHHCCCCCEEEEEEECCEEEECCCCCCCCCCCCEEEEEECCCCCCCCE
ILPDWPDDASWHVVIDTARPDLTTPLPVTDQMYQIQPRALAVLIEKVSVAPPVQIEPAAK
ECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]