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The map label for this gene is nuoF2 [H]

Identifier: 222526373

GI number: 222526373

Start: 3881802

End: 3883106

Strand: Direct

Name: nuoF2 [H]

Synonym: Chy400_3139

Alternate gene names: 222526373

Gene position: 3881802-3883106 (Clockwise)

Preceding gene: 222526372

Following gene: 222526374

Centisome position: 73.67

GC content: 56.17

Gene sequence:

>1305_bases
ATGGGGCTGAGTGAACATATTGTGATGCGGGAACTCGATATTCCCGACATTTCCGACTTTGCCGTTTATCGCCGTCATGG
TGGGTGGGAAGCTTATCGCTACGCTCTGACCGAGAAGACTCCAGCCGATATTGTACAGATGGTGAAGGACTCCGGTCTGC
GTGGTCGCGGTGGGGCCGGCTTTCCCACCGGGATAAAGTGGGGTTTCCTGCCCAAAGGTGTCTACCCGCGTTATCTGCTC
TGCAATTGCGATGAGTCTGAACCAGGGACGTTCAACAATCATCAGATTATTGATAAAAATCCCCATCAATTAATTGAAGG
GGTAGCATTGTCGGCCTATGCGATTGAGGCTAACACTGCCTATATCTACATGCGCGGTGAATTTGCCGCTGCTGCCCGCA
CCCTGGAACGGGCCATTGCCCAGGCCTACGAAGCCGGTTTTCTAGGCAAAAATATCTTTGGCACGGGGTTTGATCTCGAT
ATTTACGTGCATCGCGGTGCCGGCGCCTACATCTGTGGCGAAGAGACGGCTCTGATGGAGTCGCTGGAGGGCAAGATTGG
TCAGCCGCGTCTGAAACCGCCCTTCCCAGCGGTTGCCGGTCTGTATGGCAAACCGACGATTATCAACAATGTTGAGACGC
TGGCCAATGTGCCGCGGATCGTCGAGAAGGGGGTGGCCTGGTACCGTTCGCATGGCACCGAAAAGAGTCCCGGTACCAAG
TGCTTCTCACTCAGCGGCCATGTCAACCGCCCGGGAAACTATGAATTACCGTTTGGCGTTCCGCTCCGCGAGCTGATCGA
GTCGCCGGAGTACGGCGGTGGGATGAAGGGCGGCCGTCCGGTAAAGATTATCATTCCCGGTGGTGCTTCTGCGCCATGGT
TGACCGCCGATCATCTCGATGTGCCGCTCGATTACGAAAGTGTCGCGGCTGCCGGTTCGATGCTCGGTTCAGGTGCTGTG
ATCGTCCTGAACGATACCGTTTCGGCACCGCATGTCGCGTATAAAATGGACGAGTTCTTCAAGCACGAGAGCTGCGGCAA
GTGTACGCCGTGCCGTGAAGGTACCCATTGGCTGGTCCGCACGCTCCACCGTATGGTCGAAGAGGGGCATGCCACCGCTG
CCGATATTGATACCCTGCACAGCATTTATCGCCAGATGGCCGGTAATTGCTTCTGCCTGCTCGGCGAGAGTGCAGTTATG
CCGATCAAGAGTGCATTGCAGCTCTTCCCCCACGAGTTTGAAGCACTGGTCAAGCGGAGTAGCAGCCGTAACGGTCATCA
TATTCCGCTCGCCGTCCATCGCTAG

Upstream 100 bases:

>100_bases
GGCTGAGTCTGGTATTGATCGCCCGGAAACCCGGAAGGCCTCGCCGCTGTGATGATGCGCCGGGATGTTCGCCATTTGGT
GCGCTAGAAAGTTGGGTTCG

Downstream 100 bases:

>100_bases
ACAGATCGTCAGTACGCCGCAGTGTGTCGGTGTGCGTCGTAGAACAGGGTAACGTGCAATGCCAGATGTGACGCTTACCA
TTGATGGAGTGCAGGTAACT

Product: NADH-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MGLSEHIVMRELDIPDISDFAVYRRHGGWEAYRYALTEKTPADIVQMVKDSGLRGRGGAGFPTGIKWGFLPKGVYPRYLL
CNCDESEPGTFNNHQIIDKNPHQLIEGVALSAYAIEANTAYIYMRGEFAAAARTLERAIAQAYEAGFLGKNIFGTGFDLD
IYVHRGAGAYICGEETALMESLEGKIGQPRLKPPFPAVAGLYGKPTIINNVETLANVPRIVEKGVAWYRSHGTEKSPGTK
CFSLSGHVNRPGNYELPFGVPLRELIESPEYGGGMKGGRPVKIIIPGGASAPWLTADHLDVPLDYESVAAAGSMLGSGAV
IVLNDTVSAPHVAYKMDEFFKHESCGKCTPCREGTHWLVRTLHRMVEEGHATAADIDTLHSIYRQMAGNCFCLLGESAVM
PIKSALQLFPHEFEALVKRSSSRNGHHIPLAVHR

Sequences:

