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The map label for this gene is nusA [H]

Identifier: 222526490

GI number: 222526490

Start: 4062836

End: 4064164

Strand: Direct

Name: nusA [H]

Synonym: Chy400_3257

Alternate gene names: 222526490

Gene position: 4062836-4064164 (Clockwise)

Preceding gene: 222526489

Following gene: 222526491

Centisome position: 77.11

GC content: 54.63

Gene sequence:

>1329_bases
ATGAAAAGCGATTTTTACGCGGCAATTACTCAGATTGCATCCGAGCGCGGTATTCCAAAAGAGGCCATCATCGATGTGAT
GGAACGGGCATTGGTGAGTGCGTATCGACGTTTATTAGGCCCGAATCCGCCGGCTATCGATGTGGTTGTCAAGCTCGATC
CGGTAAGTGGTGTGGCGCGGGTGTACGCTGAGAAACAGGTTGTTGATGAAGTGTACGATGAGCGTTTTGAGATCGATCTC
GAAGCGGCTCGCCGCATTAAACCTGATGCGCAGATCGGCGAGGCAGTCCTGGTCGAGAGTACCCCGCGTGATTTCGGGCG
GATTGCTGCTCAAACCGCCAAGCAAGTTGTCTTGCAGGGCATCAAGGATATTGAGCGCAATTACATCTACAGCGAGTTTG
AGGATCGCGAAGGGGAATTGCTCACGGCAACCGTTCAGCGCAATAACGGCCCGCGCGGTAACGTGATTCTTGAACTTGGG
AAAGCCGAAGCCATCATTCCACCGAAAGAGCAGGTAGCGAATGACCGCTACTACCACGGGCAGCGGCTGAAAGTCTATTT
GATGGAAGTCAAGAAGGATGAGCGAGGGCCACGCCTGATCGCCTCTCGTGCGCATAAGAATTTGATCCTCCGTCTCTTCG
AGATGGAGGTGCCTGAAATCTACAATGGCGCCGTTGAGATTAAGTCTATCGCTCGCGAGCCGGGGCTGCGCACGAAGGTC
GCCGTCGCCGCTCGTCAAGAGGGGATCGATCCGGTTGGTTCGTGTGTCGGTATGCGCGGTATCCGCATTCAGAATATCGT
GAACGAGCTGAACGGCGAGAAGATCGATGTTGTGCAGTGGTCGTCTGATCCGCGCGAGTACATTGCCAACGCACTTTCGC
CGGCTCAGGTGGTCGAAGTTCATCTTAATGATCACGAGCACACCGCCCTGGTTATTGTGCCCGATAAGCAATTGTCACTG
GCAATTGGTAAAGAAGGGCAAAATGTACGCCTGGCCGCGAAGCTGACCGGTTGGCGAATCGATATCAAGAGTGCCTCGGT
GCTGCTTGAAGAGGAACGAGCAGCGGCTGAGGCGCGTGCGGAGGCGGAAGCTGAACAGATGGCGATTGAGGCTGAGCTGG
CAATGGCTCCGATAGAGACACGAGTGGTGCAACCGGATGGTACCATTCGCTACCGTGGCCATCGCTATGGCCCTCTTGGT
TCCGATCTGGTCGGTAAGACGGTACAGGTGCGAGCGACCTCGCAGAAGATTTTTATCTATTACAATGACCGCCTGTTCGC
TTCCTACATTTTGCTGGAAGGTGGACAGGTAGTGCCCGATGAGGAGTAA

Upstream 100 bases:

>100_bases
CTTCCGCTGCCTCTGTGTAGTGGCTTATCGCAGCAGTCTGCGTTGTTCGGGTGGAAAGTGGGTTGAACCCACTTTTCTTA
TTATCAGGCGAGTTACAGCG

Downstream 100 bases:

>100_bases
GGTCATGGCGGCCAACCCTAAGACCGCTACACCACGTCCACGGCACGTACCGAAGCGTATGTGCATTGTTTGTCGCCAGA
GTGATGCCAAACGTGCATTG

Product: NusA antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKSDFYAAITQIASERGIPKEAIIDVMERALVSAYRRLLGPNPPAIDVVVKLDPVSGVARVYAEKQVVDEVYDERFEIDL
EAARRIKPDAQIGEAVLVESTPRDFGRIAAQTAKQVVLQGIKDIERNYIYSEFEDREGELLTATVQRNNGPRGNVILELG
KAEAIIPPKEQVANDRYYHGQRLKVYLMEVKKDERGPRLIASRAHKNLILRLFEMEVPEIYNGAVEIKSIAREPGLRTKV
AVAARQEGIDPVGSCVGMRGIRIQNIVNELNGEKIDVVQWSSDPREYIANALSPAQVVEVHLNDHEHTALVIVPDKQLSL
AIGKEGQNVRLAAKLTGWRIDIKSASVLLEEERAAAEARAEAEAEQMAIEAELAMAPIETRVVQPDGTIRYRGHRYGPLG
SDLVGKTVQVRATSQKIFIYYNDRLFASYILLEGGQVVPDEE

Sequences:

