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The map label for this gene is def

Identifier: 222526574

GI number: 222526574

Start: 4164119

End: 4164685

Strand: Reverse

Name: def

Synonym: Chy400_3342

Alternate gene names: 222526574

Gene position: 4164685-4164119 (Counterclockwise)

Preceding gene: 222526577

Following gene: 222526573

Centisome position: 79.04

GC content: 55.38

Gene sequence:

>567_bases
ATGGCTATTCGACGTATTTTGCGGATTGATGATGCTGAGGATCGCAAGATCCTCAAGATGCAATGTCGCCCGGTCAAGTT
GCCAGATCGCAATCTCAAGCAACTGGTGGCCGACATGTTTGAAACCATGCGCGCTGCTCACGGAGTCGGGCTGGCCGCGC
CCCAAATTGGCATTCCCATCCAGCTTTGTATTATTGAAATCCCTGCCGAATATGAGGAGCGTGCCGACGGCAGTGTGGTT
GAAGTGGCACCAGCCGAACCATACGTGTTGATTAACCCGCGCATTGTCAAGATGAGCGGGGAAGAGGTCATGCGCGACGA
AGGGTGTCTCAGTCTGCCCGGCTGGTACGGGATGGTGCCGCGTCAGACCTGGGTAACAGTTGAGTTTCAGGATTTGAACG
GCAAACACCATCGCTTGCGCCGCGCCGGTGATCTATTGGGCTGGGCCATTCAGCACGAAGTTGATCATCTCAACGGGATT
CTCTTCACGGAACGGATCCGTGATCTCAGCACGTTGCGCGATATTACGAAGGAGCGTGACGCACAACCGGTTGACCAGGC
TCCGTAG

Upstream 100 bases:

>100_bases
TCTTTCATCCCACCATCTGGAAACCTTGCCGCAAACCTCACGCTGTCGTAGAATGGCAGAAGTAACTGATTGAAGCCTAC
GGGCAAGGAACACACATCAT

Downstream 100 bases:

>100_bases
CCATGGTAGTGCGTCACGTGACATCTGGCCTGATCTGAACCGGTATGCAAACACCTGATCTGGTACAGGCTGCACTCTTC
TTTGCTGCCCAGGCTCACAA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MAIRRILRIDDAEDRKILKMQCRPVKLPDRNLKQLVADMFETMRAAHGVGLAAPQIGIPIQLCIIEIPAEYEERADGSVV
EVAPAEPYVLINPRIVKMSGEEVMRDEGCLSLPGWYGMVPRQTWVTVEFQDLNGKHHRLRRAGDLLGWAIQHEVDHLNGI
LFTERIRDLSTLRDITKERDAQPVDQAP

Sequences:

>Translated_188_residues
MAIRRILRIDDAEDRKILKMQCRPVKLPDRNLKQLVADMFETMRAAHGVGLAAPQIGIPIQLCIIEIPAEYEERADGSVV
EVAPAEPYVLINPRIVKMSGEEVMRDEGCLSLPGWYGMVPRQTWVTVEFQDLNGKHHRLRRAGDLLGWAIQHEVDHLNGI
LFTERIRDLSTLRDITKERDAQPVDQAP
>Mature_187_residues
AIRRILRIDDAEDRKILKMQCRPVKLPDRNLKQLVADMFETMRAAHGVGLAAPQIGIPIQLCIIEIPAEYEERADGSVVE
VAPAEPYVLINPRIVKMSGEEVMRDEGCLSLPGWYGMVPRQTWVTVEFQDLNGKHHRLRRAGDLLGWAIQHEVDHLNGIL
FTERIRDLSTLRDITKERDAQPVDQAP

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=150, Percent_Identity=32, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1789682, Length=173, Percent_Identity=36.9942196531792, Blast_Score=97, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24645728, Length=168, Percent_Identity=32.7380952380952, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_CHLAA (A9WHG7)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001636682.1
- ProteinModelPortal:   A9WHG7
- SMR:   A9WHG7
- GeneID:   5827568
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_3094
- HOGENOM:   HBG665227
- OMA:   IIMHEND
- ProtClustDB:   CLSK973314
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 21394; Mature: 21263

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: ACT_SITE 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRRILRIDDAEDRKILKMQCRPVKLPDRNLKQLVADMFETMRAAHGVGLAAPQIGIPI
CCCHHEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
QLCIIEIPAEYEERADGSVVEVAPAEPYVLINPRIVKMSGEEVMRDEGCLSLPGWYGMVP
EEEEEECCCHHHHCCCCCEEEECCCCCEEEECCEEEEECCHHHHHCCCCEECCCCCCCCC
RQTWVTVEFQDLNGKHHRLRRAGDLLGWAIQHEVDHLNGILFTERIRDLSTLRDITKERD
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
AQPVDQAP
CCCCCCCC
>Mature Secondary Structure 
AIRRILRIDDAEDRKILKMQCRPVKLPDRNLKQLVADMFETMRAAHGVGLAAPQIGIPI
CCHHEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
QLCIIEIPAEYEERADGSVVEVAPAEPYVLINPRIVKMSGEEVMRDEGCLSLPGWYGMVP
EEEEEECCCHHHHCCCCCEEEECCCCCEEEECCEEEEECCHHHHHCCCCEECCCCCCCCC
RQTWVTVEFQDLNGKHHRLRRAGDLLGWAIQHEVDHLNGILFTERIRDLSTLRDITKERD
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
AQPVDQAP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA