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The map label for this gene is nuoE2 [H]

Identifier: 222526372

GI number: 222526372

Start: 3881061

End: 3881753

Strand: Direct

Name: nuoE2 [H]

Synonym: Chy400_3138

Alternate gene names: 222526372

Gene position: 3881061-3881753 (Clockwise)

Preceding gene: 222526371

Following gene: 222526373

Centisome position: 73.66

GC content: 56.71

Gene sequence:

>693_bases
GTGACTCTGCTCGAAACACATCAGGCTGAGATTGAGGCAATCGTGGCGCGCTACGCCAGTAAGCGTAGTGCAGTGTTGCC
GTTGCTGTATCTCGCTCAGGATACCTACGGCTATCTGACCGATGAGGCAATTCGGGAAGTGGCGACCATCCTTGATCTGC
CGCCAACCGATGTCTACGAGGTGGTTGGCTTTTATACCCTCTTCTATGATCGCCCGGTGGGTACCTGGGTGTTGCAGGTC
TGTGATGACGTTCCCTGTTGTTTCTGTGGTGCCGAGGAGTTGATTTCCGCCTTGAAACAGGCGCTCGGCATTCGTGAGGA
AGAGACGACTGCCGATGGGATGTTTACCCTCCAGCGCGTCAAGTGTCTGGCGGCATGTGATCGGGCACCGGTGTTGCAGG
CCAATCTTGATTATGTGTACGATGTCACTCCCGAACGGGTCGAAGTGTTGCTCAACAACCTGCGCGCACGTGCCGCCGAA
GCACGCAAGCGGGGGGTGAGTGGTCGTTTCGCTGAAGATTATGAATTTACCCGTGATGGTCGTCTGGTTCAGATTGAACG
GTTTTCACCTTACCGGCCACGTGAGTTTGTGACCGAACCACCGGTCACTGAGGTTACGCCACCGCCGGCGGTGGTACCAG
AGGCTGAGTCTGGTATTGATCGCCCGGAAACCCGGAAGGCCTCGCCGCTGTGA

Upstream 100 bases:

>100_bases
AGGGCCGGATGGTTGCCGATCTGGTCGCGTTAATTGCCAGCCTCGATCCGGTGTTGGGCGAGGTTGATCGCTAACCGACT
ATCATACTGCGGAGGAAAAC

Downstream 100 bases:

>100_bases
TGATGCGCCGGGATGTTCGCCATTTGGTGCGCTAGAAAGTTGGGTTCGATGGGGCTGAGTGAACATATTGTGATGCGGGA
ACTCGATATTCCCGACATTT

Product: NADH-quinone oxidoreductase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTLLETHQAEIEAIVARYASKRSAVLPLLYLAQDTYGYLTDEAIREVATILDLPPTDVYEVVGFYTLFYDRPVGTWVLQV
CDDVPCCFCGAEELISALKQALGIREEETTADGMFTLQRVKCLAACDRAPVLQANLDYVYDVTPERVEVLLNNLRARAAE
ARKRGVSGRFAEDYEFTRDGRLVQIERFSPYRPREFVTEPPVTEVTPPPAVVPEAESGIDRPETRKASPL

Sequences:

>Translated_230_residues
MTLLETHQAEIEAIVARYASKRSAVLPLLYLAQDTYGYLTDEAIREVATILDLPPTDVYEVVGFYTLFYDRPVGTWVLQV
CDDVPCCFCGAEELISALKQALGIREEETTADGMFTLQRVKCLAACDRAPVLQANLDYVYDVTPERVEVLLNNLRARAAE
ARKRGVSGRFAEDYEFTRDGRLVQIERFSPYRPREFVTEPPVTEVTPPPAVVPEAESGIDRPETRKASPL
>Mature_229_residues
TLLETHQAEIEAIVARYASKRSAVLPLLYLAQDTYGYLTDEAIREVATILDLPPTDVYEVVGFYTLFYDRPVGTWVLQVC
DDVPCCFCGAEELISALKQALGIREEETTADGMFTLQRVKCLAACDRAPVLQANLDYVYDVTPERVEVLLNNLRARAAEA
RKRGVSGRFAEDYEFTRDGRLVQIERFSPYRPREFVTEPPVTEVTPPPAVVPEAESGIDRPETRKASPL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=164, Percent_Identity=42.6829268292683, Blast_Score=129, Evalue=2e-30,
Organism=Escherichia coli, GI1788621, Length=145, Percent_Identity=34.4827586206897, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17561328, Length=170, Percent_Identity=45.2941176470588, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI18859877, Length=170, Percent_Identity=40.5882352941176, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21355353, Length=170, Percent_Identity=35.2941176470588, Blast_Score=107, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 25824; Mature: 25692

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLETHQAEIEAIVARYASKRSAVLPLLYLAQDTYGYLTDEAIREVATILDLPPTDVYE
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
VVGFYTLFYDRPVGTWVLQVCDDVPCCFCGAEELISALKQALGIREEETTADGMFTLQRV
HHHHHHHHHCCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
KCLAACDRAPVLQANLDYVYDVTPERVEVLLNNLRARAAEARKRGVSGRFAEDYEFTRDG
HHHHHHCCCCEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
RLVQIERFSPYRPREFVTEPPVTEVTPPPAVVPEAESGIDRPETRKASPL
CEEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLLETHQAEIEAIVARYASKRSAVLPLLYLAQDTYGYLTDEAIREVATILDLPPTDVYE
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
VVGFYTLFYDRPVGTWVLQVCDDVPCCFCGAEELISALKQALGIREEETTADGMFTLQRV
HHHHHHHHHCCCHHHHHHHHHCCCCEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
KCLAACDRAPVLQANLDYVYDVTPERVEVLLNNLRARAAEARKRGVSGRFAEDYEFTRDG
HHHHHHCCCCEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
RLVQIERFSPYRPREFVTEPPVTEVTPPPAVVPEAESGIDRPETRKASPL
CEEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]