>Translated_442_residues
MKSDFYAAITQIASERGIPKEAIIDVMERALVSAYRRLLGPNPPAIDVVVKLDPVSGVARVYAEKQVVDEVYDERFEIDL
EAARRIKPDAQIGEAVLVESTPRDFGRIAAQTAKQVVLQGIKDIERNYIYSEFEDREGELLTATVQRNNGPRGNVILELG
KAEAIIPPKEQVANDRYYHGQRLKVYLMEVKKDERGPRLIASRAHKNLILRLFEMEVPEIYNGAVEIKSIAREPGLRTKV
AVAARQEGIDPVGSCVGMRGIRIQNIVNELNGEKIDVVQWSSDPREYIANALSPAQVVEVHLNDHEHTALVIVPDKQLSL
AIGKEGQNVRLAAKLTGWRIDIKSASVLLEEERAAAEARAEAEAEQMAIEAELAMAPIETRVVQPDGTIRYRGHRYGPLG
SDLVGKTVQVRATSQKIFIYYNDRLFASYILLEGGQVVPDEE
>Mature_442_residues
MKSDFYAAITQIASERGIPKEAIIDVMERALVSAYRRLLGPNPPAIDVVVKLDPVSGVARVYAEKQVVDEVYDERFEIDL
EAARRIKPDAQIGEAVLVESTPRDFGRIAAQTAKQVVLQGIKDIERNYIYSEFEDREGELLTATVQRNNGPRGNVILELG
KAEAIIPPKEQVANDRYYHGQRLKVYLMEVKKDERGPRLIASRAHKNLILRLFEMEVPEIYNGAVEIKSIAREPGLRTKV
AVAARQEGIDPVGSCVGMRGIRIQNIVNELNGEKIDVVQWSSDPREYIANALSPAQVVEVHLNDHEHTALVIVPDKQLSL
AIGKEGQNVRLAAKLTGWRIDIKSASVLLEEERAAAEARAEAEAEQMAIEAELAMAPIETRVVQPDGTIRYRGHRYGPLG
SDLVGKTVQVRATSQKIFIYYNDRLFASYILLEGGQVVPDEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=380, Percent_Identity=35.7894736842105, Blast_Score=246, Evalue=3e-66,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 49234; Mature: 49234

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDFYAAITQIASERGIPKEAIIDVMERALVSAYRRLLGPNPPAIDVVVKLDPVSGVAR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHH
VYAEKQVVDEVYDERFEIDLEAARRIKPDAQIGEAVLVESTPRDFGRIAAQTAKQVVLQG
HHHHHHHHHHHHHHHEEEEHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
IKDIERNYIYSEFEDREGELLTATVQRNNGPRGNVILELGKAEAIIPPKEQVANDRYYHG
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHCCCCEECC
QRLKVYLMEVKKDERGPRLIASRAHKNLILRLFEMEVPEIYNGAVEIKSIAREPGLRTKV
CEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCCCCHHH
AVAARQEGIDPVGSCVGMRGIRIQNIVNELNGEKIDVVQWSSDPREYIANALSPAQVVEV
HHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCCCEEEEE
HLNDHEHTALVIVPDKQLSLAIGKEGQNVRLAAKLTGWRIDIKSASVLLEEERAAAEARA
EECCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEECEEEEECHHHEEHHHHHHHHHHHH
EAEAEQMAIEAELAMAPIETRVVQPDGTIRYRGHRYGPLGSDLVGKTVQVRATSQKIFIY
HHHHHHHHHHHHHHCCCCHHEEECCCCCEEECCCCCCCCCCHHCCCEEEEEEECEEEEEE
YNDRLFASYILLEGGQVVPDEE
ECCCEEEEEEEEECCEECCCCC
>Mature Secondary Structure
MKSDFYAAITQIASERGIPKEAIIDVMERALVSAYRRLLGPNPPAIDVVVKLDPVSGVAR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHH
VYAEKQVVDEVYDERFEIDLEAARRIKPDAQIGEAVLVESTPRDFGRIAAQTAKQVVLQG
HHHHHHHHHHHHHHHEEEEHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
IKDIERNYIYSEFEDREGELLTATVQRNNGPRGNVILELGKAEAIIPPKEQVANDRYYHG
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHCCCCEECC
QRLKVYLMEVKKDERGPRLIASRAHKNLILRLFEMEVPEIYNGAVEIKSIAREPGLRTKV
CEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCCCCHHH
AVAARQEGIDPVGSCVGMRGIRIQNIVNELNGEKIDVVQWSSDPREYIANALSPAQVVEV
HHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCCCEEEEE
HLNDHEHTALVIVPDKQLSLAIGKEGQNVRLAAKLTGWRIDIKSASVLLEEERAAAEARA
EECCCCCEEEEEECCCCEEEEECCCCCCEEEEEEEECEEEEECHHHEEHHHHHHHHHHHH
EAEAEQMAIEAELAMAPIETRVVQPDGTIRYRGHRYGPLGSDLVGKTVQVRATSQKIFIY
HHHHHHHHHHHHHHCCCCHHEEECCCCCEEECCCCCCCCCCHHCCCEEEEEEECEEEEEE
YNDRLFASYILLEGGQVVPDEE
ECCCEEEEEEEEECCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]