>Translated_434_residues
MGLSEHIVMRELDIPDISDFAVYRRHGGWEAYRYALTEKTPADIVQMVKDSGLRGRGGAGFPTGIKWGFLPKGVYPRYLL
CNCDESEPGTFNNHQIIDKNPHQLIEGVALSAYAIEANTAYIYMRGEFAAAARTLERAIAQAYEAGFLGKNIFGTGFDLD
IYVHRGAGAYICGEETALMESLEGKIGQPRLKPPFPAVAGLYGKPTIINNVETLANVPRIVEKGVAWYRSHGTEKSPGTK
CFSLSGHVNRPGNYELPFGVPLRELIESPEYGGGMKGGRPVKIIIPGGASAPWLTADHLDVPLDYESVAAAGSMLGSGAV
IVLNDTVSAPHVAYKMDEFFKHESCGKCTPCREGTHWLVRTLHRMVEEGHATAADIDTLHSIYRQMAGNCFCLLGESAVM
PIKSALQLFPHEFEALVKRSSSRNGHHIPLAVHR
>Mature_433_residues
GLSEHIVMRELDIPDISDFAVYRRHGGWEAYRYALTEKTPADIVQMVKDSGLRGRGGAGFPTGIKWGFLPKGVYPRYLLC
NCDESEPGTFNNHQIIDKNPHQLIEGVALSAYAIEANTAYIYMRGEFAAAARTLERAIAQAYEAGFLGKNIFGTGFDLDI
YVHRGAGAYICGEETALMESLEGKIGQPRLKPPFPAVAGLYGKPTIINNVETLANVPRIVEKGVAWYRSHGTEKSPGTKC
FSLSGHVNRPGNYELPFGVPLRELIESPEYGGGMKGGRPVKIIIPGGASAPWLTADHLDVPLDYESVAAAGSMLGSGAVI
VLNDTVSAPHVAYKMDEFFKHESCGKCTPCREGTHWLVRTLHRMVEEGHATAADIDTLHSIYRQMAGNCFCLLGESAVMP
IKSALQLFPHEFEALVKRSSSRNGHHIPLAVHR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=398, Percent_Identity=46.4824120603015, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI260656005, Length=398, Percent_Identity=46.4824120603015, Blast_Score=360, Evalue=2e-99,
Organism=Escherichia coli, GI1788620, Length=409, Percent_Identity=49.6332518337408, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17531727, Length=374, Percent_Identity=48.6631016042781, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19920794, Length=384, Percent_Identity=47.1354166666667, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI45550452, Length=385, Percent_Identity=46.4935064935065, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24653819, Length=402, Percent_Identity=37.5621890547264, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=36.056338028169, Blast_Score=224, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 47410; Mature: 47279

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSEHIVMRELDIPDISDFAVYRRHGGWEAYRYALTEKTPADIVQMVKDSGLRGRGGAG
CCCCCCEEEEECCCCCCHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHHHCCCCCCCCCC
FPTGIKWGFLPKGVYPRYLLCNCDESEPGTFNNHQIIDKNPHQLIEGVALSAYAIEANTA
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHEEEEEECCEE
YIYMRGEFAAAARTLERAIAQAYEAGFLGKNIFGTGFDLDIYVHRGAGAYICGEETALME
EEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHH
SLEGKIGQPRLKPPFPAVAGLYGKPTIINNVETLANVPRIVEKGVAWYRSHGTEKSPGTK
HHHCCCCCCCCCCCCHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
CFSLSGHVNRPGNYELPFGVPLRELIESPEYGGGMKGGRPVKIIIPGGASAPWLTADHLD
EEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCC
VPLDYESVAAAGSMLGSGAVIVLNDTVSAPHVAYKMDEFFKHESCGKCTPCREGTHWLVR
CCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
TLHRMVEEGHATAADIDTLHSIYRQMAGNCFCLLGESAVMPIKSALQLFPHEFEALVKRS
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCHHHHHHHHHHC
SSRNGHHIPLAVHR
CCCCCCEEEEEECC
>Mature Secondary Structure 
GLSEHIVMRELDIPDISDFAVYRRHGGWEAYRYALTEKTPADIVQMVKDSGLRGRGGAG
CCCCCEEEEECCCCCCHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHHHCCCCCCCCCC
FPTGIKWGFLPKGVYPRYLLCNCDESEPGTFNNHQIIDKNPHQLIEGVALSAYAIEANTA
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHEEEEEECCEE
YIYMRGEFAAAARTLERAIAQAYEAGFLGKNIFGTGFDLDIYVHRGAGAYICGEETALME
EEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHH
SLEGKIGQPRLKPPFPAVAGLYGKPTIINNVETLANVPRIVEKGVAWYRSHGTEKSPGTK
HHHCCCCCCCCCCCCHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
CFSLSGHVNRPGNYELPFGVPLRELIESPEYGGGMKGGRPVKIIIPGGASAPWLTADHLD
EEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEECCCCC
VPLDYESVAAAGSMLGSGAVIVLNDTVSAPHVAYKMDEFFKHESCGKCTPCREGTHWLVR
CCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
TLHRMVEEGHATAADIDTLHSIYRQMAGNCFCLLGESAVMPIKSALQLFPHEFEALVKRS
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCHHHHHHHHHHC
SSRNGHHIPLAVHR
CCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]